期刊
BIOTECHNOLOGY LETTERS
卷 37, 期 9, 页码 1765-1771出版社
SPRINGER
DOI: 10.1007/s10529-015-1856-7
关键词
Baculovirus; Porcine circovirus type 2; Sf9 cell; Translational enhancers; Virus-like particles
资金
- National Natural Science Foundation of China [31101803/C1803]
- Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences [SKLVBF201405]
To investigate the effect of three translational enhancers for enhancing transgene expression in baculovirus expression vector system using GFP as a reporter gene and selected translational enhancers to increase porcine circovirus type 2 (PCV2) VLPs production. P10UTR (the 3'-untranslated region from the baculovirus p10 gene), Syn21 (a synthetic AT-rich 21-bp sequence) and P10UTR/Syn21 increased the GFP yield by 1.4-, 4- and 4.8-fold, respectively. While IVS (intron from Drosophila myosin heavy chain gene) decreased the GFP yield by 65 %. Moreover, the synergy of P10UTR/Syn21 increased the yield of PCV2 VLPs by 4.1 fold (45 mu g/10(6) cells) compared with standard baculovirus vector. The synergy of P10UTR/Syn21 is a potential strategy to improve the recombinant vaccine production besides PCV2 VLPs in BEVS.
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