Article
Food Science & Technology
Mengjia Xu, Zhi Li, Xiaogan Zhao, Wei Li
Summary: The study reveals the potential prebiotic properties of exopolysaccharides from Lactobacillus helveticus LZ-R-5 and L. pentosus LZ-R-17, showing their resistance to digestion, ability to be consumed by human gut microbiota, and promotion of beneficial bacterial growth for improved gut health.
Article
Chemistry, Applied
Xiaomeng Wang, Mengjia Xu, Danling Xu, Kai Ma, Changliang Zhang, Guangxian Wang, Mingsheng Dong, Wei Li
Summary: This study investigated the structure and function of exopolysaccharide (EPS) extracted from Lactobacillus helveticus SNA12. The EPS was found to be rich in galactose and glucose and contained specific repeat units. Scanning electron microscope analysis showed that the EPS had a tight structure with a smooth and uneven surface. Furthermore, the EPS exhibited prebiotic effects by modulating the gut microbiota composition and enhancing short-chain fatty acid production.
CARBOHYDRATE POLYMERS
(2022)
Article
Multidisciplinary Sciences
Miriam Zago, Lucia Massimiliano, Barbara Bonvini, Giuseppe Penna, Giorgio Giraffa, Maria Rescigno
Summary: This study investigated the functional characteristics of four strains of L. helveticus isolated from Italian hard cheeses, showing their immunomodulatory and antioxidant activities, and positive effects on the immune system.
Article
Biochemistry & Molecular Biology
Juanjuan Tian, Xiaomeng Wang, Xueliang Zhang, Cangping Zhang, Xiaohong Chen, Mingsheng Dong, Xin Rui, Qiuqin Zhang, Yong Fang, Wei Li
Summary: Two exopolysaccharide fractions (EPS1 and EPS2) were isolated from Paecilomyces cicadae TJJ 1213, with mannose and galactose as main components. Both EPS1 and EPS2 showed potential in protecting PC12 cells from oxidative stress and could potentially serve as therapeutic agents for neuronal disorders.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
Chin-Lin Huang, Hsu-Feng Chu, Chien-Chen Wu, Fu-Sheng Deng, Pei-Jun Wen, Shao-Ping Chien, Chi-Hsein Chao, Ying-Tsong Chen, Mei-Kuang Lu, Ying-Chieh Tsai
Summary: This study identified a L. fermentum strain, GR1009, which is phenotypically distinct but genetically similar to the psychobiotic strain PS150. Unlike PS150, GR1009 did not have significant hypnotic effects in mice. However, heat-killed PS150 showed similar effects to live bacteria, suggesting the involvement of a heat-stable effector, such as exopolysaccharide (EPS).
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zhiwen Ge, Xuan Bao, Zhiyu Li, Xiaohong Chen, Wei Li, Xin Rui, Junjun Wu, Qiuqin Zhang, Mingsheng Dong
Summary: This study investigated the mechanism of high viscosity in Sayram ketteki yogurt. It analyzed the growth, acidification properties, and in situ exopolysaccharides (EPS) production of Lactobacillus helveticus MB2-1 in milk medium. The microstructure of the yogurt was analyzed, and the characteristics of in situ EPS were studied using various analytical techniques. The results showed that the in situ EPS produced by L. helveticus MB2-1 were closely connected with proteins, effectively filling the three-dimensional network structure of casein clusters, resulting in high viscosity of the yogurt. The in situ EPS was determined to be a new heteropolysaccharide containing fucose, making it unique.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Iulia-Roxana Angelescu, Silvia-Simona Grosu-Tudor, Lucia-Roxana Cojoc, Gabriel-Mihai Maria, Gabriela N. Chiritoiu, Cristian V. A. Munteanu, Medana Zamfir
Summary: Traditional fermented foods in Romania can provide a rich source of novel lactic acid bacteria with functional properties, such as Lactobacillus helveticus 34.9, which has the ability to inhibit the growth of various bacteria and produce a large bacteriocin. The production of the bacteriocin is enhanced under stress conditions and its mode of action and degree of inhibition depend on the concentration and indicator strain used. The bacteriocin treatment induces morphological changes in sensitive strains, making it a potential candidate for applications in health, food, and non-food related fields.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Microbiology
Yin Liu, Shujuan Zheng, Jiale Cui, Tingting Guo, Jingtao Zhang, Bailiang Li
Summary: The study demonstrated that EPS-1 significantly alleviated the damage caused by DSS-induced UC by reducing intestinal inflammation, improving mucosal barrier function, and modulating gut microbiota composition.
Article
Food Science & Technology
Xuefang Zhao, Qi Liang
Summary: This study evaluated the effects of probiotic Lactobacillus plantarum MC5 on the quality, antioxidant activity, and storage stability of yogurt. The results showed that adding MC5 can improve the rheological properties, texture, and antioxidant activity of yogurt. The study also found that MC5 can promote the growth of Streptococcus thermophilus. Therefore, L. plantarum MC5 has potential for industrial application in milk fermentation.
Article
Biochemistry & Molecular Biology
Xiaowan Feng, Hui Zhang, Phoency F. H. Lai, Zhiqiang Xiong, Lianzhong Ai
Summary: A pyruvated exopolysaccharide named LPE-1 was isolated and characterized from the fermentation broth of Lactobacillus plantarum AR307. LPE-1 was found to contain Galp and Glcp at a molar ratio of 2:1, with a molecular weight of 605 kDa. The presence of pyruvate group in LPE-1 plays a significant role in improving the viscosity and plasticity of dairy products.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Food Science & Technology
Kaiying Jia, Min Wei, Yao He, Yujie Wang, Hua Wei, Xueying Tao
Summary: Exopolysaccharide (EPS) from probiotic Enterococcus hirae WEHI01 was isolated and purified. The EPS consists of four fractions, I01-1, I01-2, I01-3, and I01-4, with I01-2 and I01-4 being the main components. The study found that I01-2 and I01-4 could enhance macrophage-mediated immune responses, indicating their potential application in functional food and medication production.
Article
Microbiology
Muhammad Saleem, Samiullah Malik, Hafiza Mahreen Mehwish, Muhammad Waqas Ali, Nazim Hussain, Mohsin Khurshid, Muhammad Shahid Riaz Rajoka, Yougen Chen
Summary: The study demonstrated that EPS produced by Lactobacillus Plantarum S123 has promising biological features, including antibacterial, antioxidant, water holding, oil holding, flocculation, and emulsifying activities. These findings suggest its potential use in functional food products.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xin Hu, Shanshan Zhao, Fengshu Li, Xiuli Zhang, Yaping Pan, Jinren Lu, Yiming Li, Mutai Bao
Summary: This study isolated fifteen marine strains from surface sediments in the Northwest Pacific Ocean and screened for EPS production. Planococcus rifietoensis AP-5 produced the highest yield of EPS, which was purified and characterized. The purified EPS (PPS) exhibited immunomodulatory activity and had significant surface area and pore volume. This study provides valuable insights for screening marine polysaccharide-based immunomodulators.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Xi-Mei Yang, Si-Qiang Wang, Li-Sha Chen, Zhen-Yuan Zhu
Summary: A new exopolysaccharide named PC-EPS was isolated from Cordyceps cicadae. PC-EPS consists of mannose, glucose, and galactose in a ratio of 28.84:1:19.42, with an average molecular weight of 3.72 x 10(6) Da. It has the potential to enhance phagocytosis, cell proliferation, and immunological activity.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Food Science & Technology
Xiaomeng Wang, Juanjuan Tian, Xueliang Zhang, Nanyu Tang, Xin Rui, Qiuqin Zhang, Mingsheng Dong, Wei Li
Summary: This study isolated two exopolysaccharide fractions from Lacticaseibacillus paracasei GL1, which had high molecular weights and thermal stability. These fractions were found to exert immunomodulatory effects.
Review
Chemistry, Applied
Xingran Kou, Dongdong Su, Fei Pan, Xiwei Xu, Qingran Meng, Qinfei Ke
Summary: This review provides a systematic discussion of the application of molecular dynamics (MD) simulations in aroma compounds (ACs)/cyclodextrins (CDs) inclusion complexes (ICs). It covers the establishment of the simulation process, parameter selection, model evaluation, and various application cases, summarizing the major achievements and challenges of this method.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Haoyu Yu, Quanbin Zhang, Ammad Ahmad Farooqi, Jing Wang, Yang Yue, Lihua Geng, Ning Wu
Summary: Brown algae are rich in fucoidan, which has been found to have anti-cancer and anti-metastasis effects. Fucoidan inhibits tumor cell growth, proliferation, and metastasis, and also promotes immune responses in the tumor microenvironment.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Liang Dong, Yanan Li, Hailin Cong, Bing Yu, Youqing Shen
Summary: Gene therapy is a revolutionary treatment that requires suitable vectors for protecting and releasing exogenous nucleic acids in target cells. Chitosan, as a non-viral vector, has gained attention due to its good biocompatibility and ability to load large amounts of nucleic acids. This paper summarizes the potential of chitosan and its derivatives as gene delivery vector materials, discusses factors influencing transfection efficiency, performance evaluation, ways to optimize infectious efficiency, and current research development directions. It also provides an outlook on the future prospects of chitosan.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ana Iglesias-Mejuto, Beatriz Magarinos, Tania Ferreira-Goncalves, Ricardo Starbird-Perez, Carmen Alvarez-Lorenzo, Catarina Pinto Reis, Ines Ardao, Carlos A. Garcia-Gonzalez
Summary: This study developed a novel processing strategy to manufacture drug-loaded and personalized aerogels with nanostructures. The aerogels demonstrated bioactivity and antimicrobial effects, promoting bone regeneration and preventing infections in bone tissue engineering.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Zhenxu Shi, Dimei Yang, Yan Zhou, Xinyu Chen, Lin Gan, Jin Huang
Summary: This study proposes a micro-assembly method to improve the photoluminescent properties of crystalline cellulose nanocrystals (CNCs) by organizing them within a sub-micrometer-sized metal-organic framework and coating with TiO2. The TiO2 coating prevents CNC assembly breakdown and allows information to be revealed using screenprinted labels for anti-counterfeiting purposes.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Xuejiao Liu, Bin Fu, Jierong Chen, Zhenyu Sun, Dongdong Zheng, Zhonghua Li, Bing Gu, Ying Zhang, Haojie Lu
Summary: Liver disease is a major cause of global mortality, and identifying biomarkers for diagnosing its progression is crucial for improving outcomes. Targeted mass spectrometry is a powerful tool for verifying biomarker candidates and clinical applications, particularly for glycoproteins translation. However, the limitation of analyzing only one sample per run has become apparent. In this study, a high-throughput intact N-glycopeptides quantification strategy was developed, allowing the validation of 20 samples per run with an average analysis time of 3 minutes per sample. The strategy was applied in a cohort of 461 serum samples and identified a panel of 10 IgG N-glycopeptides that have strong clinical utility in evaluating the severity of liver disease.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Qian Wang, Hao Jiang, Hongli Zhang, Weiqiao Lu, Xiao Wang, Wenfeng Xu, Jia Li, Youjing Lv, Guoyun Li, Chao Cai, Guangli Yu
Summary: This study proposes a novel strategy of antibody-beta-glucan conjugates (AGC) to enhance the antitumor immune response to immune checkpoint blockade (ICB) therapy. AGC demonstrated powerful tumor suppression and promoted interaction between tumor cells and dendritic cells (DCs), thereby enhancing immunotherapeutic benefits.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Chaeyeon Lee, Sojeong Lee, Sang Wook Kang
Summary: The study aims to enhance the properties of porous membranes by addressing the limitations associated with phase separation. By using cellulose acetate and citric acid, the researchers were able to fabricate membranes with improved mechanical strength and thermal stability. The cross-linking effect of citric acid resulted in a more uniform pore structure and higher porosity.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Tejaswini Kolipaka, Giriraj Pandey, Noella Abraham, Dadi A. Srinivasarao, Rajeev Singh Raghuvanshi, P. S. Rajinikanth, Vidya Tickoo, Saurabh Srivastava
Summary: This review focuses on the design and application of polysaccharide-based hydrogel wound dressings, highlighting aspects such as biocompatibility, biodegradability, drug entrapment, moisturizing ability, swelling, and mechanical properties. Additionally, various crosslinking methods and recent developments in stimuli-responsive hydrogels are discussed.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Anqi Xu, Nan Zhang, Shixing Su, Hongyu Shi, Daoqiang Lu, Xifeng Li, Xin Zhang, Xin Feng, Zhuohua Wen, Gengwu Ma, Mengshi Huang, Chi Huang, Yuqi Hu, Hao Yuan, Qinwen Liu, Daogang Guan, Jun Wang, Chuanzhi Duan
Summary: The study presents a one-pot radical polymerization method to fabricate a hydrogel with adhesive properties, which can effectively treat bacterial-infected diabetic wounds and accelerate wound healing.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Montarop Yamabhai, Munthipha Khamphio, Thae Thae Min, Chai Noy Soem, Nguyen Cao Cuong, Waheni Rizki Aprilia, Krisanai Luesukprasert, Karsidete Teeranitayatarn, Atthaphon Maneedaeng, Tina R. Tuveng, Silje B. Lorentzen, Simen Antonsen, Paiboon Jitprasertwong, Vincent G. H. Eijsink
Summary: The study investigates the bioconversion of chitosan into soluble anti-inflammatory chitosan oligosaccharides (CHOS) using an enzyme. The results show that the generated CHOS have anti-inflammatory activity, but the magnitude of the activity depends on the substrate and production process. Different methods of dissolving chitosan also affect the properties of CHOS. The study highlights the importance of quality assurance in CHOS preparations.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Wen Cao, Jiangping Wu, Xinya Zhao, Zixu Li, Jie Yu, Taili Shao, Xuefeng Hou, Lutan Zhou, Chunfei Wang, Guodong Wang, Jun Han
Summary: In this study, a water-soluble polysaccharide (PLP1) was successfully isolated and purified from Pueraria lobata. It was found that PLP1 was composed of specific glycosidic units and exhibited a better free radical-scavenging ability. Moreover, PLP1 effectively protected the liver against acute alcoholic liver disease (ALD) in mice.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ya Su, Yaqian Liu, Xueyan Hu, Yueqi Lu, Jinyuan Zhang, Wenbo Jin, Wang Liu, Yan Shu, Yuen Yee Cheng, Wenfang Li, Yi Nie, Bo Pan, Kedong Song
Summary: A 3D printed scaffold based on carbon dots-curcumin nano-drug release has been developed for drug delivery after breast cancer surgery. The scaffold showed effective inhibition of tumor growth, antibacterial activity, and promotion of wound healing, making it a promising approach for preventing tumor recurrence.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ekaterina Sokolova, Diane Jouanneau, Antonin Chevenier, Murielle Jam, Nathalie Desban, Pierre Colas, Elizabeth Ficko-Blean, Gurvan Michel
Summary: Carrageenans, a compound synthesized in red algae, have various biological properties and are valuable in the pharmaceutical and cosmetic industries. Their fine structure affects wound healing, oxidative processes, hemostasis, and inflammation. Enzymatic modification of carrageenans produces oligosaccharides that bind to natural human serum antibodies and specific antibodies, showing potential for therapeutic applications.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Yan Shi, Miao Yu, Kaijin Qiu, Tiantian Kong, Chunjing Guo, Wenxue Zhang, Daquan Chen, Ming Kong
Summary: In this study, functionalized transfersomes were developed to co-deliver doxorubicin and 1MT towards primary tumors and tumor draining lymph nodes via transdermal administration using microneedles. The results showed that the nano-complexed microneedles exhibited a stronger suppression in tumor growth compared to the intravenous group.
CARBOHYDRATE POLYMERS
(2024)