期刊
CELL STEM CELL
卷 19, 期 4, 页码 516-529出版社
CELL PRESS
DOI: 10.1016/j.stem.2016.07.016
关键词
-
资金
- Kyoto University Foundation
- Nagai Foundation Tokyo
- Kidney Foundation, Japan [JKFB15-4]
- UCAM
- California Institute for Regenerative Medicine (CIRM)
- Universidad Catolica San Antonio de Murcia (UCAM)
- Catharina Foundation
- Fundacion Dr. Pedro Guillen
- G. Harold and Leila Y. Mathers Charitable Foundation
- Leona M. and Harry B. Helmsley Charitable Trust [2012-PG-MED002]
- Moxie Foundation
Transit-amplifying nephron progenitor cells (NPCs) generate all of the nephrons of the mammalian kidney during development. Their limited numbers, poor in vitro expansion, and difficult accessibility in humans have slowed basic and translational research into renal development and diseases. Here, we show that with appropriate 3D culture conditions, it is possible to support long-term expansion of primary mouse and human fetal NPCs as well as NPCs derived from human induced pluripotent stem cells (iPSCs). Expanded NPCs maintain genomic stability, molecular homogeneity, and nephrogenic potential in vitro, ex vivo, and in vivo. Cultured NPCs are amenable to gene targeting and can form nephron organoids that engraft in vivo, functionally couple to the host's circulatory system, and produce urine-like metabolites via filtration. Together, these findings provide a technological platform for studying human nephrogenesis, modeling and diagnosing renal diseases, and drug discovery.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据