期刊
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
卷 58, 期 2, 页码 379-388出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/ejcts/ezaa043
关键词
Lung transplantation; Bronchoalveolar lavage; Cytokines; Primary graft dysfunction
资金
- Research Fund-Flanders [FWO 12G8715N]
- KU Leuven University [C24/15/030, C16/19/005]
- UZ Leuven [STG 015/23]
- FWO
- UZ Leuven Clinical Research Fund [C24/18/073]
OBJECTIVES: Primary graft dysfunction (PGD) remains a major post-transplant complication and is associated with increased morbidity and mortality. Mechanisms evoking PGD are not completely clear, but inflammation plays a central role. We investigated the association between PGD and inflammatory proteins present in immediate postoperative bronchoalveolar lavage. METHODS: All double-lung recipients transplanted at our institution from 2002 to 2018 were included in our study. We retrospectively selected 80 consecutive lung transplant recipients with different PGD grades (n=20 for each PGD grades 0-1 to 2-3). In bronchoalveolar lavage performed within the first 24h after donor aortic cross-clamping following lung transplantation, concentrations of 30 cytokines, chemokines and growth factors were assessed by enzyme-linked immunosorbent assay (ELISA) and correlated with donor and recipient demographics and outcomes. For analysis, 2 groups were defined: 'mild' PGD (grade 0-1) and 'severe' PGD (grades 2-3). RESULTS: Significant differences between mild and severe PGD were found in 8 biomarkers [interleukin (IL)-6, IL-10, IL-13, eotaxin, granulocyte colony-stimulating factor, interferon gamma, macrophage inflammatory protein 1 alpha, surfactant protein D (SP-D); P<0.05]. Increased IL-10 and IL-13, but none of the other proteins, were associated with short-term outcome (longer time to extubation; P=0.005 and P<0.0001; increased intensive care unit stay; P=0.012 and P<0.0001; and hospital stay; P=0.041 and P=0.002). There were no significant differences in donor and recipient characteristics between the groups. CONCLUSIONS: Expression profiles of key inflammatory mediators in bronchoalveolar lavage fluid differed significantly between lung transplant recipients with severe versus mild PGD and correlated with clinical outcome variables. Further research should focus on the early mechanisms leading to PGD.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据