期刊
CELLULAR & MOLECULAR IMMUNOLOGY
卷 14, 期 7, 页码 621-630出版社
CHIN SOCIETY IMMUNOLOGY
DOI: 10.1038/cmi.2015.106
关键词
EMT; ERK/GSK-3 beta/Snail signaling; MCF-7; migration
类别
资金
- National Natural Science Foundation of China [11272083, 31470906, 11502049, 81471785, 31470959]
- Sichuan Youth Science and Technology Foundation of China [2014JQ0008]
Monocyte chemoattractant protein-1 (MCP-1) is a chemotactic cytokine that can bind to its receptor cysteine-cysteine chemokine receptor 2 (CCR2) and plays an important role in breast cancer cell metastasis. However, the molecular mechanisms underlying MCP-1-induced alterations in cellular functions during tumor progression are poorly understood. Here, we showed that MCP-1 stimulated the epithelial-mesenchymal transition (EMT) and induced the tumorigenesis of breast cancer cells by downregulating E-cadherin, upregulating vimentin and fibronectin, activating matrix metallopeptidase-2 (MMP-2), and promoting migration and invasion. Moreover, MCP-1 treatment reduced glycogen synthase kinase-3 beta (GSK-3 beta) activity via the MEK/ERK-mediated phosphorylation of serine-9 in MCF-7 cells. The inhibition of MEK/ERK by U0126 attenuated the MCP-1-induced phosphorylation of GSK-3 beta and decreased the expression of Snail, an EMT-related transcription factor, leading to the inhibition of MCF-7 cell migration and invasion. Inactivation of GSK-3 beta by LiCl (lithium chloride) treatment notably increased MMP-2 activity, vascular endothelial growth factor expression and EMT of MCF-7 cells. These findings revealed that MCP-1-induced EMT and migration are mediated by the ERK/GSK-3 beta/Snail pathway, and identified a potential novel target for therapeutic intervention in breast cancer.
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