Article
Cell Biology
Sanghoon Lee, Rebekah Karns, Soona Shin
Summary: Hepatic progenitor cells (HPCs) play crucial roles in liver diseases and cancer, but their exact role in disease progression is not well understood. Our study found that HPCs communicate with endothelial cells through paracrine factors, promoting angiogenesis and disease progression. Modulation of HPCs differentiation status and inhibition of CSF1 signaling can suppress the paracrine function of HPCs, inhibiting angiogenesis.
CELLULAR SIGNALLING
(2022)
Article
Biochemistry & Molecular Biology
Yuecheng Guo, Jumo Zhu, Xianjun Xu, Bo Shen, Zhenyang Shen, Binghang Li, Fei Li, Tianyi Gu, Xiaobo Cai, Hui Dong, Lungen Lu
Summary: Liver injury in CDE and DDC models was associated with high YB-1 expression, proliferative HPCs, and activated autophagy. YB-1 promoted proliferation and autophagy of HPCs, with TGF-beta inducing YB-1 nuclear translocation and facilitating HPC expansion. YB-1 nuclear translocation promoted Atg7 transcription essential for TGF-beta/YB-1 mediated HPC expansion and liver fibrosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Multidisciplinary Sciences
Martin H. Berryer, Matthew Tegtmeyer, Loic Binan, Vera Valakh, Anna Nathanson, Darina Trendafilova, Ethan Crouse, Jenny A. Klein, Daniel Meyer, Olli Pietilainen, Francesca Rapino, Samouil L. Farhi, Lee L. Rubin, Steven A. Mccarroll, Ralda Nehme, Lindy E. Barrett
Summary: Emerging evidence and the relative inaccessibility of human brain tissue highlight the usefulness of human pluripotent stem cell technologies for studying human astrocytes. Researchers have developed a rapid and scalable method for generating functional human induced astrocytes (hiAs) that exhibit typical astrocyte characteristics and can be used for disease modeling. These hiAs provide a valuable resource for studying astrocyte function and dysfunction.
Review
Immunology
Tian Lan, Shuaijie Qian, Chengwei Tang, Jinhang Gao
Summary: The biliary system comprised of cholangiocytes plays a crucial role in maintaining liver function. Robust biliary repair is initiated in disease conditions, with immune cells playing an important role in this process.
FRONTIERS IN IMMUNOLOGY
(2022)
Letter
Biochemistry & Molecular Biology
Sana Raza, Sangam Rajak, Jyotika Srivastava, Archana Tewari, Pratima Gupta, Bandana Chakravarti, Sujoy Ghosh, Chandra P. Chaturvedi, Rohit A. Sinha
Summary: In this study, autophagy genes were found to play a novel role in regulating telomere extension and maintenance pathways. The results demonstrate that autophagy directly influences cellular longevity through regulation of telomerase activity.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Cell & Tissue Engineering
Yuting Lin, Fang Zhang, Ludi Zhang, Lian Chen, Shan Zheng
Summary: This study investigates the characteristics of SOX9-positive liver progenitor-like cells (LPLCs) in Biliary Atresia (BA) and finds that LPLCs are associated with disease progression and prognostic factors. LPLCs have superior proliferation and anti-senescence ability under cholestasis conditions and continuously differentiate into reactive ductular cells (RDCs) from hepatocytes.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Takeshi Katsuda, Jonathan Sussman, Jinyang Li, Allyson J. Merrell, William P. Vostrejs, Anthony Secreto, Juntaro Matsuzaki, Takahiro Ochiya, Ben Z. Stanger
Summary: In recent years, a method called chemically induced liver progenitors (CLiPs) has been developed to give adult hepatocytes the ability to proliferate in vitro. However, there is a controversy surrounding the origin of these cells. This study provides lineage tracing-based evidence that adult hepatocytes and biliary epithelial cells can acquire proliferative capacity using the CLiP method, and both types of cells can differentiate into hepatocyte-like cells.
Article
Cell Biology
Lianjie Miao, Yangyang Lu, Anika Nusrat, Hala Y. Abdelnasser, Sayantap Datta, Bin Zhou, Robert J. Schwartz, Mingfu Wu
Summary: This study found that NFPs regulate progenitor cell differentiation, cardiomyocyte proliferation, and trabecular morphogenesis partially through Notch1 during cardiac development. Deletion of Notch1 or RBPJk in the MDKO did not fully rescue structural defects, but partially rescued defects in cardiac progenitor cell differentiation, cardiomyocyte proliferation, and trabecular morphogenesis.
Article
Cell & Tissue Engineering
Jiwan Choi, Seoon Kang, Bitnara Kim, Seongjun So, Jongsuk Han, Gyeong-Nam Kim, Mi-Young Lee, Seonae Roh, Ji-Yoon Lee, Soo Jin Oh, Young Hoon Sung, Yeonmi Lee, Sung Hoon Kim, Eunju Kang
Summary: The study established an efficient hepatic differentiation protocol for AM-MSCs, replacing fetal bovine serum with polyvinyl alcohol to improve differentiation efficiency, and demonstrated the potential of AM-HPCs as transplantable therapeutic materials for liver disease treatment.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Pharmacology & Pharmacy
Yasukazu Takanezawa, Yui Kashiwano, Ryosuke Nakamura, Yuka Ohshiro, Shimpei Uraguchi, Masako Kiyono
Summary: Chronic exposure to methylmercury is associated with obesity and metabolic syndromes. This study investigated the effects of continuous exposure to 0.5μM methylmercury on adipocyte differentiation in 3T3-L1 cells. The results showed that methylmercury enhanced triglyceride content and the expression of adipocyte differentiation markers, particularly during the later stages of differentiation.
Article
Cell Biology
Ziqi Guo, Shiming Pu, Yun Li, Xiaoxia Wang, Suying Hu, Hongxia Zhao, Cheng Yang, Zuping Zhou
Summary: Researchers successfully isolated two subsets of HSPCs from adult mouse liver and found that these cells exhibited robust proliferative activity and differentiation potential. These findings may provide a new theoretical basis for stem cell therapy in liver diseases.
JOURNAL OF MOLECULAR HISTOLOGY
(2022)
Article
Multidisciplinary Sciences
Yue Xiao, Min Hu, Qiyan Lin, Ting Zhang, Siying Li, Linjuan Shu, Xiuli Song, Xiaoyong Xu, Wentong Meng, Xue Li, Hong Xu, Xianming Mo
Summary: This study reveals that Dopey2 and Pcdh7 regulate the proliferation and differentiation of neural stem cells and progenitors during embryonic neurogenesis, leading to the proper size and architecture of zebrafish brains. Dopey2 promotes the proliferation of neural stem cells/progenitors, while Pcdh7 promotes their differentiation. These proteins work together to orchestrate the development and arrangement of neural cells in zebrafish embryonic brains.
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Article
Gastroenterology & Hepatology
Ju Hee Oh, Waqar Khalid Saeed, Hye Young Kim, Seung Min Lee, A. Hyeon Lee, Gye Ryeol Park, Eileen L. Yoon, Dae Won Jun
Summary: This study found that MLKL level is higher in patients with non-alcoholic fatty liver disease. Hepatic stellate cells (HSCs) avoid necroptosis and are activated through autophagosome and NF-kappa B pathways under necroptotic stimuli.
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Veterinary Sciences
Woo Keyoung Kim, Yeon Sung Son, Ji-Hey Lim, Wan Hee Kim, Byung-Jae Kang
Summary: This study demonstrates the successful isolation and propagation of NSPCs derived from the canine cervical spinal cord, which have the potential to differentiate into neuronal and glial cells.
BMC VETERINARY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Wen-ting Liu, Ying-ying Jing, Lu Gao, Rong Li, Xue Yang, Xiao-rong Pan, Yang Yang, Yan Meng, Xiao-juan Hou, Qiu-dong Zhao, Zhi-peng Han, Li-xin Wei
CELL DEATH AND DIFFERENTIATION
(2020)
Article
Biotechnology & Applied Microbiology
Fei Ye, Jinghua Jiang, Chen Zong, Xue Yang, Lu Gao, Yan Meng, Rong Li, Qiudong Zhao, Zhipeng Han, Lixin Wei
Article
Cell Biology
Shanmin Zhao, Jinghua Jiang, Yingying Jing, Wenting Liu, Xue Yang, Xiaojuan Hou, Lu Gao, Lixin Wei
CELL DEATH & DISEASE
(2020)
Article
Biochemistry & Molecular Biology
Jianxin Qian, Lin Xu, Wenlong Yu, Xiaoqiang Gu, Yongjun Zuo, Ying Chen, Feng Xie, Lixin Wei
CELL AND BIOSCIENCE
(2020)
Article
Oncology
Yan Meng, Qiudong Zhao, Liwei An, Shi Jiao, Rong Li, Yan Sang, Jianping Liao, Pingping Nie, Fuping Wen, Junyi Ju, Zhaocai Zhou, Lixin Wei
Summary: This study elucidates the importance of the TNFR2-hnRNPK-YAP signaling axis in hepatic progenitor cells during the development of primary liver cancer, as well as their upregulated expressions in PLC tissues and their association with poor prognosis.
Article
Biotechnology & Applied Microbiology
Jianxing Zeng, Yingying Jing, Qionglan Wu, Jinhua Zeng, Lixin Wei, Jingfeng Liu
Summary: The study demonstrates that autophagy regulates hepatic differentiation of hepatic progenitor cells through the Wnt signaling pathway.
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Cell Biology
Xiaojuan Hou, Changchun Shao, Kai Sun, Rong Li, Lu Gao, Yan Meng, Yingying Jing, Lixin Wei
Summary: Autophagy deficiency in liver cancer cells leads to increased DNA damage, partly due to downregulation of the DNA repair enzyme MGMT. Overexpression of MGMT can reverse the chemosensitivity of liver cancer cells under autophagy deficiency.
Article
Cell & Tissue Engineering
Changchun Shao, Xue Yang, Yingying Jing, Xiaojuan Hou, Yihua Huang, Chen Zong, Lu Gao, Wenting Liu, Jinghua Jiang, Fei Ye, Junxia Shi, Qiudong Zhao, Rong Li, Xiaoren Zhang, Lixin Wei
Summary: The study reveals a correlation between LPS/TLR4/YAP1 signaling and cell stemness, indicating that LPS plays a role in maintaining the stemness of hepatocytes in the PV area. LPS can facilitate the dedifferentiation of mature hepatocytes into progenitor-like cells for potential liver injury repair.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Oncology
Xue Yang, Changchun Shao, Lixia Duan, Xiaojuan Hou, Yihua Huang, Lu Gao, Chen Zong, Wenting Liu, Jinghua Jiang, Fei Ye, Junxia Shi, Qiudong Zhao, Dong Wu, Lixin Wei
Summary: The study found that Oncostatin M (OSM) plays an important role in hepatocarcinogenesis by regulating liver inflammation, potentially serving as a target for the prevention and treatment of HCC, as well as an indicator for HCC prognosis.
Article
Biotechnology & Applied Microbiology
Xiaojuan Hou, Wenting Liu, Xue Yang, Changchun Shao, Lu Gao, Li Zhang, Lixin Wei
Summary: This study found that extracellular microparticles derived from apoptotic hepatic progenitor cells can efficiently kill hepatoma-initiating cells and prevent hepatocarcinogenesis.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Cell Biology
Yan Meng, Yan Sang, Jianping Liao, Qiudong Zhao, Shuping Qu, Rong Li, Jinghua Jiang, Meifeng Wang, Jiahong Wang, Dong Wu, Chun Cheng, Lixin Wei
Summary: Liver cancer patients exhibit transcriptional diversity in tumor cells, which is associated with stemness and potentially driven by tumor-associated fibroblasts (TAF). Specifically, TAF interacts with heterogeneous tumor cells via COL1A1-ITGA2, and COL1A1-mediated YAP activation is mechanistically linked to increased transcriptional diversity. The levels of COL1A1, ITGA2, and YAP are also correlated with morphological heterogeneity and poor overall survival of liver cancer patients.
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Changchun Shao, Yingying Jing, Shanmin Zhao, Xue Yang, Yiming Hu, Yan Meng, Yihua Huang, Fei Ye, Lu Gao, Wenting Liu, Dandan Sheng, Rong Li, Xiaoren Zhang, Lixin Wei
Summary: Sox9(+)HNF4 alpha(+) hepatocytes are generated by transition from mature hepatocytes and play an important role in liver regeneration, while Bcl3 activation promotes hepatocyte conversion and liver regeneration.
CELL DEATH & DISEASE
(2022)
Article
Oncology
Shuping Qu, Xiaobing Zhang, Yutian Wu, Yan Meng, Hongyu Pan, Qiang Fang, Lei Hu, Jin Zhang, Ruoyu Wang, Lixin Wei, Dong Wu
Summary: This study compared the efficacy and safety of a combination therapy of transcatheter arterial chemoembolization (TACE), Lenvatinib, and programmed cell death protein-1 (PD-1) inhibitors with TACE alone in patients with unresectable hepatocellular carcinoma (uHCC). The combination therapy showed higher tumor response rate, longer progression-free survival, and overall survival compared to TACE alone. The neutrophil-to-lymphocyte ratio was identified as an independent factor affecting survival.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yanping Wei, Xuewu Tang, Yibin Ren, Yun Yang, Fengliang Song, Jingbo Fu, Shuowu Liu, Miao Yu, Jing Chen, Suyang Wang, Kecheng Zhang, Yexiong Tan, Zhipeng Han, Lixin Wei, Baohua Zhang, Zhangjun Cheng, Liang Li, Hongyang Wang
Summary: The study identified potential HMGB1-driven RNA-RNA crosstalk networks in HCC and explored their roles at different stages of the disease, suggesting the potential for RNA-based precision therapy. Interventions against HMGB1 and RICTOR in HCC cells demonstrated the potential to enhance the effectiveness of immunotherapy.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Xue Yang, Yan Meng, Zhipeng Han, Fei Ye, Lixin Wei, Chen Zong
CELL AND BIOSCIENCE
(2020)