Article
Microbiology
Kun Huang, Yufei Zhang, Wenxiao Gong, Yong Yang, Lili Jiang, Lianzhong Zhao, Ying Yang, Yanming Wei, Chengfei Li, Xinglin He, Xiaomei Sun, Zhong Zou, Meilin Jin
Summary: The study revealed that IAV H5N6 downregulates PGRMC1 expression in the brain to promote virus proliferation by inhibiting RIG-I-mediated interferon production. This inhibition increases susceptibility to influenza virus infection in mice.
MICROBIOLOGY SPECTRUM
(2021)
Article
Immunology
Caiyue Ren, Tong Chen, Shishuo Zhang, Qingxia Gao, Jiahui Zou, Peng Li, Biaoxiong Wang, Yaxin Zhao, Aotian Ouyang, Sizhu Suolang, Hongbo Zhou
Summary: This study investigated the gene expression profiles in response to swine H1N1/2009 influenza A virus infection in newborn pig trachea cells. The researchers found that PLK3, a member of the PLK family, was upregulated after infection and played a crucial role in viral replication.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Virology
Hui Yang, Wendy Winkler, Xiaopeng Wu
Summary: IFI35 interacts with influenza virus protein NS1 to regulate RIG-I-mediated antiviral immunity, playing a crucial role in the defense against viral infection.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Vanessa Gerlt, Juliane Mayr, Juliana Del Sarto, Stephan Ludwig, Yvonne Boergeling
Summary: The study demonstrates the importance of PP2A in efficient replication of various IAV subtypes, as decreased PP2Ac levels result in reduced cell viability and increased cell death after IAV infection. This is attributed to a synergistic action of hyper-activated PI3K/Akt, MAPK/JAK-STAT, and NF-kB signaling pathways related to apoptosis, indicating the critical role of PP2A in orchestrating cell survival mechanisms during IAV infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jie Min, Ying Cao, Haizhou Liu, Di Liu, Wenjun Liu, Jing Li
Summary: This study profiles the expression of circRNA, microRNA, and mRNA in human lung carcinoma cells in response to avian influenza virus (AIV) or human influenza A virus (IAV) infection. It identifies specific circRNAs that are up-regulated by AIV infection and demonstrates their role in regulating viral replication and proliferation by affecting the Endocytosis pathway and the Cell cycle process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Julia Steinberg, Timo Wadenpohl, Stephanie Jung
Summary: The generation of endogenous RIG-I ligand (eRL) is induced by viral infection, amplifying the innate immune response. While eRL was detected in IAV infected cells, it was not found in VSV infected cells.
Article
Microbiology
Marisa Mendes, Alistair B. Russell
Summary: Defective interfering particles, parasitic variants within diverse viral populations, have an advantage during genome replication but can be excluded during population expansion; the size of deletions is not linked to innate immune response, yet replication of defective segments can enhance their immunostimulatory properties.
Article
Biochemistry & Molecular Biology
Zhi-Da Zhang, Bing Wen, Da-Jia Li, Dan-Xia Deng, Xiao-Dong Wu, Yin-Wei Cheng, Lian-Di Liao, Lin Long, Geng Dong, Li-Yan Xu, En-Min Li
Summary: In this study, we identified a novel phosphorylation site in Fascin and confirmed AKT2 as the kinase for this site. Phosphorylation of Fascin Threonine 403 suppresses the behavior of esophageal cancer cells, actin-bundling activity, and filopodia formation.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Virology
Xianfeng Hui, Lei Cao, Ting Xu, Lianzhong Zhao, Kun Huang, Zhong Zou, Peilei Ren, Haiying Mao, Ying Yang, Shuo Gao, Xiaomei Sun, Xian Lin, Meilin Jin
Summary: This study identifies the host protein PSMD12 as an important regulator of influenza virus replication, through its interaction with M1 and mediation of K63-linked ubiquitination. The findings provide novel insights into the interactions between IAV and host cells, and suggest PSMD12 as a potential target for antiviral drug development.
JOURNAL OF VIROLOGY
(2022)
Article
Immunology
Anna Kula, Edyta Makuch, Marta Lisowska, Patryk Reniewicz, Tomasz Lipinski, Jakub Siednienko
Summary: Viral infection triggers the innate immune system to produce pro-inflammatory cytokines through pattern recognition receptors. The role of Pellino3 in antibacterial and antiviral response is important but not well understood. This study investigates the involvement of Pellino3 in the RIG-I-dependent signaling pathway during influenza B virus infection. The results suggest that Pellino3 mediates the ubiquitination and degradation of TRAF3, thereby suppressing IRF3 activation and IFNβ production.
Article
Microbiology
Weigang Ren, Shuli Pei, Wenming Jiang, Meixia Zhao, Le Jiang, Honggang Liu, Yongxiang Yi, Mizhou Hui, Junwei Li
Summary: A replication-deficient recombinant influenza virus was developed in this study, which stimulated robust immune response and conferred complete protection against H5N1 or H9N2 influenza virus infections in mice.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Rosario Sabariegos, Laura Albentosa-Gonzalez, Blanca Palmero, Pilar Clemente-Casares, Eugenio Ramirez, Carlos Garcia-Crespo, Isabel Gallego, Ana Isabel de avila, Celia Perales, Esteban Domingo, Antonio Mas
Summary: This study identified specific amino acids of the NS5B protein phosphorylated by cellular kinases, which may affect HCV RNA polymerase activity and lead to viral inactivation. Results from RNA transfection experiments with mutant viruses showed that T53E/A and S282E/A mutations had an impact on viral titers.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Lin Tang, Haiyan Yan, Weibin Wu, Dawei Chen, Zhenxiong Gao, Jinqiang Hou, Cunlong Zhang, Yuyang Jiang
Summary: A series of novel substituted polycyclic pyridone derivatives were synthesized and found to exhibit potent anti-influenza activity, including inhibiting the replication of influenza A virus at picomolar concentrations. These compounds showed low cytotoxicity and promising potential as anti-influenza drug candidates.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Virology
Shufang Fan, Chunyang Gu, Huihui Kong, Lizheng Guan, Gabriele Neumann, Yoshihiro Kawaoka
Summary: Small animal models are crucial for studying the properties of seasonal and pandemic influenza viruses, but they do not always replicate human influenza viruses efficiently. Researchers have developed a high-yield vaccine virus backbone that can increase the replication of seasonal influenza viruses in Syrian hamsters, providing a useful tool for evaluating the biological properties of influenza viruses.
Article
Virology
Sunanda Baidya, Yoko Nishimoto, Seiichi Sato, Yasuhiro Shimada, Nozomi Sakurai, Hirotaka Nonaka, Koki Noguchi, Mizuki Kido, Satoshi Tadano, Kozo Ishikawa, Kai Li, Aoi Okubo, Taisho Yamada, Yasuko Orba, Michihito Sasaki, Hirofumi Sawa, Hiroko Miyamoto, Ayato Takada, Takashi Nakamura, Akinori Takaoka
Summary: THGP has been found to downregulate the production of type I interferon during viral infections, reducing sensitivity to viral infections and directly restricting viral replication. The discovery of its antiviral effects and therapeutic implications suggest a potential use in treating viral infections.
Article
Microbiology
Liang Cui, Weinan Zheng, Minghui Li, Xiaoyuan Bai, Wenxian Yang, Jing Li, Wenhui Fan, George Fu Gao, Lei Sun, Wenjun Liu
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Microbiology
Wenhui Fan, Pengtao Jiao, He Zhang, Teng Chen, Xintao Zhou, Yu Qi, Lei Sun, Yingli Shang, Hongfei Zhu, Rongliang Hu, Wenjun Liu, Jing Li
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Biology
Weinan Zheng, Liang Cui, Minghui Li, Yun Li, Wenhui Fan, Limin Yang, Jing Li, Lei Sun, Wenjun Liu
Summary: The study identified a novel strain of influenza A virus with cold sensitivity conferred by a mutation in the phosphorylation site within the nucleoprotein. The mutation led to reduced replicative capacity of the virus in low temperatures, attributed to diminished NP oligomerization. This suggests that the phosphorylation of NP may play a critical role in regulating the temperature sensitivity of influenza A virus.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Immunology
Zhenling Ma, Wenwen Zhang, Wenhui Fan, Yaru Wu, Menghao Zhang, Jun Xu, Wenqing Li, Lei Sun, Wenjun Liu, Wei Liu
Summary: This study revealed the critical role of FoxO1 as a negative regulator in RIG-I-triggered signaling, influencing type I interferon production by controlling multiple pathways, thereby affecting the antiviral immune response.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Cell Biology
Xiaoyuan Bai, Wenxian Yang, Xiaohan Luan, Huizi Li, Heqiao Li, Deyu Tian, Wenhui Fan, Jing Li, Beinan Wang, Wenjun Liu, Lei Sun
Summary: During influenza A epidemics, bacterial coinfection is a major cause of increased morbidity and mortality. Cyclophilin A (CypA) is shown to play a critical role in promoting group A Streptococcus (GAS) coinfection by regulating the FAK/Akt signaling pathway, revealing a potential therapeutic target for preventing secondary bacterial infections during influenza A outbreaks.
Article
Infectious Diseases
Wenhui Fan, Ying Cao, Pengtao Jiao, Ping Yu, He Zhang, Teng Chen, Xintao Zhou, Yu Qi, Lei Sun, Di Liu, Hongfei Zhu, Wenjun Liu, Rongliang Hu, Jing Li
Summary: Research shows that different tissues cooperate in responding to African swine fever virus infection, coordinating defense against ASFV through an inflammatory cytokine storm and interferon activation. The lung and spleen are mainly involved in the innate immune response pathway; the liver and kidney are involved in the metabolic regulatory pathway and inflammatory response; the lymph nodes cooperate with the liver to complete energy metabolism regulation.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Xiaohan Luan, Wenxian Yang, Xiaoyuan Bai, Heqiao Li, Huizi Li, Wenhui Fan, He Zhang, Wenjun Liu, Lei Sun
Summary: CypA positively regulates the IL-6 trans-signaling pathway by inhibiting degradation of IL-6 membrane receptor gp130 and enhancing its dimerization, while its expression is negatively regulated by SOCS1. SOCS1 interacts with CypA and promotes its degradation through ubiquitination, thus inhibiting the IL-6 trans-signaling pathway.
Article
Virology
Wenqiang Sun, He Zhang, Wenhui Fan, Lihong He, Teng Chen, Xintao Zhou, Yu Qi, Lei Sun, Rongliang Hu, Tingrong Luo, Wenjun Liu, Jing Li
Summary: Our study identified key proteins involved in the T cell immune response of pigs infected with ASFV and selected three high-frequency positive epitopes, providing valuable information for the development of an ASFV vaccine. The specific T cell and humoral immune responses to ASFV were found to gradually increase during infection, with higher levels observed in surviving pigs.
Article
Cell Biology
Wenxian Yang, Xiaoyuan Bai, Xiaohan Luan, Jie Min, Xiaodong Tian, Heqiao Li, Huizi Li, Wenqiang Sun, Wei Liu, Wenhui Fan, Wenjun Liu, Lei Sun
Summary: The inflammatory response is regulated by complex and delicate mechanisms, with Cyclophilin A (CypA) playing a role in promoting both inflammation activation and resolution by affecting IL-1 beta production and the epithelial-mesenchymal transition (EMT).
Article
Microbiology
Wenxian Yang, Xiaoyuan Bai, Heqiao Li, Huizi Li, Wenhui Fan, He Zhang, Wenjun Liu, Lei Sun
Summary: Epithelial-mesenchymal transition (EMT) is an important mechanism for lung tissue repair after injury. Excessive EMT caused by influenza virus infection can lead to pulmonary fibrosis and respiratory failure. This study found that different strains of influenza viruses induce varying degrees of EMT, with influenza A virus triggering stronger EMT compared to influenza B virus. This difference is attributed to the higher ability of neuraminidase in influenza A virus to activate latent TGF-beta.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Xiaoyuan Bai, Wenxian Yang, Heqiao Li, Yuna Zhao, Wenhui Fan, He Zhang, Wenjun Liu, Lei Sun
Summary: CsA regulates macrophage polarization and attenuates influenza virus-induced inflammatory responses by decreasing pro-inflammatory cytokines expression, increasing anti-inflammatory cytokines expression, and altering the polarization of macrophages from pro-inflammatory M1 phenotype to anti-inflammatory M2 phenotype. These effects depend on the binding of CsA to CypA.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Wenqiang Sun, Lihong He, Huicong Lou, Wenhui Fan, Limin Yang, Gong Cheng, Wenjun Liu, Lei Sun
Summary: Despite the availability of SARS-CoV-2 vaccines, the emergence of new variants and immune evasion remain significant challenges. This study focused on developing more effective antigens and evaluating their cross-protective immunity against different SARS-CoV-2 variants.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Pengtao Jiao, Shuchao Wang, Wenhui Fan, He Zhang, Hongyan Yin, Yingli Shang, Hongfei Zhu, Wenjun Liu, Rongliang Hu, Lei Sun
Summary: African swine fever (ASF) is a highly contagious and deadly disease in pigs, for which there is currently no vaccine or antiviral treatment available. This study evaluated the efficacy of an interferon (IFN) cocktail in preventing and treating ASF. The IFN cocktail delayed the onset of ASF symptoms and replication of ASF virus (ASFV) by one week, but did not prevent death. It increased the expression of IFN-stimulated genes (ISGs) and modulated the expression of pro- and anti-inflammatory cytokines, reducing tissue injury in ASFV-infected pigs.
ANTIVIRAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Heqiao Li, Wenxian Yang, Huizi Li, Xiaoyuan Bai, He Zhang, Wenhui Fan, Wenjun Liu, Lei Sun
Summary: In this study, three PROTACs targeting CypA were designed to enhance its ubiquitination and degradation, leading to reduced inflammation and improved survival rates in mice infected with influenza B virus.
Article
Biochemistry & Molecular Biology
Wenqiang Sun, Lihong He, He Zhang, Xiaodong Tian, Zhihua Bai, Lei Sun, Limin Yang, Xiaojuan Jia, Yuhai Bi, Tingrong Luo, Gong Cheng, Wenhui Fan, Wenjun Liu, Jing Li
Summary: The study developed an mRNA vaccine based on ferritin-formed nanoparticles for SARS-CoV-2, showing adequate protection and broad-spectrum neutralizing antibodies in mice, with the mRNA template easily modifiable to address viral mutations.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)