Article
Gastroenterology & Hepatology
Juhoon So, Minwook Kim, Seung-Hoon Lee, Sungjin Ko, Daniel A. Lee, Hyewon Park, Mizuki Azuma, Michael J. Parsons, David Prober, Donghun Shin
Summary: The differentiation of liver progenitor cells (LPCs) into hepatocytes is crucial for reducing inflammation and fibrosis in chronic liver diseases. Inhibition of the epidermal growth factor receptor (EGFR) signaling pathway promotes LPC-to-hepatocyte differentiation and may serve as a proregenerative therapeutic approach for patients with advanced liver disease. This study provides insights into the mechanisms underlying LPC-mediated liver regeneration and suggests the potential of EGFR inhibitors as a treatment option.
Article
Cell & Tissue Engineering
Sixia Huang, Paola Kuri, Yann Aubert, Megan Brewster, Ning Li, Olivia Farrelly, Gabriella Rice, Hyunjin Bae, Stephen Prouty, Tzvete Dentchev, Wenqin Luo, Brian C. Capell, Panteleimon Rompolas
Summary: Lgr6 epidermal stem cells play a crucial role in wound re-epithelialization and interact with nerves to regulate their fate. Loss of nerves affects the wound closure process, leading to recruitment of other stem cell populations for compensation.
Article
Biotechnology & Applied Microbiology
Martina Fiumara, Samuele Ferrari, Attya Omer-Javed, Stefano Beretta, Luisa Albano, Daniele Canarutto, Angelica Varesi, Chiara Gaddoni, Chiara Brombin, Federica Cugnata, Erika Zonari, Matteo Maria Naldini, Matteo Barcella, Bernhard Gentner, Ivan Merelli, Luigi Naldini
Summary: Base and prime editors have shown potential for precise genetic engineering, but their cellular responses and genotoxicity are not well understood. This study compared the efficiency, toxicity, and genotoxicity between base and prime editors and Cas9 in human hematopoietic cells. The findings suggest that these editors can induce harmful effects and genotoxicity, raising concerns for their clinical applications.
NATURE BIOTECHNOLOGY
(2023)
Article
Oncology
Mengkai Yang, Tao Zhang, Yangfeng Zhang, Xiaojun Ma, Jing Han, Ke Zeng, Yafei Jiang, Zongyi Wang, Zhuoying Wang, Jing Xu, Yingqi Hua, Zhengdong Cai, Wei Sun
Summary: The study revealed that MYLK4 promotes the growth and metastasis of osteosarcoma by activating the EGFR signaling pathway. Additionally, the combination of MYLK4 and EGFR inhibitors showed synergistic effects on the growth and metastasis of OS in vitro and in vivo.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Multidisciplinary Sciences
Ines Paniagua, Zainab Tayeh, Mattia Falcone, Santiago Hernandez Perez, Aurora Cerutti, Jacqueline J. L. Jacobs
Summary: MAD2L2, a member of the shieldin complex, protects stalled replication forks from excessive resection. Its loss leads to uncontrolled DNA resection, causing genomic damage. MAD2L2 functions independently of shieldin and cooperates with REV3L and REV1 to promote fork stability.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Teodora Bavaro, Sara Tengattini, Refaya Rezwan, Enrica Chiesa, Caterina Temporini, Rossella Dorati, Gabriella Massolini, Bice Conti, Daniela Ubiali, Marco Terreni
Summary: This study aimed to develop bioconjugates based on hEGF for tissue engineering applications, with immobilized EGF showing significantly high cell proliferative activity at 50 and 100 ng/mL. The results suggest that EGF immobilized on activated-agarose carriers has potential for treating skin-related disorders such as burns.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Yongjian Huang, Jana Ognjenovic, Deepti Karandur, Kate Miller, Alan Merk, Sriram Subramaniam, John Kuriyan
Summary: Research has shown that the EGFR receptor can adopt different conformations of the extracellular module when binding to different ligands, with implications for intracellular signaling pathways.
Article
Cardiac & Cardiovascular Systems
Wenbin Fu, Hongmei Ren, Jialing Shou, Qiao Liao, Liangpeng Li, Yu Shi, Pedro A. Jose, Chunyu Zeng, Wei Eric Wang
Summary: Long non-coding RNA NPPA-AS1 negatively regulates cardiomyocyte proliferation and its deletion promotes cardiac regeneration in both neonatal and adult mice. NPPA-AS1 deletion improves cardiac function and reduces infarct size, associated with increased cardiomyocyte proliferation. The mechanism involves the interaction of NPPA-AS1 with SFPQ, a DNA repair-related molecule, leading to enhanced DNA repair and activation of cardiomyocyte cell cycle re-entry.
BASIC RESEARCH IN CARDIOLOGY
(2022)
Article
Multidisciplinary Sciences
Brendan Sullivan, Taylor Light, Vinh Vu, Adrian Kapustka, Kalina Hristova, Deborah Leckband
Summary: This study reveals a force-transduction mechanism that connects mechanical perturbations of E-cadherin to the activation of EGFR, a key regulator of cell proliferation. The results demonstrate that E-cadherin and EGFR form complexes at the plasma membrane, which are disrupted by the application of force or the presence of EGF. The findings provide insights into the initial steps of E-cadherin-mediated force transduction and the activation of growth regulatory signaling cascades.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Nanoka Suzuki, Akinori Kanai, Yutaka Suzuki, Hajime Ogino, Haruki Ochi
Summary: The Klf15-dependent adrenergic receptor signaling through regeneration enhancers plays a central role in the genetic network for kidney regeneration in Xenopus laevis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Fanting Zeng, Yuhang Peng, Yuefeng Qin, Jianming Wang, Guoyun Jiang, Wenli Feng, Ying Yuan
Summary: Wee1 plays a crucial role in the treatment of CML by regulating DNA damage repair. Inhibiting Wee1 can improve the sensitivity to Imatinib and inhibit cancer cell proliferation.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biotechnology & Applied Microbiology
Benjamin Atwell, Cheng-Yu Chen, Matthew Christofferson, William R. Montfort, Joyce Schroeder
Summary: Targeting the retrotranslocation of EGFR may be a potent therapeutic approach for RTK-active cancer.
CANCER GENE THERAPY
(2023)
Article
Cardiac & Cardiovascular Systems
Hana Seung, Jan Wrobel, Carolin Wadle, Timon Buehler, Diana Chiang, Jasmin Rettkowski, Nina Cabezas-Wallscheid, Beatrice Hechler, Peter Stachon, Alexander Maier, Christian Weber, Dennis Wolf, Daniel Duerschmied, Marco Idzko, Christoph Bode, Constantin von zur Muehlen, Ingo Hilgendorf, Timo Heidt
Summary: This study investigates the role of adenosine diphosphate (ADP)-dependent P2Y(12)-mediated signaling in emergency hematopoiesis and cardiac remodeling after myocardial infarction (MI). The results show that ADP acts as a danger signal, promoting emergency hematopoiesis through Akt phosphorylation and cell cycle progression. Inhibiting P2Y(12) can reduce emergency hematopoiesis, excessive inflammatory response, and improve cardiac function after MI.
BASIC RESEARCH IN CARDIOLOGY
(2022)
Article
Cell Biology
Rebecca J. Burgess, Zhiyu Zhao, Daisuke Nakada, Sean J. Morrison
Summary: The study showed that deficiency of Bmi1 leads to increased division of HSCs without inducing senescence. Bmi1 promotes HSC quiescence by negatively regulating ARX expression and promoting proteostasis by suppressing protein synthesis.
GENES & DEVELOPMENT
(2022)
Review
Cell Biology
Jingxin Zhang, Binjing Zhang, Jinglan Zhang, Weimin Lin, Shiwen Zhang
Summary: Magnesium as a suitable material for promoting peripheral nerve regeneration has both challenges and potentials, but there is currently limited research on this topic, requiring further exploration to address the shortcomings and challenges identified.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Heather A. Himburg, Martina Roos, Tiancheng Fang, Yurun Zhang, Christina M. Termini, Lauren Schlussel, Mindy Kim, Amara Pang, Jenny Kan, Liman Zhao, Hyung Suh, Joshua P. Sasine, Gopal Sapparapu, Peter M. Bowers, Gary Schiller, John P. Chute
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Article
Hematology
Christina M. Termini, Amara Pang, Destiny M. Batton, John P. Chute
Summary: PTPo- is highly expressed by HSCs and negatively regulates their self-renewal and regeneration. Syndecan-2 promotes PTPo- clustering, leading to increased levels of phospho-tyrosine and phospho-ezrin in HSPCs and enhancing hematopoietic colony formation. Strategies that promote PTPo- clustering on HSPCs may have the potential to significantly boost hematopoietic function.
EXPERIMENTAL HEMATOLOGY
(2021)
Article
Hematology
Christina M. Termini, Amara Pang, Michelle Li, Tiancheng Fang, Vivian Y. Chang, John P. Chute
Summary: The discovery of Syndecan-2 as a novel surface marker and regulator of hematopoietic stem cell (HSC) function has improved the understanding of HSC identity and their capacity for repopulation. Syndecan-2 expression is significantly increased in long-term HSCs compared to differentiated hematopoietic cells. Isolation of bone marrow cells based on Syndecan-2 expression results in a higher enrichment of HSCs with superior in vivo repopulating capacity, compared to conventional selection methods. Syndecan-2 regulates HSC repopulating capacity through control of Cdkn1c expression and HSC quiescence.
Article
Multidisciplinary Sciences
Christina M. Termini, Amara Pang, Tiancheng Fang, Martina Roos, Vivian Y. Chang, Yurun Zhang, Nicollette J. Setiawan, Lia Signaevskaia, Michelle Li, Mindy M. Kim, Orel Tabibi, Paulina K. Lin, Joshua P. Sasine, Avradip Chatterjee, Ramachandran Murali, Heather A. Himburg, John P. Chute
Summary: Ionizing radiation and chemotherapy deplete hematopoietic stem cells and damage the vascular niche, leading to the secretion of SEMA3A from bone marrow endothelial cells. Inhibition of NRP1 promotes vascular and hematopoietic regeneration through increased expression of R spondin 2, suggesting a potential therapeutic target for improving hematopoietic recovery after myeloablation.
NATURE COMMUNICATIONS
(2021)
Editorial Material
Biochemical Research Methods
John P. Chute, Joshua P. Sasine
Summary: Image-seq combines high-resolution microscopy with single-cell transcriptomics to spatially resolve and isolate viable bone marrow and leukemia cells, achieving a long-sought integration of single-cell molecular profiling and cellular spatial localization.
Editorial Material
Cell Biology
Betsabel Chicana, Christina Marie Termini
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)
Editorial Material
Hematology
John P. Chute
Summary: This study demonstrates that aging and DNA repair gene deficiency can lead to upregulation and secretion of AREG by bone marrow stromal cells, resulting in a decline in hematopoietic stem cell repopulating capacity.