期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 105, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2019.103597
关键词
Ruminant; Innate immune memory; Marine yeast; Polysaccharides; Pathogen
资金
- CONACYT, Mexico [INFR-2014-01/225924, PDCPN2014-01/248033]
Beta-glucans from yeast can induce trained immunity in in vitro and in vivo models. Intraperitoneal doses of beta-glucans in mammals have shown to induce trained immunity, but the training effects of orally administering beta-glucans are unknown. Newborn goats are susceptible to infections in the neonatal stage, so the induction of trained immunity could improve animal survival. This study aimed to describe the in vitro effects of immunological training by beta-glucan from Debaryomyces hansenii (beta-Dh) on caprine monocytes, as well as its in vivo effects using oral doses on newborn goats upon challenge with lipopolysaccharide (LPS). Hence in vitro, goat monocytes trained with beta-Dh up-regulated the gene expression of macrophage surface markers (CD11b and F4/80) whereas enhanced cell survival and high phagocytic ability was found upon LPS challenge. In the in vivo experiment, newborn goats stimulated with two doses (day - 7 and - 4) of beta-Dh (50 mg/kg) and challenged (day 0) with LPS showed an increase in respiratory burst activity, IL-1 beta, IL-6, and INF alpha production in plasma, and transcription of the macrophage surface markers. This study has demonstrated for the first time that trained immunity was induced with oral doses of beta-glucan upon LPS challenge in mammals using newborn goats.
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