Article
Chemistry, Multidisciplinary
Keiya Uezono, Risa Maeda, Makoto Yoritate, Hiroaki Matoba, Go Hirai
Summary: Direct modification of 2,3-dehydro-2-deoxy-N-acetylneuraminic acid (DANA) derivatives at the C-3 position with malonates was achieved using Mn(OAc)3-mediated oxidative coupling reaction. This method resulted in good yield and high diastereoselectivity of C3-modified DANA derivatives. We also synthesized 3-CH2COOH-DANA as a potential sialidase inhibitor, but its inhibitory activity against several sialidases was weak, possibly due to the proximity of the acidic functional group to the DANA skeleton.
Article
Biochemistry & Molecular Biology
Md Amran Howlader, Ekaterina P. Demina, Suzanne Samarani, Tianlin Guo, Antoine Caillon, Ali Ahmad, Alexey Pshezhetsky, Christopher W. Cairo
Summary: The isoenzymes of the neuraminidase family (NEU1, 3, and 4) play essential roles in the activation, extravasation, and migration of immune cells in the induction of acute inflammatory response. NEU1 and NEU3 deficiency decreases leukocyte recruitment, while NEU4 deficiency increases it, indicating opposite effects of NEU isoenzymes on leukocyte infiltration. Further investigation is needed to determine the mechanisms and importance of NEU isoenzymes as regulators of the inflammatory cascade.
Article
Multidisciplinary Sciences
Alexandra T. Linder, Michael Schmidt, Julia Hitschfel, Markus Abeln, Pascal Schneider, Rita Gerardy-Schahn, Anja K. Muenster-Kuehnel, Lars Nitschke
Summary: This study investigates the role of sialoglycans on B cells in cell migration, complement system control, and cellular signaling regulation. The results demonstrate that sialoglycans are crucial for the survival of B cells by counteracting multiple death-inducing pathways.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Genetics & Heredity
Daichi Sahashi, Yurina Kubo, Mika Ishii, Asami Ikeda, Chiharu Yamasaki, Masaharu Komatsu, Kazuhiro Shiozaki
Summary: Neu1 sialidase plays a crucial role in regulating lysosomal degradation in macrophages, thus affecting the susceptibility of fish to bacterial infection.
Article
Multidisciplinary Sciences
Stephanie Schumacher, Dirk Dedden, Roberto Vazquez Nunez, Kyoko Matoba, Junichi Takagi, Christian Biertuempfel, Naoko Mizuno
Summary: The interaction mechanism between Integrin alpha(5)beta(1) and fibronectin was studied, revealing significant conformational changes upon complex formation. Additionally, the resting state of the integrin adopts an incompletely bent conformation, challenging the existing model.
Article
Dermatology
Burcu Beksac, Laura Gleason, Sarah Baik, John M. Ringe, Pierluigi Porcu, Neda Nikbakht
Summary: This study investigated the effect of dermal fibroblasts and fibronectin on the survival and proliferation of malignant cutaneous lymphoma cells. The results showed that dermal fibroblasts increased the proliferation rates of the lymphoma cells but did not rescue them from apoptosis. Additionally, an overexpression of a fibronectin isoform was found in the lymphoma skin. The malignant cells expressed fibronectin-binding integrins and adhered to fibronectin, but adhesion-mediated survival was not observed. These findings have important implications for understanding and targeting the malignant skin stromal microenvironment in cutaneous lymphomas.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2022)
Article
Fisheries
Nhung Thi Tran, Linh Khanh Vo, Masaharu Komatsu, Kazuhiro Shiozaki
Summary: This study found that bacterial sialoglycoconjugates may be involved in the infectivity of Edwardsiella piscicida, a gram-negative bacterium that causes Edwardsiellosis in fish. Through cloning and transferring the css gene, the researchers discovered that CSS promotes infection and enhances mortality in infected zebrafish larvae. CSS also enhances bacterial attachment and motility and leads to an increase in sialo-conjugates.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Review
Immunology
Kelli Monteiro da Costa, Leonardo Marques da Fonseca, Jhenifer Santos dos Reis, Marcos Andre Rodrigues da Costa Santos, Jose Osvaldo Previato, Lucia Mendonca-Previato, Leonardo Freire-de-Lima
Summary: Chagas' disease, caused by Trypanosoma cruzi, was described by Dr. Carlos Chagas in the early 20th century. One important discovery was trans-sialidase, an enzyme that masks the parasite's presence and dampens the immune response. Research into the disease has identified key events in the biochemical mechanism of T. cruzi-host cell interactions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Immunology
Erik P. Lillehoj, Irina G. Luzina, Sergei P. Atamas
Summary: Mammalian neuraminidases (NEUs) have metabolic functions and also regulate the activity of glycoproteins. They modify protein folding, interactions, and proteolytic sites by removing sialic acid. Desialylation of mucins (MUCs) by NEUs contributes to disease regulation. NEU1 and MUC1 play important roles in disease pathogenesis.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Cell Biology
Moses Musiime, Joan Chang, Uwe Hansen, Karl E. Kadler, Cedric Zeltz, Donald Gullberg
Summary: Increased awareness of the importance of extracellular matrix composition has led to a renewed interest in understanding how cells recognize and remodel the matrix. Fibronectin and fibrillar collagens are key proteins in the matrix, with integrins directly anchoring cells to collagen and supporting collagen fibrillogenesis.
Article
Biochemistry & Molecular Biology
Md Amran Howlader, Tianlin Guo, Christopher W. Cairo
Summary: Cell migration is a crucial step in immune response, and NEU1, NEU3, and NEU4 act as positive regulators of this process. Pharmacological inhibition of these enzymes attenuates cell migration.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Biochemistry & Molecular Biology
William H. D. Bowles, Tracey M. Gloster
Summary: Sialidases and sialyltransferases play essential roles in various organisms, including humans, in recognition and cell function. Inhibitors targeting these enzymes have been used in treating diseases such as cancer, but there is an ongoing need for novel potent and specific inhibitors to combat resistance.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Carina Magdaleno, Trenton House, Jogendra S. Pawar, Sophia Carvalho, Narendiran Rajasekaran, Archana Varadaraj
Summary: This study explores the impact of fibronectin (FN) matrix on epithelial functions in the context of cancer progression, showing that FN fibril formation decreases during hypoxia, leading to reduced migratory potential. Additionally, disruption of FN fibrils in normal breast cells results in acinar luminal filling, indicating a loss of apicobasal polarity.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Immunology
Kazuya Takeda, Tomonori Kaifu, Ryunosuke Michihata, Naotaka Kinugawa, Atushi Fujioka, Ayaka Tateno, Kaoru Toshima, Hirotaka Kanoh, Kei-Ichiro Inamori, Keiju Kamijo, Toshiki Himeda, Yoshiro Ohara, Jin-Ichi Inokuchi, Akira Nakamura
Summary: Theiler's murine encephalomyelitis virus (TMEV) infects DC cells by binding to them, while pDCs are not susceptible to TMEV due to low levels of sialic acid modification on their surface. TMEV utilizes sialylated integrins as cell surface receptors to evade pDC activation.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Diego Munoz-Provencio, Maria J. Yebra
Summary: Sialic acids play a crucial role in regulating cellular and molecular processes, as well as promoting the growth of bacteria in the colon. Sialidases, enzymes that release sialic acids, have potential biotechnological applications in producing mimics of human milk oligosaccharides. Understanding the biological role of sialidases in bacteria is important for these applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Santo Anto, Venkatachalem Sathish, Chengwen Sun, Stephen T. O'Rourke
Summary: This study found that exposure to cigarette smoke extract (CSE) impairs the vasorelaxant effect of apelin in coronary arteries and reduces its ability to inhibit endothelium-dependent relaxation to acetylcholine (ACh).
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
(2022)
Article
Oncology
Zengqi Tan, Yazhuo Jiang, Liang Liang, Jinpeng Wu, Lin Cao, Xiaoman Zhou, Zhihui Song, Zhenyu Ye, Ziyan Zhao, Hui Feng, Zewen Dong, Shuai Lin, Zhangjian Zhou, Yili Wang, Xiang Li, Feng Guan
Summary: This study reveals the pro-metastatic and proliferative function of upregulated glycosyltransferase C1GLAT1, and provides preliminary data on mechanisms underlying dysregulation of C1GALT1 via miR-1-3p / cHP1BP3 axis in BLCA.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Green & Sustainable Science & Technology
Cheng-Wen Sun, Bojan Obrenovic, Hai-Ting Li
Summary: The study found that virtual CSR co-creation can promote customers' willingness to purchase green products. Virtual CSR co-creation and self-construal have an interactive effect on green product purchase intention, and environmental involvement plays a moderating role in this interaction. Virtual community identity partially mediates this interactive effect.
Review
Biochemistry & Molecular Biology
Richard Nii Lante Lamptey, Chengwen Sun, Buddhadev Layek, Jagdish Singh
Summary: Hypertension is a global health concern, and neurogenic hypertension (NH) accounts for almost half of all hypertension cases. The increased sympathetic nervous system activity mediated by angiotensin II in the brain is known to be the main cause of NH. However, traditional antihypertensive drugs lack the ability to effectively penetrate the blood-brain barrier, making it challenging to manage NH. Therefore, developing strategies for targeted delivery of antihypertensives to the brain is crucial.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Urology & Nephrology
Chengwen Sun, Dongrong Yang, Jin Zhu, Yibin Zhou, Congming Xiang, Sheng Wu
Summary: This study suggests that the modified staging system may be more effective in predicting prognosis for patients with advanced prostate cancer compared to the AJCC 8th staging system, indicating a need for further optimization.
Article
Food Science & Technology
Shijie Fu, Yanting Dang, Huilin Xu, Aimin Li, Xiaoman Zhou, Xiaodong Gao, Zijie Li
Summary: Aloe vera-fermented beverages (AFB) have been found to prevent diet-induced obesity and associated complications. They reduce lipid droplets in cells, inhibit weight gain and fat accumulation, and lower the levels of total cholesterol, alanine aminotransferase, and interleukin 10. Additionally, AFB alters the composition of gut microbiota.
Article
Pharmacology & Pharmacy
Richard Nii Lante Lamptey, Chengwen Sun, Jagdish Singh
Summary: Functionalized liposomes can safely deliver losartan potassium to the brain, blocking angiotensin receptor type 1 activity and preventing the occurrence of hypertension in spontaneously hypertensive rats.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Cell Biology
Yi-Shi Liu, Yicheng Wang, Xiaoman Zhou, Linpei Zhang, Ganglong Yang, Xiao-Dong Gao, Yoshiko Murakami, Morihisa Fujita, Taroh Kinoshita
Summary: Liu et al. demonstrate that precursors of specific GPI-anchored proteins, in collaboration with ER-resident ARV1, contribute to the upregulation of GPI biosynthesis. This study provides insights into the mechanism by which GPI biosynthesis is regulated.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Weicheng Kong, Detao Liu, Chengwen Sun, Dejun Kong
Summary: A CuCMnNiTi coating was prepared on Ti6Al4V alloy using laser cladding technique. The coating's corrosion resistance was improved by tempering at 450 and 500℃.
MATERIALS CHARACTERIZATION
(2023)
Article
Chemistry, Medicinal
Qi Zhang, Yue Shen, Sayeman Islam Niloy, Stephen T. O'Rourke, Chengwen Sun
Summary: This study found that chronic infusion of apelin-13 does not change blood pressure or heart rate, and does not alter Ang II-induced hypertension and cardiac hypertrophy. These findings suggest that an APJ receptor biased agonist could be a better therapeutic alternative for treatment of hypertension.
Article
Biology
Hui-Lin Xu, Xiaoman Zhou, Shuai Chen, Si Xu, Zijie Li, Hideki Nakanishi, Xiao-Dong Gao
Summary: The rare sugar L-sorbose has been found to decrease cell viability and increase apoptotic cells in culture, and it enhances the effect of tumor chemotherapy in mouse xenograft models. It acts as a C-3 epimer of d-fructose, and it induces apoptosis by inactivating glycolytic enzyme and impairing mitochondrial function, while also downregulating the transcription of KHK-A, which attenuates the antioxidant defense mechanism in cancer cells.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biology
Kaijun Chi, Huilin Xu, Hanjie Li, Ganglong Yang, Xiaoman Zhou, Xiao-Dong Gao
Summary: This study compared the adaptability and yield of expressing Siglec9-Fc fusion protein in HEK293 and CHO cell lines, and optimized the culture conditions. The glycosylation, yield, dimerization, and sialic acid binding activity of the Siglec9-Fc protein produced in HEK293 and CHO were analyzed. The study found that the protein yield was slightly higher in CHO compared to HEK293, and the recombinant protein from HEK293 had more fucosylation, while CHO showed more sialylation. Both products exhibited high dimerization ratio and sialic acid binding activity. Furthermore, the study analyzed the potential ligands on cancer cell lines and bladder cancer tissue. The results are important for selecting suitable Siglec9-Fc protein expression systems and detecting related ligands.
Review
Biology
Xiaoman Zhou, Kaijun Chi, Chairui Zhang, Quan Liu, Ganglong Yang
Summary: Tumor cells accumulate sialylation in tissues, which blocks receptor and ligand binding and inhibits immune activation through binding to the Siglec receptor. Blocking the sialylation-Siglec axis in tumor tissues could alleviate the immune suppression. This review provides updated insights into the function of sialylation in tumor biology and the latest developments in sialylation-targeted tumor therapeutics.
Article
Biochemistry & Molecular Biology
Fanrong Yao, Sayeman Islam Niloy, Yue Shen, Qi Zhang, Stephen T. O'Rourke, Chengwen Sun
Summary: Intravenous administration of apelin-13 causes a significant decrease in blood pressure, while not affecting heart rate and contractility. Furthermore, the plasma from apelin-treated rats induces relaxation of blood vessels through a NO-independent mechanism involving an unidentified substance.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Sayeman Islam Niloy, Yue Shen, Lirong Guo, Stephen T. O'Rourke, Chengwen Sun
Summary: This study reveals that dysfunction of BKCa channels in vascular smooth muscle cells (VSMCs) is related to abnormal proliferation and morphological changes in hypertension, possibly due to decoupling of IP3R-BKCa. Genetic disruption of IP3R-BKCa coupling increases VSMC proliferation, while infusion of the BKCa opener NS1619 attenuates vascular hypertrophy in hypertensive rats.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)