Article
Biochemistry & Molecular Biology
Cristina Martinez-Valiente, Cristian Garcia-Ruiz, Beatriz Roson, Alessandro Liquori, Elisa Gonzalez-Romero, Raul Fernandez-Gonzalez, Isabel Gomez-Redondo, Jose Cervera, Alfonso Gutierrez-Adan, Alejandra Sanjuan-Pla
Summary: Mutations in splicing factors, such as U2AF1, in conjunction with mutations in epigenetic factors have been identified in myelodysplastic syndromes (MDS), indicating a cooperative role in MDS pathogenesis. Despite exhibiting reduced size, U2af1(mut/+) mice showed aberrant RNA splicing in hematopoietic stem/progenitor cells. Additionally, a double mutant U2af1(mut/+) Tet2(-/-) mice model displayed increased monogranulocytic precursors and functional alterations, but did not develop MDS within a 6-month observation period.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Vera Garcia-Outeiral, Cristina de la Parte, Miguel Fidalgo, Diana Guallar
Summary: TET2 plays a crucial role in cell fate outcomes by catalyzing demethylation on DNA and potentially through RNA hydroxymethylation. It influences transcriptional regulation, normal development, and pluripotency contexts, showcasing its versatility in genome regulation and cellular phenotypes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Jingbo Zhang, Lei Kuang, Yanyu Li, Qing Wang, Hui Xu, Jianwei Liu, Xueyan Zhou, Yang Li, Bei Zhang
Summary: This study investigates the expression of TET2 in endometrial cancer and its clinical significance, as well as the relationship between TET2 and clinicopathological features and prognosis. The findings suggest that TET2 may play an important role in EC development and is a potential prognostic marker. Additionally, this study reveals that metformin can increase the levels of TET2 and 5hmC in EC cells, potentially inhibiting cell proliferation.
FRONTIERS IN ONCOLOGY
(2022)
Article
Genetics & Heredity
Meiling Xia, Rui Yan, Wenjuan Wang, Anqi Kong, Meng Zhang, Zhigang Miao, Wei Ge, Bo Wan, Xingshun Xu
Summary: Environmental stress promotes epigenetic alterations that impact gene expression and participate in disease pathology. This study found that chronic stress increases the expression of cytoplasmic Tet2, leading to increased RNA 5hmC modification. Tet2 interacts with Upf1 and regulates the stability of stress-related genes, potentially affecting neurodevelopment.
FRONTIERS IN GENETICS
(2023)
Article
Dermatology
Changying Niu, Shenxing Tan
Summary: This study discovered new epigenetic mechanisms for keloid formation. TET2 regulates the proliferation of fibroblasts by regulating the level of 5hmC modification in the promoter region of TGF beta.
CLINICAL COSMETIC AND INVESTIGATIONAL DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yuzuru Sasamoto, Siyuan Wu, Catherine A. A. Lee, Jason Y. Jiang, Bruce R. Ksander, Markus H. Frank, Natasha Y. Frank
Summary: Epigenetic DNA modification by 5-hydroxymethylcytosine (5hmC) regulates biological functions in the human cornea. The enzyme TET2 is involved in generating 5hmC, which plays a role in the epigenetic regulation of corneal gene expression. Knockdown of TET2 in corneal epithelial cells leads to reduced 5hmC levels and repression of molecular pathways involved in corneal differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Fei-Man Hsu, Qiu Ya Wu, Emily B. Fabyanic, Alex Wei, Hao Wu, Amander T. Clark
Summary: This study investigates Ten Eleven Translocation 1 (TET1) as a regulator of DNA demethylation and its role in facilitating human primordial germ cell-like cells (hPGCLCs) competency. The findings indicate that 5-hydroxymethylcytosine (5hmC) enriches at specific binding sites in hPGCLCs and is accompanied by localized DNA demethylation. Furthermore, deleting the catalytic domain of TET1 reduces hPGCLC competency, which can be rescued by transitioning hESCs to defined media and a recombinant substrate.
Letter
Oncology
Long Chen, Anqi Ren, Yuan Zhao, Hangyu Chen, Qifang Wu, Mengzhu Zheng, Zijian Zhang, Tongcun Zhang, Wu Zhong, Jian Lin, Haichuan Zhu
Summary: In this study, the researchers discovered that TET1, a key participant in DNA epigenetic control, is highly upregulated in T-cell acute lymphoblastic leukemia (T-ALL) and is negatively correlated with patient prognosis. Knockdown of TET1 suppressed T-ALL growth and progression, suggesting that TET1 inhibition might be an effective way to treat T-ALL. Additionally, the researchers found that auranofin, a gold-containing compound, is a potent TET1 inhibitor, which can reprogram gene expression and inhibit T-ALL growth.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xuguo Zhu, Lijun Xiong, Ruitu Lyu, Yinghui Shen, Lu Liu, Shuangqi Li, Christian Argueta, Li Tan
Summary: Estrogen signaling has been found to regulate the expression of TET2 gene and the level of 5hmC, playing a role in the epigenetic regulation of breast cancer cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Xiaohua Ma, Meng Zhang, Rui Yan, Hainan Wu, Bo Yang, Zhigang Miao
Summary: beta 2SP, encoded by Sptbn1, regulates various cell functions and plays a role in myelin sheath formation, potentially affecting neuropathy caused by demyelination. Interaction between beta 2SP and TET2 modulates 5hmC levels, with potential therapeutic implications for ischemic stroke.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Chengshun Li, Chuanni Peng, Ziping Jiang, Haobo Hu, Chao Lin, Yongjian Gao, Da Liu, Baozhen Sun, Dongxu Wang
Summary: The study demonstrated that Ginkgo biloba extract (GBE) inhibited cell proliferation and invasion in human colorectal cancer (CRC) through upregulating TET2 protein expression, which is regulated by miR-29a.
DNA AND CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qi Qi, Qianqian Wang, Kailing Liu, Jiangyue Bian, Zhixuan Yu, Jian Hou
Summary: Tet dioxygenases can induce DNA demethylation and play important roles in mammalian development. Overexpression of Tet1 and Tet2 can alter the methylation landscape of zygotes and impair embryonic development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Zehu Yuan, Ling Ge, Weibo Zhang, Xiaoyang Lv, Shanhe Wang, Xiukai Cao, Wei Sun
Summary: This study identified novel isoforms associated with meat tenderness in sheep using Iso-seq and RNA-seq data. It also revealed the regulatory role of CTCF in alternative splicing and identified potential regulatory mechanisms involving methylation of the ANKRD23 gene.
Review
Biochemical Research Methods
Jiashu Liu, Cui-Xiang Lin, Xiaoqi Zhang, Zongxuan Li, Wenkui Huang, Jin Liu, Yuanfang Guan, Hong-Dong Li
Summary: Alternative splicing (AS), a key pathway for transcriptional regulation, has been shown to be associated with complex diseases. Computational approaches for detecting disease-associated AS events have been developed. This review discusses the metrics used for characterizing AS events quantitatively. It also reviews and discusses three types of methods for detecting disease-associated splicing events: differential splicing analysis, aberrant splicing detection, and splicing-related network analysis. Additionally, methods for detecting genetic variants that potentially regulate splicing are described. Experimental comparisons are conducted to illustrate the performance of each method. The limitations of these methods are discussed, as well as potential ways to address them. This review aims to provide a systematic understanding of computational approaches for analyzing disease-associated splicing.
BRIEFINGS IN BIOINFORMATICS
(2023)
Article
Biochemistry & Molecular Biology
Hui Qian, Jiaqi Zhao, Xinyi Yang, Sujuan Wu, Yang An, Yuxiu Qu, Zhen Li, Hui Ge, En Li, Wei Qi
Summary: TET1-mediated active DNA demethylation plays a crucial role in adipogenesis by regulating transcriptional activation and gene expression.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Editorial Material
Cardiac & Cardiovascular Systems
Ryan J. Marina, Xiang-Dong Fu
CIRCULATION-CARDIOVASCULAR GENETICS
(2015)
Article
Biochemistry & Molecular Biology
Ryan J. Marina, David Sturgill, Marc A. Bailly, Morgan Thenoz, Garima Varma, Maria F. Prigge, Kyster K. Nanan, Sanjeev Shukla, Nazmul Haque, Shalini Oberdoerffer
Editorial Material
Cardiac & Cardiovascular Systems
Ryan J. Marina, Xiang-Dong Fu
CIRCULATION-CARDIOVASCULAR GENETICS
(2015)
Article
Biochemical Research Methods
Yarui Diao, Rongxin Fang, Bin Li, Zhipeng Meng, Juntao Yu, Yunjiang Qiu, Kimberly C. Lin, Hui Huang, Tristin Liu, Ryan J. Marina, Inkyung Jung, Yin Shen, Kun-Liang Guan, Bing Ren
Article
Biochemical Research Methods
Kristopher W. Brannan, Isaac A. Chaim, Ryan J. Marina, Brian A. Yee, Eric R. Kofman, Daniel A. Lorenz, Pratibha Jagannatha, Kevin D. Dong, Assael A. Madrigal, Jason G. Underwood, Gene W. Yeo
Summary: The STAMP technique efficiently detects RBP-RNA interactions and reveals the dynamic regulation of RBPs in single cells. Pairing with long-read sequencing allows for isoform-specific RBP target site identification, while Ribo-STAMP can measure transcriptome-wide ribosome association in cells.
Article
Neurosciences
Kathryn H. Morelli, Qian Wu, Maya L. Gosztyla, Hongshuai Liu, Minmin Yao, Chuangchuang Zhang, Jiaxu Chen, Ryan J. Marina, Kari Lee, Krysten L. Jones, Megan Y. Huang, Allison Li, Charlene Smith-Geater, Leslie M. Thompson, Wenzhen Duan, Gene W. Yeo
Summary: Huntington's disease is a neurodegenerative disorder caused by genetic mutation. Researchers have developed a CRISPR-Cas13d system to target and eliminate the mutant RNA responsible for the disease. In animal models, this approach successfully reduced mutant protein levels and improved motor coordination without adverse effects. This study provides proof of concept for RNA-targeting CRISPR-Cas13d system as a potential therapeutic approach for Huntington's disease.
NATURE NEUROSCIENCE
(2023)
Article
Cell Biology
Ryan J. Marina, Kristopher W. Brannan, Kevin D. Dong, Brian A. Yee, Gene W. Yeo
Article
Biochemistry & Molecular Biology
Ranjan Batra, David A. Nelles, Elaine Pirie, Steven M. Blue, Ryan J. Marina, Harrison Wang, Isaac A. Chaim, James D. Thomas, Nigel Zhang, Vu Nguyen, Stefan Aigner, Sebastian Markmiller, Guangbin Xia, Kevin D. Corbett, Maurice S. Swanson, Gene W. Yeo
