Heritability of targeted gene modifications induced by plant-optimized CRISPR systems

In Silico Meets In Vivo : Towards Computational CRISPR-Based sgRNA Design

(2017) Guo-hui Chuai et al.   TRENDS IN BIOTECHNOLOGY

Efficient targeted mutagenesis in soybean by TALENs and CRISPR/Cas9

(2016) Hongyang Du et al.   JOURNAL OF BIOTECHNOLOGY

Efficiency and Inheritance of Targeted Mutagenesis in Maize Using CRISPR-Cas9

(2016) Jinjie Zhu et al.   Journal of Genetics and Genomics

Efficient Targeted Genome Modification in Maize Using CRISPR/Cas9 System

(2016) Chao Feng et al.   Journal of Genetics and Genomics

Gene-edited CRISPR mushroom escapes US regulation

(2016) Emily Waltz   NATURE

Site-directed mutagenesis in Petunia × hybrida protoplast system using direct delivery of purified recombinant Cas9 ribonucleoproteins

(2016) Saminathan Subburaj et al.   PLANT CELL REPORTS

Cenh3: An Emerging Player in Haploid Induction Technology

(2016) Anne B. Britt et al.   Frontiers in Plant Science

Exploiting the CRISPR/Cas9 System for Targeted Genome Mutagenesis in Petunia

(2016) Bin Zhang et al.   Scientific Reports

Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants

(2016) Yuriko Osakabe et al.   Scientific Reports

CRISPR/Cas9-mediated mutagenesis of the RIN locus that regulates tomato fruit ripening

(2015) Yasuhiro Ito et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

The CRISPR/Cas9 system for plant genome editing and beyond

(2015) Luisa Bortesi et al.   BIOTECHNOLOGY ADVANCES

Targeted genome modifications in soybean with CRISPR/Cas9

(2015) Thomas B Jacobs et al.   BMC BIOTECHNOLOGY

Plant germline formation: common concepts and developmental flexibility in sexual and asexual reproduction

(2015) A. Schmidt et al.   DEVELOPMENT

A Simple CRISPR/Cas9 System for Multiplex Genome Editing in Rice

(2015) Chun Wang et al.   Journal of Genetics and Genomics

Efficient Virus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System

(2015) Zahir Ali et al.   Molecular Plant

High-Efficiency Genome Editing in Arabidopsis Using YAO Promoter-Driven CRISPR/Cas9 System

(2015) Liuhua Yan et al.   Molecular Plant

A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants

(2015) Xingliang Ma et al.   Molecular Plant

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

(2015) Je Wook Woo et al.   NATURE BIOTECHNOLOGY

Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications inArabidopsis

(2015) Yanfei Mao et al.   PLANT BIOTECHNOLOGY JOURNAL

Efficient targeted mutagenesis in potato by the CRISPR/Cas9 system

(2015) Shaohui Wang et al.   PLANT CELL REPORTS

A multiplex CRISPR/Cas9 platform for fast and efficient editing of multiple genes in Arabidopsis

(2015) Zhengjing Zhang et al.   PLANT CELL REPORTS

Parameters affecting frequency of CRISPR/Cas9 mediated targeted mutagenesis in rice

(2015) Masafumi Mikami et al.   PLANT CELL REPORTS

Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice

(2015) Masafumi Mikami et al.   PLANT MOLECULAR BIOLOGY

Cas9-Guide RNA Directed Genome Editing in Soybean

(2015) Zhongsen Li et al.   PLANT PHYSIOLOGY

A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

(2015) Levi G. Lowder et al.   PLANT PHYSIOLOGY

Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA

(2015) Sergei Svitashev et al.   PLANT PHYSIOLOGY

Auxin binding protein 1 (ABP1) is not required for either auxin signaling orArabidopsisdevelopment

(2015) Yangbin Gao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

(2015) Kabin Xie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Targeted mutagenesis in soybean using the CRISPR-Cas9 system

(2015) Xianjun Sun et al.   Scientific Reports

A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing

(2015) Kangquan Yin et al.   Scientific Reports

Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation

(2015) Zhi-Ping Wang et al.   GENOME BIOLOGY

A decade of pollen transcriptomics

(2015) Nicholas Rutley et al.   Plant Reproduction

A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana

(2015) Wenshan Liu et al.   Science Bulletin

CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme

(2015) Jean-Michel Michno et al.   GM Crops & Food-Biotechnology in Agriculture and the Food Chain

Germline-Transmitted Genome Editing in Arabidopsis thaliana Using TAL-Effector-Nucleases

(2015) Joachim Forner et al.   PLoS One

CRISPR/Cas9-Mediated Genome Editing in Soybean Hairy Roots

(2015) Yupeng Cai et al.   PLoS One

Generation and Inheritance of Targeted Mutations in Potato (Solanum tuberosum L.) Using the CRISPR/Cas System

(2015) Nathaniel M. Butler et al.   PLoS One

Efficient CRISPR/Cas9-mediated Targeted Mutagenesis in Populus in the First Generation

(2015) Di Fan et al.   Scientific Reports

Genome editing assessment using CRISPR Genome Analyzer (CRISPR-GA)

(2014) M. Guell et al.   BIOINFORMATICS

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

(2014) Hui-Li Xing et al.   BMC PLANT BIOLOGY

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

(2014) Hiroshi Nishimasu et al.   CELL

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

The CRISPR–Cas system for plant genome editing: advances and opportunities

(2014) Vinay Kumar et al.   JOURNAL OF EXPERIMENTAL BOTANY

Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells

(2014) Lior Nissim et al.   MOLECULAR CELL

Genome-Wide Prediction of Highly Specific Guide RNA Spacers for CRISPR–Cas9-Mediated Genome Editing in Model Plants and Major Crops

(2014) Kabin Xie et al.   Molecular Plant

CRISPR-P: A Web Tool for Synthetic Single-Guide RNA Design of CRISPR-System in Plants

(2014) Yang Lei et al.   Molecular Plant

High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells

(2014) Yuexin Zhou et al.   NATURE

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

(2014) Yanpeng Wang et al.   NATURE BIOTECHNOLOGY

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice

(2014) Huanbin Zhou et al.   NUCLEIC ACIDS RESEARCH

CRISPR/Cas9-Mediated Targeted Mutagenesis in the Liverwort Marchantia polymorpha L.

(2014) Shigeo S. Sugano et al.   PLANT AND CELL PHYSIOLOGY

The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation

(2014) Hui Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

DNA Replicons for Plant Genome Engineering

(2014) N. J. Baltes et al.   PLANT CELL

Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering inArabidopsis thaliana

(2014) Friedrich Fauser et al.   PLANT JOURNAL

Hairy Root Transformation Using Agrobacterium rhizogenes as a Tool for Exploring Cell Type-Specific Gene Expression and Function Using Tomato as a Model

(2014) M. Ron et al.   PLANT PHYSIOLOGY

Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System

(2014) C. Brooks et al.   PLANT PHYSIOLOGY

Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles

(2014) Youbong Hyun et al.   PLANTA

Efficient CRISPR/Cas9-Mediated Gene Editing in Arabidopsis thaliana and Inheritance of Modified Genes in the T2 and T3 Generations

(2014) WenZhi Jiang et al.   PLoS One

Targeted Genome Editing of Sweet Orange Using Cas9/sgRNA

(2014) Hongge Jia et al.   PLoS One

Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis

(2014) Z. Feng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Precision Genome Engineering and Agriculture: Opportunities and Regulatory Challenges

(2014) Daniel F. Voytas et al.   PLOS BIOLOGY

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Efficient genome editing in plants using a CRISPR/Cas system

(2013) Zhengyan Feng et al.   CELL RESEARCH

Targeted mutagenesis in rice using CRISPR-Cas system

(2013) Jin Miao et al.   CELL RESEARCH

Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System

(2013) Zhen Liang et al.   Journal of Genetics and Genomics

Self-processing of ribozyme-flanked RNAs into guide RNAsin vitroandin vivofor CRISPR-mediated genome editing

(2013) Yangbin Gao et al.   Journal of Integrative Plant Biology

Application of the CRISPR–Cas System for Efficient Genome Engineering in Plants

(2013) Yanfei Mao et al.   Molecular Plant

RNA-Guided Genome Editing in Plants Using a CRISPR–Cas System

(2013) Kabin Xie et al.   Molecular Plant

Biotechnology: Thirty years of transgenic plants

(2013) Wim Grunewald et al.   NATURE

Staying on target with CRISPR-Cas

(2013) Dana Carroll   NATURE BIOTECHNOLOGY

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

(2013) Jian-Feng Li et al.   NATURE BIOTECHNOLOGY

Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

(2013) Vladimir Nekrasov et al.   NATURE BIOTECHNOLOGY

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

(2013) Vikram Pattanayak et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Targeted genome modification of crop plants using a CRISPR-Cas system

(2013) Qiwei Shan et al.   NATURE BIOTECHNOLOGY

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice

(2013) Wenzhi Jiang et al.   NUCLEIC ACIDS RESEARCH

Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system

(2013) Khaoula Belhaj et al.   Plant Methods

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

RNA-Guided Genome Editing for Target Gene Mutations in Wheat

(2013) Santosh Kumar Upadhyay et al.   G3-Genes Genomes Genetics

The catalytic subunit of Arabidopsis DNA polymerase   ensures stable maintenance of histone modification

(2012) Y. Hyun et al.   DEVELOPMENT

The Development of Efficient Plant Promoters for Transgene Expression Employing Plant Virus Promoters

(2012) Sunita Patro et al.   Molecular Plant

RNA-guided genetic silencing systems in bacteria and archaea

(2012) Blake Wiedenheft et al.   NATURE

High-efficiency TALEN-based gene editing produces disease-resistant rice

(2012) Ting Li et al.   NATURE BIOTECHNOLOGY

Transcription Activator-Like Effector Nucleases Enable Efficient Plant Genome Engineering

(2012) Y. Zhang et al.   PLANT PHYSIOLOGY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Egg Cell-Secreted EC1 Triggers Sperm Cell Activation During Double Fertilization

(2012) S. Sprunck et al.   SCIENCE

CRISPR-Cas Systems in Bacteria and Archaea: Versatile Small RNAs for Adaptive Defense and Regulation

(2011) Devaki Bhaya et al.   Annual Review of Genetics

Dicer recognizes the 5′ end of RNA for efficient and accurate processing

(2011) Jong-Eun Park et al.   NATURE

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection

(2011) Vikram Pattanayak et al.   NATURE METHODS

Virus-induced gene silencing can persist for more than 2 years and also be transmitted to progeny seedlings in Nicotiana benthamiana and tomato

(2011) Muthappa Senthil-Kumar et al.   PLANT BIOTECHNOLOGY JOURNAL

New dimensions for VIGS in plant functional genomics

(2011) Muthappa Senthil-Kumar et al.   TRENDS IN PLANT SCIENCE

The Mechanism of Double-Strand DNA Break Repair by the Nonhomologous DNA End-Joining Pathway

(2010) Michael R. Lieber   Annual Review of Biochemistry

YAO is a nucleolar WD40-repeat protein critical for embryogenesis and gametogenesis in Arabidopsis

(2010) Hong-Ju Li et al.   BMC PLANT BIOLOGY

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

(2010) M. Christian et al.   GENETICS

Identification of genes expressed in the angiosperm female gametophyte

(2010) G. N. Drews et al.   JOURNAL OF EXPERIMENTAL BOTANY

Haploid plants produced by centromere-mediated genome elimination

(2010) Maruthachalam Ravi et al.   NATURE

Virus-Based MicroRNA Expression for Gene Functional Analysis in Plants

(2010) Y. Tang et al.   PLANT PHYSIOLOGY

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

High-frequency modification of plant genes using engineered zinc-finger nucleases

(2009) Jeffrey A. Townsend et al.   NATURE

Precise genome modification in the crop species Zea mays using zinc-finger nucleases

(2009) Vipula K. Shukla et al.   NATURE

Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

(2009) J. Boch et al.   SCIENCE

Technical Advance: Tobacco rattle virus as a vector for analysis of gene function by silencing

(2003) Frank Ratcliff et al.   PLANT JOURNAL