期刊
BIOELECTROCHEMISTRY
卷 132, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2019.107418
关键词
Biosensor; Laccase; Tannic acid; Magnetic nanoparticles; Enzyme immobilization; Phenolic determination; Hybrid nanomaterials
资金
- Veneto Region project Safe, Smart, Sustainable Food for Health (3S_4H)-POR FESR 20142020, 1.1.4. DGR [1139]
- Cariparo Foundation (Padova and Rovigo, Italy)
- DOR funds of the University of Padova [DORI 989373]
- Italian Ministry of Education, University and research (MIUR) Excellence for Aquatic Animal Health -ECCE AQUA
A shell of nanostructured ferric tannates was spontaneously developed on the surface of naked maghemite nanoparticles (SAMNs, the core) by a simple wet reaction with tannic acid (TA). The as obtained core-shell nanomaterial (SAMN@TA) displays specific electrocatalytic and surface properties, which significantly differ from parent maghemite. Thanks to the known proclivity of TA to interact with proteins, SAMN@TA was proposed as a support for the direct immobilization of an enzyme. A ternary functional nanobioconjugate (SAMN@TA@TvL) was successfully self-assembled by incubating laccase from Trametes versicolor (TvL) and SAMN@TA. The SAMN@TA@TvL hybrid was kinetically characterized with respect to the native enzyme and applied for building an easy-to-use analytical device for the detection of polyphenols. The electrochemical biosensor allowed the determination of polyphenols by square wave voltammetry in mixed water-methanol solutions. The system sensitivity was 868.9 +/- 1.9nA mu M-1, the LOD was 81 nM and the linearity range was comprised between 100 nM and 10 mu M. The proposed approach was successfully applied to detect phenolics in blueberry extracts as real samples. Results suggest that SAMN@TA could be a promising, low cost and versatile tool for the creation of nano-bioconjugates aimed at the development of new electrochemical sensing platforms. (C) 2019 Elsevier B.V. All rights reserved.
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