Spectroscopic analysis of Muga silk nanoparticles synthesized by microwave method

Muga silk nanoparticles (MSNP) were synthesized using a microwave-assisted radiolysis method. The effect of microwave on the Muga protein secondary structures was analyzed. The evolution of the secondary structure from random coils to the beta-sheets was determined by using FTIR, circular dichroism and X-ray diffraction techniques. The results showed that Muga silk fibroin protein contained the primary structure in silk-I state. When the protein was irradiated with microwave, nanoparticle synthesis was possible having silk-II state imparting crystallinity. The silk nanoparticles were characterized by a particle size analyzer and found to be of similar to 240 nm in size. The optical properties of these nanoparticles were studied by UV-vis. spectroscopy and photoluminescence. For studying thermal properties, differential scanning calorimetry was performed that revealed early glass transition, which could be attributed to the presence of water and proteins. It also revealed that nanoparticles are thermally stable. Such studies are important for understanding more about the MSNP and would be beneficial for their further wide applications. (c) 2020 International Union of Biochemistry and Molecular Biology, Inc.

Automated summary

Muga silk nanoparticles were synthesized using microwave-assisted radiolysis method, leading to a shift in the silk protein secondary structures. The nanoparticles exhibited silk-II state and thermal stability, indicating potential for wide applications. These studies provide important insights into the characteristics of MSNP for future research and applications.