期刊
CELLULAR SIGNALLING
卷 28, 期 1, 页码 120-129出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cellsig.2015.10.011
关键词
Chemokine; cAMP; Biosensor; Adenylate cyclase; Gi; Forskolin
类别
资金
- Agence Nationale de la Recherche [09-PIRI-0003-01, 12-EMMA-0050]
- European Community's Framework Program FP7 [241440]
- INSERM
- UPMC
- CNRS
Chemokine receptors are members of the G-protein-coupled receptor (GPCR) family coupled to members of the Gi class, whose primary function is to inhibit the cellular adenylate cyclase. We used a cAMP-related and PICA-based luminescent biosensor (GloSensor (TM) F-22) to monitor the real-time downstream response of chemokine receptors, especially CX3CR1 and CXCR4, after activation with their cognate ligands CX3CL1 and CXCL12. We found that the amplitudes and kinetic profiles of the chemokine responses were conserved in various cell types and were independent of the nature and concentration of the molecules used for cAMP prestimulation, including either the adenylate cyclase activator forskolin or ligands mediating Gs-mediated responses like prostaglandin E2 or beta-adrenergic agonist. We conclude that the cAMP chemokine response is robustly conserved in various inflammatory conditions. Moreover, the cAMP-related luminescent biosensor appears as a valuable tool to analyze the details of Gi-mediated cAMP-inhibitory cellular responses, even in native conditions and could help to decipher their precise role in cell function. (C) 2015 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据