期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 112, 期 10, 页码 2051-2059出版社
WILEY
DOI: 10.1002/bit.25624
关键词
microbial fuel cells; bioelectrochemical systems; biofilm; Shewanella; c-di-GMP; extracellular electron transfer
资金
- Ministry of Science and Technology of China [2012AA02A701, 2014CB745100]
- National Natural Science Foundation of China [21376174]
- Ministry of Education, Singapore [MOE2011-T2-2-035]
- Environment and Water Industry (EWI) Programme Office of Singapore [1102-IRIS-02-03]
- National Research Foundation, Singapore [NRF-CRP 001-032]
- AcRF [RG 78/10]
Electroactive biofilms play essential roles in determining the power output of microbial fuel cells (MFCs). To engineer the electroacitve biofilm formation of Shewanella oneidensis MR-1, a model exoelectrogen, we herein heterologously overexpressed a c-di-GMP biosynthesis gene ydeH in S. oneidensis MR-1, constructing a mutant strain in which the expression of ydeH is under the control of IPTG-inducible promoter, and a strain in which ydeH is under the control of a constitutive promoter. Such engineered Shewanella strains had significantly enhanced biofilm formation and bioelectricity generation. The MFCs inoculated with these engineered strains accomplished a maximum power density of 167.6 +/- 3.6mW/m(2), which was approximate to 2.8 times of that achieved by the wild-type MR-1 (61.0 +/- 1.9mW/m(2)). In addition, the engineered strains in the bioelectrochemical system at poised potential of 0.2V vs. saturated calomel electrode (SCE) generated a stable current density of 1100mA/m(2), approximate to 3.4 times of that by wild-type MR-1 (320mA/m(2)). Biotechnol. Bioeng. 2015;112: 2051-2059. (c) 2015 Wiley Periodicals, Inc.
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