Article
Obstetrics & Gynecology
Jun Tan, Pei-Pei Liu, Li-Yun Cao, Yang Zou, Zi-Yu Zhang, Jia-Lyu Huang, Zhi-Qin Zhang, Ding-Fei Xu, Lu Fan, Lei-Zhen Xia, Qi Xie, Li-Feng Tian, Cai-Lin Xin, Zeng-Ming Li, Qiong-Fang Wu
Summary: This study investigated the regulatory mechanisms of abnormal follicular development in PCOS patients. The researchers found that the proliferation ability of ovarian granulosa cells (GCs) was reduced in PCOS patients. Furthermore, the expression of PATL2 and ADM2 was decreased in the GCs of PCOS patients. Knocking down PATL2 led to impaired GC growth and weakened ADM2 expression. The findings suggest that PATL2 promotes GC proliferation by stabilizing ADM2 expression, and the reduction of PATL2 in polycystic ovaries may result in aberrant follicles.
REPRODUCTIVE SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Bi Yu-lin, Yang Shu-yan, Wang Hai-yan, Chang Guo-bin, Chen Guo-hong
Summary: This study confirmed the expression of the FSH gene in chicken follicles and its role in promoting ovarian granulosa cell proliferation, enriching the theory on the HPG axis.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Immunology
Reza Salehi, Meshach Asare-Werehene, Brandon A. Wyse, Atefeh Abedini, Bo Pan, Alex Gutsol, Sahar Jahangiri, Peter Szaraz, Kevin D. Burns, Barbara Vanderhyden, Julang Li, Dylan Burger, Clifford L. Librach, Benjamin K. Tsang
Summary: Polycystic ovarian syndrome (PCOS) is associated with increased levels of androgen and arrested follicle growth. Previous studies have shown that androgen-induced release of miR-379-5p from follicle granulosa cells promotes cell proliferation through PDK1 upregulation. Androgen also affects macrophage polarization and increases the inflammatory M1 phenotype in rat follicles. However, the role of small extracellular vesicles (exosomes) in mediating the effects of miRNAs on receiving cells is not well understood. This study aimed to investigate the regulatory role of exosomal miR-379 from granulosa cells on macrophage polarization and ovarian inflammation, and its impact on granulosa cell proliferation in a stage-specific manner.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jing Luo, Hong Ye, Lijuan Hao, Yixuan Sun, Ruohan Li, Yanxi Li, Zhu Yang
Summary: The study identified the presence of AR alternative splicing variants AR-SVs in PCOS patients' GCs, leading to reduced AR function. miRNA-183 and miRNA-124 were found to regulate the expression of SRSF2 and SRSF3, influencing AR function and androgen accumulation in the ovary.
Article
Agriculture, Dairy & Animal Science
Chunyang Niu, Sujuan Zhang, Guilin Mo, Yilong Jiang, Liang Li, Hengyong Xu, Chunchun Han, Hua Zhao, Yanhong Yan, Shenqiang Hu, Jiwei Hu, Bo Kang, Dongmei Jiang
Summary: In this study, we successfully induced ODC interference and overexpression in goose ovarian GCs, mainly affecting putrescine content with slight influence on spermidine and spermine. Additionally, ODC participated in regulating P4 levels in the culture medium of GCs, promoting granulosa cell proliferation, and inhibiting granulosa cell apoptosis.
Article
Biotechnology & Applied Microbiology
Yingying Yu, Guojing Li, Xiaoying He, Yu Lin, Zheng Chen, Xianhua Lin, Hong Xu
Summary: This study demonstrated that miR-21 and TLR8 were significantly upregulated in PCOS granulosa cells, leading to increased inflammatory response. These findings provide important insights into the pathogenesis of PCOS.
Article
Genetics & Heredity
Liping Zou, Qiwen Feng, Wei Xia, Changhong Zhu
Summary: This study aimed to identify common targets of miR-223-3p, miR-122-5p, and miR-93-5p in PCOS and assess their transcript levels in PCOS rat ovaries. Transcriptome profiles of granulosa cells from PCOS patients were analyzed to identify DEGs, followed by miRNA target prediction and functional analysis. The study identified several potential genes, including JMJD1C, PLCG2, SMAD3, FOSL2, TGFB1, TRIB1, GAS7, TRIM25, NFYA, and CALCRL, which may play a role in the development of PCOS. These findings contribute to the identification of potential biomarkers for the prevention and treatment of PCOS in the future.
FRONTIERS IN GENETICS
(2023)
Article
Biology
Xiaohong Chen, Hengzhen He, Bingcai Long, Binli Wei, Peng Yang, Xiaoying Huang, Qian Wang, Jing Lin, Hongliang Tang
Summary: This study found that acupuncture can inhibit granulosa cell apoptosis and improve follicular development abnormalities in patients with PCOS by targeting and regulating miR-21-3p through LncMEG3. These findings highlight the clinical potential of acupuncture as a safe treatment for follicular developmental abnormalities in PCOS.
BIOLOGICAL RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Liang Huang, Huan Yuan, Shengjie Shi, Xiangrong Song, Lutong Zhang, Xiaoge Zhou, Lei Gao, Weijun Pang, Gongshe Yang, Guiyan Chu
Summary: The CLOCK protein was found to inhibit the proliferation of porcine ovarian granulosa cells by increasing ASB9 level.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Medicine, General & Internal
Yanjun Zheng, Yuehong Bian, Richao Wu, Wei Chen, Linlin Fu, Ping Li, Ying Wang, Xiao Yang, Shigang Zhao, Yuhua Shi
Summary: This study revealed that lncRNAs and mRNAs in GCs are involved in the occurrence and development of PCOS, contributing to clarify the pathogenesis mechanism of PCOS.
FRONTIERS IN MEDICINE
(2021)
Article
Oncology
Min Wang, Yixuan Sun, Dong Yuan, Song Yue, Zhu Yang
Summary: This study found that exosomal miR-4449 derived from follicular fluid was significantly decreased in women with PCOS and that it could alleviate oxidative stress and promote proliferation in granulosa cells. Additionally, miR-4449 was shown to regulate the KEAP1/NRF2 signaling pathway, affecting granulosa cell proliferation and oxidative stress. These findings contribute to a new understanding of the pathogenesis of PCOS.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Endocrinology & Metabolism
Yi-xuan Wu, Yan-shan Lin, Si-chen Li, Xi Yao, Mingwei Cheng, Lin Zhu, Hai-ying Liu
Summary: The study revealed that miR-194 is upregulated in PCOS, affecting cell growth, proliferation, and apoptosis in KGN cells. Additionally, the pro-apoptotic effects of miR-194 overexpression can be rescued by upregulating HB-EGF expression.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Veterinary Sciences
A. Tarko, A. Stochmalova, A. H. Harrath, J. Kotwica, A. Balazi, A. Sirotkin
Summary: This study examined the effects of the plant polyphenol quercetin and the environmental contaminant toluene on basic ovarian cell functions, and found that quercetin has a protective effect against the adverse effects of toluene. Toluene stimulated cell proliferation and inhibited progesterone, IGF-I, and testosterone release, while quercetin inhibited cell proliferation, apoptosis, IGF-I, and testosterone release and stimulated progesterone output. When administered together, quercetin mitigated toluene's effects on proliferation and progesterone release and induced toluene to exhibit a pro-apoptotic effect.
RESEARCH IN VETERINARY SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Chen-Hua Zhang, Xiang-Yi Liu, Jing Wang
Summary: Granulosa cells play a crucial role in facilitating communication with oocytes through various material exchange modes. Glucose and lipid metabolism pathways in granulosa cells are important for oocyte development. Changes in intrafollicular hormone levels are regulated by granulosa cells through processing of steroid hormones, controlling the development process of oocytes. The study also explores the effects of glucose and lipid metabolism on oocytes under pathological conditions, particularly in relation to polycystic ovary syndrome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Ying Wang, Jiyu Yang, Yu Wang, Yao Chen, Yiran Wang, Hongying Kuang, Xiaoling Feng
Summary: In this study, we investigated the role and mechanism of TXNIP in PCOS. TXNIP was found to be upregulated in the ovaries of PCOS mice and testosterone-treated granulosa cells. Knockdown of TXNIP attenuated GC injury, oxidative stress, inflammation, and NLRP3 inflammasome activation. Furthermore, knockdown of TXNIP ameliorated ovarian injury and inflammation in mice with DHEA-induced PCOS. These findings suggest that TXNIP is involved in GC inflammation by promoting NLRP3 inflammasome activation in PCOS.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2023)