Article
Biochemistry & Molecular Biology
Irene Peinado, Isabel Moya, Laura Garcia-Valverde, Raquel Frances, Rosana Ribes, Patrocinio Polo, Maria Jose Gomez-Torres, Ana Monzo
Summary: This study explores the impact of in vitro maturation (IVM) medium and vitrification on the survival and development of germinal vesicle (GV) oocytes obtained from stimulated cycles. The results show that a higher percentage of oocytes reach the MII stage if they are vitrified before undergoing IVM. However, the significance of these differences depends on the medium used and sample size. Similar survival rates and development to blastocysts were observed in all conditions studied.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Endocrinology & Metabolism
Mausumi Das, Weon-Young Son
Summary: In vitro maturation (IVM) is a viable option for specific patients, including those at risk of ovarian hyperstimulation syndrome (OHSS), in need of urgent fertility preservation, or when controlled ovarian stimulation is not possible. The clinical applications of IVM are expanding rapidly and it is now classified as a non-experimental technique. IVM offers advantages over conventional IVF, such as reduced stimulation and risk of OHSS, shorter treatment times, and lower costs. However, challenges remain in terms of technical expertise and lower success rates compared to traditional IVF. Ongoing research aims to refine protocols and improve the efficiency and effectiveness of IVM.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Genetics & Heredity
Ashley Wiltshire, Renata Schaal, Fang Wang, Tiffany Tsou, Wilson McKerrow, David Keefe
Summary: This study investigated the impact of DMSO-containing cryoprotectant on the transcriptome and transposable elements (TE) of human oocytes. The results showed significant changes in the oocyte transcriptome, including TE alterations, after vitrification with DMSO. These findings suggest potential negative effects of the current oocyte vitrification process on gene expression and genomic stability.
Article
Reproductive Biology
Tamas Somfai, Yuji Hirao
Summary: An optimized vitrification protocol using protein-free media was established for the cryopreservation of immature bovine oocytes, which resulted in high blastocyst quality without noticeable toxic effects. Both NCSU-37 and TCM-199 were equally effective as base media for vitrification.
Article
Endocrinology & Metabolism
Qigui Xie, Yaxin Xing, Jianhong Zhou, Ling Wang, Jie Wu, Ri-Cheng Chian
Summary: Supplementation of lysophosphatidic acid in culture medium significantly improves in vitro human oocyte maturation. RNA sequencing identified potential pathways, such as TNF signaling and cell cycle, that may be associated with the enhanced oocyte maturation.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Alejandro Gonzalez-Plaza, Cristiano Brullo, Josep M. Cambra, Manuela Garcia, Eleonora Iacono, Inmaculada Parrilla, Maria Antonia Gil, Emilio A. Martinez, Cristina A. Martinez, Cristina Cuello
Summary: This study aimed to investigate the effects of different equilibration times with cryoprotectants on viability and metaphase plate morphology of vitrified-warmed porcine mature oocytes, and to evaluate the effects of melatonin supplementation during in vitro maturation on these parameters. The results showed that vitrified porcine mature oocytes equilibrated for 60 or 80 min exhibited the highest viability and similar metaphase plate characteristics to fresh controls. Furthermore, supplementation with melatonin during in vitro maturation had no effect on these parameters.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Obstetrics & Gynecology
Evelyn E. Telfer, Claus Yding Andersen
Summary: The cryopreservation of ovarian tissue is widely used to preserve fertility in high-risk females. Besides transplantation, in vitro maturation of oocytes from ovarian tissue can also be used to restore fertility safely in cases where transplantation is contraindicated.
FERTILITY AND STERILITY
(2021)
Article
Biochemistry & Molecular Biology
Alejandro de la Fuente, Charles Scoggin, Etta Bradecamp, Soledad Martin-Pelaez, Machteld van Heule, Mats Troedsson, Peter Daels, Stuart Meyers, Pouya Dini
Summary: This study aimed to elucidate the transcriptome dynamics of oocyte maturation by comparing transcriptomic changes during in vitro maturation in both oocytes and their surrounding cumulus cells. The results showed an association between oocyte maturation and pathways relating to nuclear maturation in oocytes and GTPase activity, extracellular matrix organization, and collagen trimers in cumulus cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Jianhua Li, Jing Chen, Tiecheng Sun, Shuiwen Zhang, Tingting Jiao, Ri-Cheng Chian, Youzhu Li, Ye Xu
Summary: In vitro oocyte maturation (IVM) does not affect the quality of embryos and the aneuploidy rate of embryos. The aneuploidy rate of embryos increases with maternal age, especially over 35 years old, whether in IVF or IVM group. Embryos with poor quality, especially grade III embryos, are recommended to be tested by PGS.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Buo-Jia Lu, Ya-Li Huang, Yung-Liang Liu, Brian Shiian Chen, Bou-Zenn Lin, Chi-Huang Chen
Summary: This study evaluated the outcomes of solid surface-vitrified (SSV) immature testicular tissue (ITT) grafts in vitro and in vivo, and found that the grafts remained intact and supported spermatogenesis after transplantation, suggesting the great potential of SSV for fertility preservation in pediatric cancer survivors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Reproductive Biology
G. Clerico, G. Taminelli, J. C. Veronesi, J. Polola, N. Pagura, C. Pinto, M. Sansinena
Summary: Oocyte vitrification in horses increases ROS levels, which can be mitigated by melatonin to improve mitochondrial function and ICSI embryo development. However, the impact of melatonin on pregnancy rates requires further investigation.
Article
Genetics & Heredity
S. E. Pors, D. Nikiforov, J. Cadenas, Z. Ghezelayagh, Y. Wakimoto, L. A. Z. Jara, J. Cheng, M. Dueholm, K. T. Macklon, E. M. Flachs, L. S. Mamsen, S. G. Kristensen, C. Yding Andersen
Summary: This study aimed to investigate the impact of oocyte diameter and cumulus cell mass on the potential for final maturation of immature human oocytes in vitro. The results showed that the oocyte diameter was positively associated with a higher incidence of MII, and the size of the cumulus cell mass was significantly associated with a larger oocyte diameter and the MII stage.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Obstetrics & Gynecology
Adi Kuperman Shani, Lilach Marom Haham, Hanna Balakier, Iryna Kuznyetsova, Siamak Bashar, Erin N. Day, Clifford L. Librach
Summary: The study compares the developmental competence of immature oocytes matured through in vitro rescue maturation (IVM) and their siblings matured in vivo. The results show that the fertilization rates of IVM-matured oocytes are comparable to their in vivo matured counterparts, but the cleavage and blastulation rates are lower. IVM embryos exhibit some developmental abnormalities but can still result in healthy live births.
FERTILITY AND STERILITY
(2023)
Article
Oncology
Irma Virant-Klun, Jure Bedenk, Nina Jancar
Summary: The study showed that in vitro maturation of immature oocytes after controlled ovarian stimulation is a reliable method to enhance the chances of conception in cancer patients. Cancer patients had fewer immature oocytes with lower maturity rates compared to patients with fertility problems, yet there was no significant difference in fertilization and embryo development between in vitro and in vivo matured oocytes after ICSI.
RADIOLOGY AND ONCOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Hiep Thi Nguyen, Tamas Somfai, Yuji Hirao, Thanh Quang Dang-Nguyen, Nguyen Viet Linh, Bui Xuan Nguyen, Nhung Thi Nguyen, Hong Thi Nguyen, Van Hanh Nguyen, Hiroyuki Kaneko, Mitsuhiro Takagi, Kazuhiro Kikuchi
Summary: The efficacy of dibutyryl-cAMP and roscovitine as meiotic inhibitors during in vitro maturation of vitrified porcine oocytes was compared. Roscovitine was more effective in preventing premature meiosis, but compromised subsequent embryo development, while dibutyryl-cAMP improved blastocyst development.
ANIMAL SCIENCE JOURNAL
(2022)