期刊
BIOELECTROCHEMISTRY
卷 132, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2019.107452
关键词
Carcinoembryonic antigen; Electrochemical immunosensor; Graphene nanoplatelets; Manganese dioxide; Core shell Fe3O4@Au nanoparticles
资金
- National Research Council of Thailand
- Department of Chemistry, Faculty of Science, Ubon Ratchathani University (Thailand)
- Institute of Chemistry-Analytical Chemistry, Karl Franzens University (Austria)
- Center of Excellence for Innovation in Chemistry (PERCH-CIC), Office of the Higher Education Commission, Ministry of Education (OHEC)
- Thailand Graduate Institute of Science and Technology (TGIST) [TG-55-24-60-010D]
- fellowship INNOVASIA from the Pays de la Loire Region, France
- ASEAN European Academic University Network (ASEA-UNINET)
- Bernd Rode Award 2019
A novel electrochemical immunosensor was developed for label-free detection of carcinoembryonic antigen (CEA) as a cancer biomarker. The designed immunosensor was based on CEA antibody (anti-CEA) anchored with core shell Fe3O4@Au nanoparticles which were immobilized on a screen-printed carbon electrode modified with manganese dioxide decorating on graphene nanoplatelets (SPCE/GNP-MnO2/Fe3O4@Au-antiCEA). The SPCE was placed onto a home-made electrode holder for easy handling. The approach was based on direct binding of CEA to a fixed amount of anti-CEA on the modified electrode for the specific detection using linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS) monitored in a solution containing 5 mM [Fe(CN)(6)(3-/4-)] prepared in 0.1 M phosphate buffer at pH 7.4. The difference in signal response owing to the redox reaction of [Fe(CN)(6)](3-/4-) before and after interaction with CEA was regarded as the immunosensor response corresponding directly to the CEA concentration. Under optimized conditions, the linear range of 0.001-100 ng/mL, and the detection limits of 0.10 pg/mL (LSV) and 0.30 pg/mL (EIS) were evaluated. The applicability of the immunosensor was verified by well-corresponding determination of CEA in diluted human serum samples by electrochemiluminescence (ECL) immunoassay. Therefore, the proposed immunosensor could be suitable enough for a real sample analysis of CEA. (C) 2020 Elsevier B.V. All rights reserved.
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