Article
Biochemistry & Molecular Biology
Fei Li, Xu Jiao, Jing Zhao, Xiaojun Liao, Yunlu Wei, Quanhong Li
Summary: YL-11 EPS has shown the ability to inhibit colon cancer cell proliferation, with in vivo studies confirming its anti-cancer effects and in vitro experiments demonstrating its involvement in regulating mitochondrial pathways and PI3K/AKT signaling pathways.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Nutrition & Dietetics
Shumei Wang, Yi Shan, Shuang Zhang, Lanwei Zhang, Yuehua Jiao, Dijia Xue, Lili Zhang, Huaxi Yi
Summary: In this study, it was found that whole peptidoglycan (WPG) from L. paracasei subsp. paracasei X12, obtained from traditional cheese in northwestern China, inhibited proliferation and induced apoptosis in HT-29 cells in a dose-dependent manner. The induction of apoptosis by WPG was associated with mitochondrial dysfunction and ROS accumulation, as well as the activation of Caspase 3 and alterations in the expression of proapoptotic and antiapoptotic genes. These findings suggest that WPG could potentially be used as a natural agent for cancer treatment.
Article
Cell Biology
Dasa Cizkova, Milan Cizek, Marcela Maloveska, Marta Kmetova, Vladimir Kmet, Dobroslava Bujnakova
Summary: The findings suggest that cell-free L. casei 21L10 supernatant may have potential in attenuating LPS-induced inflammation, inhibiting proliferation, and promoting apoptosis/necrosis. While this study provides insight into using L. casei exoproducts as anti-inflammatory or anti-proliferative agents for gut diseases, further data is required to confirm their efficacy.
Review
Biochemistry & Molecular Biology
Evita Balducci, Francesco Papi, Daniela Eloisa Capialbi, Linda Del Bino
Summary: Bacteria and fungi have developed resistance to antibiotics and antifungal drugs by forming biofilms. Biofilms allow the transfer of resistance genes, prevent desiccation, and impede the penetration of drugs. Biofilms consist of extracellular DNA, proteins, and polysaccharides. Different polysaccharides play roles in attachment and structure resistance of biofilms. This review discusses the structure and role of polysaccharides in biofilms and summarizes potential therapies targeting exopolysaccharides to inhibit biofilm formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Environmental
N. M. Pfaff, J. A. Dijksman, A. J. B. Kemperman, M. C. M. van Loosdrecht, J. M. Kleijn
Summary: The mechanical properties of bacterial alginate-like exopolymers (ALE) gels were studied to understand their role in biofilm resilience to cleaning efforts. Increasing CaCO3 concentration in the gels led to higher elastic modulus but also increased brittleness. Proper dosing of Ca salts may be an effective approach to remove biofouling.
Review
Biochemistry & Molecular Biology
Jonathan Chung, Shafinaz Eisha, Subin Park, Amanda J. Morris, Isaac Martin
Summary: In cystic fibrosis (CF), pulmonary infection with Pseudomonas aeruginosa is a major cause of morbidity and mortality. The biofilm mode of growth of P. aeruginosa, with its self-secreted exopolysaccharides (EPSs), provides physical protection against antibiotics and leads to phenotypic heterogeneity. Current antimicrobials are inadequate in eradicating P. aeruginosa infection, thus there is a need for new therapies. This review focuses on the investigation and exploitation of the three biofilm-associated EPSs as potential therapeutic targets for combating pulmonary infection with P. aeruginosa in CF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Jun Bao, Ziyu Jiang, Wenlong Ding, Yuepeng Cao, Liu Yang, Jingbing Liu
Summary: This study synthesized AgNPs using gallic acid as a reductant and characterized them using various techniques. The synthesized AgNPs exhibited cytotoxicity on colorectal adenocarcinoma cells (HT-29) and induced apoptosis and non-canonical autophagy. Further research using animal models is needed to understand the exact mechanisms of AgNPs in the treatment of digestive tract neoplasms.
NANOTECHNOLOGY REVIEWS
(2022)
Article
Immunology
Qi Lili, Lu Xiaohui, Mao Haiguang, Wang Jinbo
Summary: C. butyricum can adhere to the mucus secreted by HT-29 cells with the glycan playing an important role in this process. Knockdown experiments targeting MUC2, FUT2, or GALNT7 significantly reduced the adhesion of bacteria to HT-29 cells. When colonizing on the surface of HT-29 cells, C. butyricum can increase mucin production and induce glycosylation, indicating its ability to modulate the glycosylation profile of mucus secreted by HT-29 cells.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Ahmed Zayed, Mai K. Mansour, Mohamed S. Sedeek, Mohamed H. Habib, Roland Ulber, Mohamed A. Farag
Summary: Bacteria form biofilms through extracellular polymeric substances (EPS) and their production is influenced by various factors. EPS derived from marine and terrestrial bacteria have diverse applications but are not yet commercially implemented.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Qiuxia Fan, Yahong Yuan, Hang Jia, Xuejun Zeng, Zhouli Wang, Zhongqiu Hu, Zhenpeng Gao, Tianli Yue
Summary: The study showed that thymoquinone has antimicrobial and anti-biofilm activities against Shigella flexneri by disrupting cell morphology, inhibiting protein synthesis, and downregulating genes involved in biofilm formation. Thymoquinone has the potential to be used as a natural agent to combat contamination and infection caused by Shigella flexneri.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Microbiology
Zaira Heredia-Ponce, Antonio de Vicente, Francisco M. Cazorla, Jose Antonio Gutierrez-Barranquero
Summary: Biofilm formation is a result of a multicellular growth mode where bacteria are encased in their own extracellular matrix. Pseudomonas genus bacteria, especially Pseudomonas aeruginosa, have been extensively studied due to their metabolic versatility and ecological significance. The extracellular matrix of P. aeruginosa is primarily composed of exopolysaccharides, such as alginate and Psl polysaccharides, which play crucial roles in biofilm architecture and pathogenic features.
Article
Polymer Science
Naser Rasouli, Daryoush Shahbazi-Gahrouei, Simin Hematti, Behzad Baradaran, Roya Salehi, Jaleh Varshosaz, Abbas Jafarizad
Summary: This study investigates the potential of oxaliplatin-loaded iodine nanoparticles in the treatment of colorectal cancer. The results show that these nanoparticles can enhance the radiosensitivity and promote apoptosis of cancer cells. Additionally, the nanoparticles have no impact on cell cycle development. Therefore, iodine nanoparticles may be used as a treatment for colorectal cancer.
Article
Microbiology
Yingxue Yue, Song Wang, Jialu Shi, Qinggang Xie, Na Li, Jiaqi Guan, Smith Etareri Evivie, Fei Liu, Bailiang Li, Guicheng Huo
Summary: This study investigates the mechanism of action of Lactobacillus acidophilus KLDS1.0901 on apoptosis in colon cancer cells. The results demonstrate that L. acidophilus KLDS1.0901 can inhibit the proliferation of HT-29 and Caco-2 cells and induce cell apoptosis. Transcriptomics research reveals that L. acidophilus KLDS1.0901 may be involved in the apoptosis process through the nuclear factor kappa B and PI3K-AKT signaling pathways.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Applied
Yurong Li, Shaocong Wang, Zhen Xing, Yiming Niu, Zhencheng Liao, Yang Lu, Junni Qiu, Junfeng Zhang, Chunming Wang, Lei Dong
Summary: Eliminating biofilms from infected tissue is a challenging task in treating chronic wounds. The study found that cationic polymers can disrupt biofilms and enhance bacterial antibiotic sensitivity. In a mouse wound healing model, cationic polymers effectively controlled infection and accelerated the healing process.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Multidisciplinary
Yeeun Kim, Hak Jun Kim, Keunho Ji
Summary: Postbiotics, the next-generation compounds, have gained attention for their potential health benefits. This study investigated the anti-proliferation effects of postbiotics from Lactobacillus strains on colon cancer cells. The results showed that the postbiotics demonstrated a concentration-dependent anti-proliferation effect, making them promising compounds for anticancer research.
APPLIED SCIENCES-BASEL
(2022)