Article
Spectroscopy
Zhiwen Wei, Yifeng Lan, Chao Zhang, Juan Jia, Weifen Niu, Yanli Wei, Shanlin Fu, Keming Yun
Summary: A label-free Exo I-assisted fluorescence aptamer sensing platform was established for the detection of silver ions with high sensitivity and selectivity using berberine as the fluorescent probe and Exonuclease I as the background fluorescence reducing tool. Under optimized conditions, the detection limit of Ag+ was 4.4 nM with a linear range from 0.0059 mu M to 235.48 mu M, showing good recovery rates in tap water and human serum applications.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Kaiyan Fan, Junlun Zhu, Xiaowei Wu, Xiuhua Zhang, Shengfu Wang, Wei Wen
Summary: A flexible and label-free electrochemical biosensing platform based on split aptamers and exonuclease I had been developed for parallel detection of different targets. The label-free aptasensor achieved sensitive and selective detection of thrombin, adenosine triphosphate, and mercury ion.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Nanoscience & Nanotechnology
Yarong Wang, Xueling Yan, Qiming Kou, Qi Sun, Yuexin Wang, Ping Wu, Lulan Yang, Jiaming Tang, Tao Le
Summary: The label-free fluorescent aptasensor developed in this study is a promising tool for rapid and sensitive detection and quantification of SMZ in water and soil samples, showing good sensitivity and linear detection range.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Chemistry, Analytical
Yao-Yao Fan, Jun Li, Li Fan, Jie Wen, Jing Zhang, Zhi-Qi Zhang
Summary: A label-free dual-emission aptasensor based on FRET was developed for the detection of AFB1. By utilizing two fluorescent probes and a chimeric aptamer in this system, AFB1 can be quantitatively detected with high sensitivity and a low detection limit. This convenient and reliable method could be widely used for the rapid detection of other mycotoxins in complex food samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Applied
Jingwen Li, Yongming Liu, Hao Lin, Yan Chen, Zhenbo Liu, Xuming Zhuang, Chunyuan Tian, Xiuli Fu, Lingxin Chen
Summary: A label-free colorimetric method based on Exo I-assisted signal amplification with protamine as a medium was developed for analysis of kanamycin. The method, which involved manipulating a dsDNA probe tailored from an aptamer and cDNA, demonstrated high selectivity and sensitivity for target detection. The strategy showed great potential for fabricating various biosensors based on target-induced aptamer configuration changes.
Article
Biochemistry & Molecular Biology
Yiting Fan, Khalid Amin, Wendan Jing, Bo Lyu, Sainan Wang, Hongling Fu, Hansong Yu, Huanhuan Yang, Jiaxin Li
Summary: In this study, the interaction between aptamers and AFB1 was systematically studied, and an AFB1 aptamer with higher affinity was successfully developed. Additionally, a fluorescent nano-adaptor sensing platform based on the optimal aptamer was designed for rapid detection of AFB1.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Environmental Sciences
Shanshan Liu, Yanmei Chen, Zhijun Ruan, Junqi Lin, Wen Kong
Summary: The abuse of antibiotics poses a serious threat to human health. Therefore, developing a simple and sensitive method for detecting trace residues of antibiotics in the environment and food is crucial. In this study, a novel label-free fluorescent biosensing platform based on competitive coordination reaction was designed for the detection of kanamycin. The platform showed promising results in terms of analytical performance and holds great potential in food safety analysis.
ENVIRONMENTAL RESEARCH
(2022)
Article
Chemistry, Applied
Shuang Chen, Yujie Liu, Fei Zhai, Min Jia
Summary: A novel label-free fluorescence aptasensor was developed for chloramphenicol (CAP) detection by DNA fourarm junction-assisted target recycling and SYBR Green I dye-aided fluorescence-signal amplification. The method showed high sensitivity and specificity, making it suitable for chloramphenicol detection in food products.
Article
Chemistry, Analytical
Yichen Zhao, Steven Ong, Yijing Chen, Po-Jung Jimmy Huang, Juewen Liu
Summary: In this study, new aptamers with no overlapping sequences were selected by immobilizing a DNA library using oxytetracycline as the target. A label-free and dye-free fluorescent biosensor was developed by enhancing the fluorescence intensity of tetracyclines upon binding to the aptamers. Additionally, a strand-displacement biosensor was designed for detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Zhiping Song, Juan Song, Feng Gao, Xiaoping Chen, Qinghua Wang, Yanan Zhao, Xuguang Huang, Chaoyong Yang, Qingxiang Wang
Summary: In this study, an electrochemical aptasensor for the detection of cardiac troponin I was developed by utilizing the physical and electrochemical properties of ferrocene-based covalent organic framework nanosheets. The sensor exhibited ultra-low detection limit and a wide linear range, demonstrating excellent performance in practical applications, such as serum assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Koji Toma, Yui Satomura, Kenta Iitani, Takahiro Arakawa, Kohji Mitsubayashi
Summary: This study developed a long-range surface plasmon (LRSP) aptasensor for label-free and real-time measurement of vancomycin (VCM). The sensor showed high selectivity for VCM and a dynamic range that covered the therapeutic range. It also demonstrated rapid response and recovery times, allowing repeated and real-time measurements.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Zhiping Song, Juan Song, Feng Gao, Xiaoping Chen, Qinghua Wang, Yanan Zhao, Xuguang Huang, Chaoyong Yang, Qingxiang Wang
Summary: In this study, a novel electrochemical aptasensor was developed for the rapid and accurate determination of cardiac troponin I. The sensor exhibited ultra-low detection limit and a wide linear range.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Chemistry, Analytical
Hibba Yousef, Yang Liu, Lianxi Zheng
Summary: This paper reviews the recent advances in label-free electrochemical aptasensors for thrombin detection, with a focus on the application of nanomaterials and nanostructures. The performance, advantages, and limitations of these aptasensors are summarized and compared, highlighting the impact of nanomaterials and nanostructures on sensor performance.
Article
Chemistry, Analytical
Haining Cui, Keqi An, Chengquan Wang, Yangfang Chen, Suli Jia, Jing Qian, Nan Hao, Jie Wei, Kun Wang
Summary: A disposable ratiometric electrochemical aptasensor was constructed for highly sensitive detection of aflatoxin B1. Au nanoflowers on ITO surface were used for DNA anchoring and methylene blue labeling. The detection was based on the peak current intensity ratio (I-MB/I-Fc) as the signal transducer. The aptasensor exhibited a wide detection range and a low detection limit.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Spectroscopy
Masoomeh Esmaelpourfarkhani, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: This study presents a new label-free competitive time-resolved fluorescent (TRF) aptasensor for detecting Pb2+ ions. The aptasensor shows a good linear detection range and high selectivity towards Pb2+, and it has been successfully used in milk and human serum samples. The TRF aptasensor offers advantages such as short analysis time, simple operation, and low cost.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)