期刊
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
卷 39, 期 2, 页码 780-789出版社
KARGER
DOI: 10.1159/000447788
关键词
Cardiac hypertrophy; Resveratrol; Endoplasmic reticulum stress; Apoptosis
资金
- National Natural Science Foundation of China [81100170]
- High-level personnel project of Harbin [2014SYYRCY02-5]
- Research Foundation of Qiqihar Medical University [QY2016Z-14, QY2016B-07]
Background/Aims: Resveratrol (Res), a polyphenol antioxidant found in red wine, has been shown to playa cardioprotective role. This study was undertaken to investigate whether Res can protect the heart suffering from hypertrophy injuries induced by isoproterenol (ISO), and whether the protective effect is mediated by endoplasmic reticulum (ER) stress. Methods: Cardiomyocytes were randomly assigned to the control group, ISO group (100 nM ISO for 48 h), Res + ISO group (50 mu M Res and 100 nM ISO for 48 h) and Res group (50 mu M Res for 48h only). Hypertrophy was estimated by measuring the cell surface area and the atrial natriuretic peptide (ANP) gene expression. Apoptosis was measured using Hoechst 33258 staining and transmission electron microscopy. Protein expression of ER stress and apoptosis factors was analyzed using Western Blot analysis. Results: Res effectively suppress the cardiomyocytes hypertrophy and apoptosis induced by ISO, characterized by the reduction of the myocardial cell surface area, the ANP gene expression, the LDH and MDA leakage amount and the rate of cell apoptosis, while decrease of the protein expression of GRP78. GRP94 and CHOP, and reverse the expression of Bcl-2 and Bax. Conclusion: In summary, Res treatment effectively suppressed myocardial hypertrophy and apoptosis at least partially via inhibiting ER stress. (C) 2016 The Author(s) Published by S. Karger AG, Basel
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