Article
Immunology
Seema Patel, Heidi R. Tucker, Himanshu Gogoi, Samira Mansouri, Lei Jin
Summary: The cGAS-STING pathway collaborates with the MyD88 pathway in monocytes to promote late-stage lung IFN gamma production during pulmonary pneumococcal infection.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Juliet N. Crabtree, Daniel R. Caffrey, Leandro de Souza Silva, Evelyn A. Kurt-Jones, Katherine Dobbs, Arlene Dent, Katherine A. Fitzgerald, Douglas T. Golenbock
Summary: Plasmodium falciparum induces trained innate immune responses in vitro, where adaptive immunity plays a partial role. Monocytes and dendritic cells are primarily responsible for the trained response, which relies on soluble signals from lymphocytes.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Immunology
Fatemeh Asgari, Domenico Supino, Raffaella Parente, Nadia Polentarutti, Matteo Stravalaci, Remi Porte, Fabio Pasqualini, Marialuisa Barbagallo, Chiara Perucchini, Camilla Recordati, Elena Magrini, Andrea Mariancini, Federica Riva, Alessia Giordano, Sadaf Davoudian, Thierry Roger, Cornelis van't Veer, Sebastien Jaillon, Alberto Mantovani, Andrea Doni, Cecilia Garlanda
Summary: The study highlights the important role of PTX3 in controlling Klebsiella pneumoniae infection by modulating inflammatory responses and tissue damage.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Mariska T. Meijer, Alex F. de Vos, Brendon P. Scicluna, Joris J. Roelofs, Cherine Abou Faycal, Gertraud Orend, Fabrice Uhel, Tom van der Poll
Summary: While TNC plays a role in shaping the immune response during lipopolysaccharide-induced inflammation, its role may be superseded by other factors during pneumosepsis caused by a common gram-negative pathogen.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Michael Petermann, Zacharias Orfanos, Julie Sellau, Mohammad Gharaibeh, Hannelore Lotter, Bernhard Fleischer, Christian Keller
Summary: Our study investigated the role of CCR2 in a self-healing mouse model of O. tsutsugamushi infection, showing that CCR2 deficiency delayed disease onset, resolved symptoms, and resulted in higher concentrations of bacteria in the lung and impaired clearance. CCR2 drives blood monocytosis and the influx and activation of monocytes into the lung, accelerating bacterial replication and development of pulmonary inflammation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Stefania Dentice Maidana, Yoshiya Imamura, Mariano Elean, Leonardo Albarracin, Keita Nishiyama, Yoshihito Suda, Shoichiro Kurata, Maria Angela Jure, Haruki Kitazawa, Julio Villena
Summary: Orally administered Lacticaseibacillus rhamnosus CRL1505 enhances respiratory immunity, providing protection against respiratory viruses and Streptococcus pneumoniae. However, its effectiveness against Gram-negative bacterial infections has not been evaluated. This study aimed to assess whether Lcb. rhamnosus CRL1505 could beneficially regulate the respiratory innate immune response and improve resistance to hypermucoviscous KPC-2-producing Klebsiella pneumoniae of the sequence type 25 (ST25).
Article
Microbiology
Kexin Liu, Shuang Tan, Weiyuan Ye, Limin Hou, Binghu Fang
Summary: This study found that iron promotes Klebsiella pneumoniae biofilm formation by increasing protein and polysaccharide concentrations in the extracellular polymeric substances. Metabolomic analysis revealed that the effect of iron is mediated through down-regulation of metabolites such as succinic acid. Addition of succinic acid counteracted the biofilm-forming effect of iron without bactericidal side effects.
Article
Virology
Bryce Dylan Schubert, Heng Ku, Mwila Kabwe, Trang Hong Nguyen, Helen Irving, Joseph Tucci
Summary: Bacterial sepsis, characterized by immunosuppression and cytokine storm, is difficult to treat clinically. The role of bacteriophages in immune modulation of sepsis is unclear. In this study, we isolated two lytic bacteriophages against K. pneumoniae that cause sepsis in humans, one of which was a novel jumbo bacteriophage. We demonstrated for the first time that these bacteriophages did not stimulate excessive production of tumor necrosis factor alpha or interleukin-6 in THP-1 monocyte cell lines with varying levels of IRAK3 expression.
Article
Biochemistry & Molecular Biology
Ying Li, Runying He, Xizhen Ge
Summary: This study demonstrated that activation of dha regulon in Klebsiella pneumoniae efficiently promotes biomass accumulation. Transcriptome analysis revealed that the regulator was activated by the supply of glycerol, leading to increased biomass without affecting antibiotic susceptibility.
PROCESS BIOCHEMISTRY
(2022)
Article
Veterinary Sciences
Zrinka Oreskovic, Lenka Leva, Katarina Chlebova, Karolina Hlavova, Radek Tesarik, Jan Gebauer, Martin Faldyna
Summary: This study investigated the effects of different cytokine microenvironments on porcine dendritic cells and found that an IFNγ-rich microenvironment induced a higher inflammatory response and release of polarizing cytokines. Different cytokine microenvironments have the potential to modulate the activation ability of dendritic cells.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Cell Biology
Yuanbang Lin, Mingwei Sheng, Hua Qin, Peng Zhang, Chunli Wang, Wei Fu, Xiangjun Meng, Duowei Wang, Yachao Hou
Summary: This study reveals the important regulatory role of Caspase 6 in liver sterile inflammatory injury. Overexpression of Caspase 6 is associated with more severe liver injury and inflammation in human liver tissues. Caspase 6 is mainly elevated in macrophages rather than hepatocytes. In mouse models, Caspase 6 deficiency protects the liver from IR injury.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Cell Biology
Sae-Hae Kim, Byeol-Hee Cho, Kwang Soon Kim, Yong-Suk Jang
Summary: C5a in the Peyer's patch enhances CD8(+) T cell responses by increasing C5/C5a levels. Single-cell RNA sequencing identifies dendritic cells expressing C5aR and lysozyme as key players in CD8(+) T cell immune induction.
Review
Biochemistry & Molecular Biology
Karolina Stojowska-Swedrzynska, Adrianna Lupkowska, Dorota Kuczynska-Wisnik, Ewa Laskowska
Summary: Klebsiella pneumoniae is a common pathogen responsible for various infections, and its multidrug resistance has been recognized as a significant public health threat. Heteroresistance, a frequent phenotype in pathogens, may be associated with an increased risk of recurrent infections and treatment failure in K. pneumoniae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Infectious Diseases
Junjun Chen, Huan Zhang, Xuelian Liao
Summary: Hypervirulent Klebsiella pneumoniae (hvKP), including multidrug-resistant hvKP (MDR-hvKP), is a global concern due to its ability to cause outbreaks with high pathogenicity and mortality. Key virulence agents associated with hvKP and MDR-hvKP include iron acquisition systems, increased capsule production, colibactin toxin, and hypermu-coviscosity. Recent advances have identified new hypervirulence factors and explored the evolution of MDR-hvKP. Further research is needed to identify reliable methods for detection and investigate innovative treatment targets.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Immunology
Jinhui Tang, Liwen Gu, Jieyu Luo, Haihua Luo, Qingli Zeng, Yong Jiang
Summary: This study confirms the protective role of 1,25(OH)2D3 in macrophages against Klebsiella pneumoniae (Kp) infection and demonstrates its promotion of autophagy activity. Inhibiting autophagy impairs the ability of 1,25(OH)2D3 to eliminate Kp infection and its anti-inflammatory effects. These protective effects are validated in a mouse model and provide evidence for further exploration of 1,25(OH)2D3 as an adjunctive therapy for bacterial infections.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)