Review
Biochemistry & Molecular Biology
Francieli Colussi, Hector Rodriguez, Michele Michelin, Jose A. Teixeira
Summary: The economically viable alternative of agricultural by-product bioethanol provides a sustainable solution for expanding the use of renewable energy, ensuring greater energy supply security, and reducing environmental impacts. Improving the efficiency of existing processes is crucial to reduce costs, industrial waste, and carbon footprint. Ionic liquids (ILs) have shown promise in achieving cleaner processes and efficient dissolution and fractionation of lignocellulose for biofuel production. This review analyzes recent advances in biofuel production from lignocellulosic biomass using ILs as pretreatment and highlights cost optimization techniques for robust conversion processes.
Article
Biotechnology & Applied Microbiology
Xiaoling Xian, Lv Fang, Yongxing Zhou, Biying Li, Xiaojie Zheng, Yao Liu, Xiaoqing Lin
Summary: In this study, a novel ternary deep eutectic solvent (DES) was developed to enhance component separation and enzymatic saccharification of wheat straw (WS), leading to the production of ethanol. The DES treatment effectively removed xylan and lignin from WS, resulting in high cellulose recovery and glucose yield. The maximum ethanol yield of the DES-treated WS was also improved.
FERMENTATION-BASEL
(2022)
Article
Agricultural Engineering
Jikai Zhao, Yang Yang, Meng Zhang, Charles W. Rice, Donghai Wang
Summary: The effects of switchgrass particle sizes on the effectiveness of H2SO4 and NaOH pretreatments were investigated. Increasing particle size led to higher contents of glucan, xylan, and lignin in raw switchgrass. SEM images and FTIR spectra showed no visible variations in microstructures and chemical bonds among different particle sizes under the same pretreatment conditions. The optimal ethanol conversion performance depends on the interaction between the pretreatment method and biomass particle size.
BIOMASS & BIOENERGY
(2022)
Article
Biotechnology & Applied Microbiology
Marttin Paulraj Gundupalli, Santi Chuetor, Kraipat Cheenkachorn, Kittipong Rattanaporn, Pau-Loke Show, Yu-Shen Cheng, Malinee Sriariyanun
Summary: This study investigated the interference of wax in different lignocellulosic biomasses and found that dewaxing can improve the composition and functional groups of biomass, promote dissociation of lignocellulose fibrils, and increase the extraction of reducing sugars and ethanol yield. The wax obtained from the biomasses mainly consisted of FAME, alcohol, and alkane.
BIOENGINEERING-BASEL
(2021)
Article
Agricultural Engineering
Michael Lugo-Pimentel, Emy Leblanc, Simon Kelley, Jean-Michel Lavoie
Summary: The purpose of this study is to investigate the operating conditions of a physicochemical pretreatment process for lignocellulosic biomass using homogeneous acid catalysts. Four parameters were examined: moisture content, acid catalyst, type of biomass, and reactor morphology. Different types of biomass were processed with sulfuric acid in a meat grinder, and the results showed that biomass moisture above 17% yielded less than 50% glucose. Sulfuric acid exhibited the best performance with a glucose yield of 74.5%, and the glucose yield had a direct relationship with the non-carbohydrate components of biomass.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Wan Sun, Xuezhi Li, Jian Zhao, Yuqi Qin
Summary: This study focused on the fractionation of corn stover using different pretreatment strategies to enhance enzymatic digestibility and cellulosic ethanol production. The combined dilute acid and alkaline sodium sulfite pretreatment effectively separated cellulose, hemicellulose, and lignin from corn stover. The results provided valuable insights for the biorefinery of corn stover.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Qihong Zhang, Ying Chen, Weike Su
Summary: A one-step mechano-enzymatic hydrolysis method was developed for producing cellulose nanocrystals (CNCs) using liquid-assisted grinding conditions. The study revealed that the rate constant of CNC production was 10 times higher than that of reducing sugar, indicating significant differences from solution hydrolysis. Furthermore, the activities of endo-1,4-beta-d-glucanase and exo-1,4-beta-d-glucanase were mainly maintained, while the activity of beta-glucosidase was inhibited by mechanical force.
CATALYSIS SCIENCE & TECHNOLOGY
(2023)
Article
Agricultural Engineering
Jie Qiao, Haiyang Cui, Minghui Wang, Xianshen Fu, Xinyue Wang, Xiujuan Li, He Huang
Summary: This article summarizes four promising routes for assembling new industrial plants for cellulose-based fuels and chemicals, and discusses the challenges and prospects in this field.
BIORESOURCE TECHNOLOGY
(2022)
Review
Energy & Fuels
Praveen Kumar Keshav, Chandrashekhar Banoth, Srinivas Naik Kethavath, Bhima Bhukya
Summary: This article discusses the use of cotton stalk as a feedstock for lignocellulosic ethanol production. It explores the various pretreatment, saccharification, and fermentation methods and also identifies the major barriers and future perspectives in bioethanol fermentation strategies.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Agricultural Engineering
Yu Zhang, Ruonan Wang, Jinshui Yang, Liang Liu, Yi Yang, Entao Wang, Hongli Yuan
Summary: Optimization of enzyme cocktail composition is an effective approach to enhance the hydrolytic efficiency of lignocellulose. This study quantitatively formulated the optimal enzyme ratios for efficient hydrolysis of corncobs with different pretreatments, and also determined the specific types of cellulases required for each pretreatment method. The results showed that high yields of glucose and xylose can be achieved simultaneously using the optimized enzyme formulation strategy, even at high solid loading, and high isopropanol yield was also obtained without detoxification of the hydrolysate.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Review
Agricultural Engineering
Wei Wang, Duu-Jong Lee
Summary: Biomass recalcitrance poses a significant challenge for biomass utilization, leading to the development of deep eutectic solvents (DES) as a promising solvent for biomass pretreatment. Current research focuses on the fractionation and saccharification enhancement of lignocellulosic biomass using DES.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
Alonso G. Hernandez-Mendoza, Sergio Saldana-Trinidad, Sergio Martinez-Hernandez, Bianca Y. Perez-Sarinana, Magdiel Lainez
Summary: The study demonstrated the production of bioethanol from cocoa pod husk through optimized alkaline pretreatment and enzymatic hydrolysis processes. The optimal alkaline pretreatment condition was found to be 5% NaOH for 30 minutes at 120 degrees Celsius, while optimal enzymatic hydrolysis conditions were 10 FPU g(-1) substrate and 10% solid loading.
BIOMASS & BIOENERGY
(2021)
Article
Energy & Fuels
Jianming Yu, Zhaoxian Xu, Sitong Chen, Yang Yu, Chengcheng Zhang, Xiangxue Chen, Mingjie Jin
Summary: This study investigated the economics of DLCA(ch) based biorefinery for cellulosic ethanol production and found that it has lower feedstock logistics cost and higher ethanol yield compared to dilute acid pretreatment based biorefinery. When the biorefinery scale was 2000 Mg rice straw per day, the total capital investment and minimum ethanol selling price of DLCA(ch) based biorefinery were 17.9% and 30.0% lower, respectively.
Article
Agronomy
Heeyoung Park, Sang Un Park, Byeong-Kwan Jang, Jeong Jae Lee, Yong Suk Chung
Summary: Kenaf, an annual fiber crop mainly grown in India and China, is emerging as a promising bio-based energy source due to its rapid growth, CO2 absorption capabilities, and high productivity. The study evaluated 10 different cultivars of kenaf for cellulosic ethanol production, finding that ethanol yield and productivity were significantly impacted by cultivar type, rather than biomass composition. Engineering yeast to utilize xylose under pH control can maximize ethanol production from kenaf.
GLOBAL CHANGE BIOLOGY BIOENERGY
(2021)
Article
Chemistry, Multidisciplinary
Jose A. Perez Pimienta, Gabriella Papa, Jian Sun, Vitalie Stavila, Arturo Sanchez, John M. Gladden, Blake A. Simmons
Summary: Pretreatment of Agave bagasse using a biocompatible low-cost protic IL, followed by enzymatic saccharification and ethanol fermentation, can achieve high sugar conversion and ethanol yield. Optimized pretreatment conditions can improve total ethanol yields, lignin removal, and glucan conversion.
Article
Biotechnology & Applied Microbiology
Siwaporn Wannawilai, Wen-Chien Lee, Yusuf Chisti, Sarote Sirisansaneeyakul
JOURNAL OF BIOTECHNOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Jaruwan Marudkla, Wen-Chien Lee, Siwaporn Wannawilai, Yusuf Chisti, Sarote Sirisansaneeyakul
JOURNAL OF BIOTECHNOLOGY
(2018)
Article
Engineering, Chemical
Nuttapol Lerkkasemsan, Wen-Chien Lee
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2018)
Article
Crystallography
Chun-Mei Hsu, Yi-Hsun Huang, Vanita Roshan Nimje, Wen-Chien Lee, How-Ji Chen, Yi-Hao Kuo, Chung-Ho Huang, Chien-Cheng Chen, Chien-Yen Chen
Article
Energy & Fuels
Ming-Hsun Tsai, Wen-Chien Lee, Wei-Chih Kuan, Sarote Sirisansaneeyakul, Ancharida Savarajara (Akaracharanya)
ENERGY SCIENCE & ENGINEERING
(2018)
Editorial Material
Biochemical Research Methods
Wen-Chien Lee
BIOTECHNOLOGY JOURNAL
(2019)
Article
Biotechnology & Applied Microbiology
Wei-Chih Kuan, Jian-Wen Lai, Wen-Chien Lee
Summary: The study demonstrated the stability of GSH-bound magnetic nanoparticles prepared through covalent bonding, suitable for protein immobilization. The immobilized GST-sfULP1 retained a significant portion of enzyme activity before immobilization, indicating its potential for catalyzing the production of hormones for type 2 diabetes therapy.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Polymer Science
Trakarn Yimtrakarn, Watchareeya Kaveevivitchai, Wen-Chien Lee, Nuttapol Lerkkasemsan
Summary: This study extracted lignin using the microwave-assisted solvent extraction method and characterized its properties. The results showed that the yield of extracted lignin increased with the extraction time and power of the microwave, and the extraction yield with ethanol was higher than with isopropanol.
Article
Agronomy
Ying-Hsuan Tseng, Wen-Chien Lee, Kridsana Krisomdee, Waranya Natesuntorn, Sunisa Chatsurachai, Klanarong Sriroth
Summary: This study developed a method for producing Xylooligosaccharides (XOSs) from sugarcane bagasse, and successfully constructed a recombinant Escherichia coli strain for large-scale production of endoxylanase. The enzyme activity concentration in the medium was high, and a significant yield of XOSs was obtained in a 24-hour enzyme reaction.
Article
Chemistry, Physical
Chung-Ming Lu, Raju Kumar Sharma, Pin-Yun Lin, Yi-Hsun Huang, Jung-Sheng Chen, Wen-Chien Lee, Chien-Yen Chen
Summary: In this study, expired egg white was used as a template to prepare TiO2 nano-powder and doped TiO2 powders. The photocatalytic activity of the prepared powders was investigated under different conditions. The results showed that doping with fluorine improved the photocatalytic activity of TiO2 in the visible-light region and the doping amount affected the catalyst's performance.
Article
Chemistry, Physical
Chung-Ming Lu, Raju Kumar Sharma, Chin-Wen Wang, Nalonda Chatterjee, Wen-Chien Lee, Chien-Yen Chen
Summary: This study prepared F and I-doped TiO2 nanoparticle photocatalysts using the sol-gel method and tested their degradation efficiency on methylene-blue under simulated sunlight and LED visible-light. The particle size of the doped nanoparticles varied at different concentrations, and the F-doped TiO2 nanoparticles showed a higher removal efficiency at higher concentration.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Chemistry, Physical
Chanakan Thirametoakkhara, Yi-Cheng Hong, Nuttapol Lerkkasemsan, Jian-Mao Shih, Chien-Yen Chen, Wen-Chien Lee
Summary: This study demonstrates the potential of endoxylanases from alkaliphilic Bacillus halodurans for producing xylooligosaccharides (XOS) from oil palm empty fruit bunch (EFB) under high pH conditions. The combined use of Xyn45 and Xyn23 endoxylanases resulted in a higher yield of XOS, showing a synergistic effect.
Article
Biotechnology & Applied Microbiology
Jhih-Sing Lee, Cheng-Jia Lin, Wen-Chien Lee, Hsin-Yi Teng, Meng-Hsin Chuang
Summary: The addition of glycerol to Napier grass hydrolysate increased the production of succinic acid and reduced the formation of acetic acid. Ultrafiltration membrane separation and single-stage electrodialysis were effective for the separation and purification of succinic acid. The overall recovery yield of succinic acid was 74.7%+/- 4.5% with a purity of 99.4%+/- 0.1%.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2022)
Proceedings Paper
Green & Sustainable Science & Technology
A. F. P. Harahap, M. Y. Arya Ramadhan, W. C. Lee, M. Gozan
2ND INTERNATIONAL CONFERENCE ON GREEN ENERGY AND ENVIRONMENT (ICOGEE 2020)
(2020)
Article
Materials Science, Multidisciplinary
Chun-Mei Hsu, Yi-Hsun Huang, How-Ji Chen, Wen-Chien Lee, Hao-Wei Chiu, Jyoti Prakash Maity, Chien-Cheng Chen, Yi-Hao Kuo, Chien-Yen Chen
MATERIALS TODAY COMMUNICATIONS
(2018)