Article
Chemistry, Analytical
Sotaro Takiguchi, Fumika Kambara, Mika Tani, Tsuyoshi Sugiura, Ryuji Kawano
Summary: This paper presents a strategy for simultaneous recognition of over- and under-expressed microRNAs (miRNAs) using signal classification-based nanopore decoding. By designing diagnostic DNA probes and analyzing the characteristic current signals obtained through nanopore measurements, the study successfully achieved pattern recognition of over/under-expressed miRNAs, providing a potential method for miRNA-targeting simple diagnosis as a liquid biopsy.
ANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Songlin He, Ruiming Cui, Yao Zhang, Yongkang Yang, Ziheng Xu, Shuoyu Wang, Pingxiu Dang, Kexin Dang, Qing Ye, Yin Liu
Summary: Researchers have proposed a more stable design of logic gates based on T-dsDNA structure. They have demonstrated computations using these DNA nanocircuits in a microfluidic chip device at room temperature and obtained expected real-time results.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Xiaokang Zhang, Yuan Liu, Bin Wang, Shihua Zhou, Peijun Shi, Ben Cao, Yanfen Zheng, Qiang Zhang, Kirilov Kasabov Nikola
Summary: The rise of DNA nanotechnology is driving the development of molecular security devices and bringing about important changes in information security technology. However, developing a secure and reliable access control strategy remains a challenge.
Review
Chemistry, Analytical
Mengyao Cao, Xiewei Xiong, Yun Zhu, Mingshu Xiao, Li Li, Hao Pei
Summary: DNA computing has made rapid progress in the past decade. DNA computational devices can perform various powerful computational functions based on DNA functional motifs. They have shown great potential in biosensing applications due to the inherent properties of DNA.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Zimu Li, Min Zhu, Fang Li, Zhi Li, Anqi Zhao, Mohammad A. Haghighatbin, Hua Cui
Summary: This study presents a multicolor chemiluminescence sensing strategy for the discrimination of five antioxidants. By fabricating multilayer paper chips and utilizing chemiluminescence reactions, unique R, G, and B values were generated in the chemiluminescence images for antioxidant discrimination. The experimental results demonstrate the accuracy and feasibility of this strategy in identifying antioxidants.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Zhaohui Qin, Yu Liu, Linghao Zhang, Jiajia Liu, Xin Su
Summary: This study demonstrates a DNA-based artificial dissipative system with a molecular timer, which allows for dynamic enzyme catalysis and DNA nanostructure disassembly. This provides a useful tool for the development of artificial reaction networks and intelligent nanostructures.
Article
Biochemical Research Methods
Tracy L. Mallette, Matthew R. Lakin
Summary: This study presents a novel approach in heterochiral DNA nanotechnology that uses a combination of D-DNA and L-DNA nucleotides in triblock heterochiral copolymers for constructing nanoscale devices in the intracellular environment. The researchers demonstrate that the addition of L-DNA domains as protective caps on D-DNA domains in strand displacement-based molecular circuits improves their resistance to degradation by exonucleases during extended incubations in serum-supplemented media. This advancement in heterochiral circuit design could have practical applications in in vivo biomedical diagnostics.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Mathematical & Computational Biology
Yue Wang, Luhui Wang, Wenxiao Hu, Mengyao Qian, Yafei Dong
Summary: DNA synthesis offers possibilities for designing multifunctional nanodevices, and spatially organized DNA reaction networks can provide a different strategy for molecular circuit synthesis. The Visual DSD modeling language is used to design and analyze this network, allowing for autonomous movement and addressing operations of DNA nanodevices.
INTERDISCIPLINARY SCIENCES-COMPUTATIONAL LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Hao Liu, Fan Hong, Francesca Smith, John Goertz, Thomas Ouldridge, Molly M. Stevens, Hao Yan, Petr Sulc
Summary: Strand displacement is a widely used mechanism in nucleic acid nanotechnology, with toehold length, placement, and mismatches influencing reaction kinetics. Experimental studies show that longer toehold lengths and placement at the 5' end of the substrate accelerate the reaction, while mismatches closer to the toehold-duplex interface slow it down. The comparison of RNA and DNA displacement, as well as hybrid displacement, suggests a role of thermodynamic stabilities and helix rearrangements in the kinetics.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Chemistry, Physical
Yinzhu Lu, Bingqian Lin, Weizhi Liu, Jialu Zhang, Lin Zhu, Chaoyong Yang, Yanling Song
Summary: A modular platform is developed for sequential isolation of tumor and non-tumor extracellular vesicles (EVs) using dual-aptamer recognition and tandem microchips. This method provides new clues for assessing immune heterogeneity and can be used for EV proteome profiling.
Article
Chemistry, Analytical
Zhiguang Wang, Ruipeng Chen, Yue Hou, Yingkai Qin, Shuang Li, Shiping Yang, Zhixian Gao
Summary: In this study, a portable and sensitive method for detecting melamine using stimuli-responsive DNA hydrogels and microfluidic chips was developed. The method utilized melamine aptamer and DNA hydrogel-coated AuNPs for colorimetric detection and quantitative analysis. The combination of microfluidic chips and DNA hydrogels enabled quantitative detection of melamine by taking photos and analyzing the gray value.
ANALYTICA CHIMICA ACTA
(2022)
Article
Engineering, Biomedical
Chenguang Wang, Yi Xu, Shuainan Li, Yi Zhou, Qiuling Qian, Yifan Liu, Xianqiang Mi
Summary: Circulating tumor cells (CTCs) are biomarkers widely used for the early detection, diagnosis, and therapy monitoring of cancer. However, due to their rarity in peripheral blood, isolating and analyzing CTCs with high efficiency, purity, and viability has been a significant technological challenge. In this study, the researchers developed a microfluidic system combining tetrahedral DNA framework, herringbone channel chip, and aptamer-triggered hybridization chain reaction for efficient capture and release of simulated CTCs.
MATERIALS TODAY BIO
(2022)
Article
Biochemical Research Methods
Fanghao Shi, Fei Jia, Zewen Wei, Yan Ma, Zhiguo Fang, Weikai Zhang, Zhiyuan Hu
Summary: The newly developed integrated microfluidic chip allows sequential enrichment, isolation, and characterization of CTCs at single-cell level, with single CTC lysis achieved on the same chip. This approach provides a solid foundation for the analysis of RNA expression profiling of single-cell CTCs.
Review
Engineering, Biomedical
Minh-Chau N. Le, Z. Hugh Fan
Summary: Exosomes play a crucial role in cell-to-cell communication and contain valuable information about biological processes. Microfluidic devices have emerged as superior alternatives for exosome isolation due to the limitations of conventional methods.
BIOMEDICAL MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Cuizheng Zhang, Victoria E. Paluzzi, Ruojie Sha, Natasha Jonoska, Chengde Mao
Summary: This article demonstrates the feasibility of implementing logic gates, the basic computation operations, in large ensembles of engineered 3D DNA crystals. The outputs are observed through the formation of macroscopic crystals, pointing to a new direction for constructing complex 3D crystal architectures and DNA-based biosensors with easy readouts.
ADVANCED MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Shaokun Wang, Fan Zhang, Qing Yang, Minjing Li, Xun Hou, Feng Chen
Summary: This study proposes a method of fabricating glass infrared artificial compound eyes (ACE) using femtosecond laser wet etching and precision glass molding. The fabricated glass infrared ACE demonstrates high resolution, large field of view, and low aberration. Additionally, the proposed technology offers advantages of low cost and high efficiency.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Chemistry, Multidisciplinary
Qiao Zhang, Kai Xia, Meng Jiang, Qingting Li, Weigang Chen, Mingzhe Han, Wei Li, Rongqin Ke, Fei Wang, Yongxing Zhao, Yuehua Liu, Chunhai Fan, Hongzhou Gu
Summary: This study presents a new method called PECAN (paired-end cutting assisted by DNAzymes), which allows for the mass production of high-quality single-stranded DNA with arbitrary sequences. The method utilizes self-hydrolyzing DNAzymes to generate precursor DNA, which can then be amplified and processed to release large quantities of target DNA. PECAN offers high customizability, purity, and scalability, making it suitable for a wide range of applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Xiaowen Cao, Feng Chen, Jing Xue, Yue Zhao, Min Bai, Yongxi Zhao
Summary: Here, we present a hierarchical DNA branch assembly-encoded fluorescent nanoladders for denoised and highly multiplexed signal amplification of single-cell transcripts. Our method achieves independent RNA-primed rolling circle amplification without nonspecific amplification and allows for programmable DNA branch assembly to encode virtual signals for visualizing numbers of targets. We demonstrate accurate RNA profiling in different cancer cells and reveal diverse localization patterns for spatial regulation of transcripts.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Materials Science, Multidisciplinary
Jie Liang, Hao Wang, Tao Hu, Chao Shan, Qing Yang, Chengjun Zhang, Tongzhen Yang, Zheng Fang, Xun Hou, Feng Chen
Summary: Fog can severely damage optical systems and reduce the imaging quality of optical components. Traditional methods have limitations, but a novel method using femtosecond lasers to fabricate anti-fogging surfaces on silica glass shows promise for durable and environment-friendly optical devices with real-time anti-fogging capabilities.
ADVANCED OPTICAL MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Mengmeng Liu, Xiaoyu Zhang, Lulu Huang, Jie Li, Chunhai Fan, Yang Tian
Summary: This article reviews the evolution of DNA nanostructure-guided plasmon coupling architectures, including the introduction of DNA nanostructures and DNA modification on the surface of plasmonic nanoparticles.
Article
Chemistry, Multidisciplinary
Dandan Li, Jiang Li, Jiao Hu, Mingjie Tang, Peng Xiu, Yunchang Guo, Tunan Chen, Ning Mu, Lihua Wang, Xuehua Zhang, Guizhao Liang, Huabin Wang, Chunhai Fan
Summary: Understanding how A beta 42 oligomers affect neurons from a mechanobiological perspective is crucial for studying neurodegenerative diseases. Using atomic force microscopy (AFM), we quantitatively investigate the nanomechanical properties of neurons exposed to A beta 42 oligomers. We establish a method called heterogeneity-load-unload nano mechanics (HLUN) which allows us to comprehensively profile the mechanical properties of living neurons and correlate them with biological effects.
Article
Chemistry, Multidisciplinary
Lulu Huang, Xiuhai Mao, Jie Li, Qian Li, Jianlei Shen, Mengmeng Liu, Chunhai Fan, Yang Tian
Summary: By designing dual-emissive gold nanoparticles with varying spikiness, the interactions between spiky nanoparticles and cells were investigated. It was found that the nanospikes regulated myosin IIA recruitment at the cell membrane, enhancing cellular uptake efficiency. Moreover, the spiky nanoparticles facilitated endocytosis dynamics. These findings provide insights into the intracellular fate of nanoparticles and the development of theranostic nanocarriers.
Article
Chemistry, Multidisciplinary
Daoxia Guo, Xiaoyuan Ji, Hui Xie, Jia Ma, Chunchen Xu, Yanfeng Zhou, Nan Chen, Hui Wang, Chunhai Fan, Haiyun Song
Summary: Cancer-associated fibroblasts (CAFs) play a role in chemoresistance and immunosuppression, but direct depletion may lead to increased invasiveness and metastasis. Gemini-like targeting nanoparticles (NPs) have been designed to transform CAFs and eliminate cancer cells, enhancing drug susceptibility and immune cell infiltration. In mouse models of various resistant cancers, a single dose of these NPs resulted in improved chemosensitivity, immune activation, tumor regression, and long-term protection against tumor rechallenge.
ADVANCED MATERIALS
(2023)
Article
Thermodynamics
Guangqing Du, Fangrui Yu, Yu Lu, Lin Kai, Qing Yang, Xun Hou, Feng Chen
Summary: In this study, the ultrafast thermalization dynamics of temperature fields in Au/Ni film excited by a femtosecond laser double-pulse vortex beam were theoretically investigated. It was proposed that the energy deposition in the layered film can be significantly enhanced by applying the double-pulse vortex beam, leading to vortical thermalization in the Ni layer on a picosecond timescale. The intensified dynamics of the double-pulse vortex beam interacting with the excited state of the film and the energy competitive processes of electron diffusion and electron-phonon coupling were identified as the main causes for this phenomenon. The research also explored the relationship between the electron-phonon coupling period of the film and important laser parameters such as pulse separation and vortex beam fluence.
INTERNATIONAL JOURNAL OF THERMAL SCIENCES
(2023)
Article
Physics, Applied
Rui Wu, Feng Chen
Summary: Visualization experiments were conducted on microfluidic pore networks to understand the interactions between salt precipitation, corner liquid film flow, and gas-liquid displacement during evaporation. The study revealed two forms of salt precipitation - aggregated polycrystalline structures and large bulk crystals. It was found that gas bubbles could form due to liquid imbibition into aggregated polycrystalline structures. The length of a corner liquid film affected the growth direction of the aggregated polycrystalline structures.
JOURNAL OF APPLIED PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Xiangyuan Ouyang, Yongli Wu, Yanjing Gao, Lingyun Li, Le Li, Ting Liu, Xinxin Jing, Yue Fu, Jing Luo, Gang Xie, Sisi Jia, Mingqiang Li, Qian Li, Chunhai Fan, Xiaoguo Liu
Summary: Researchers have developed a simple and fast method to synthesize micron-scale amorphous copper nanosheets with a thickness of 1.9 +/- 0.4 nm in aqueous solution at room temperature. These amorphous nanosheets exhibit stronger photoemission and photostability, making them promising for various applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
News Item
Chemistry, Multidisciplinary
Ping Song, Chunhai Fan
Summary: A new high-dimensional microfluidic approach allows for the quantitative isolation of aptamers with customizable binding affinities, presenting a challenging yet promising method for constructing aptamers with desired target-binding affinities.
Article
Chemistry, Multidisciplinary
Haoran Zheng, Haidong Li, Mingqiang Li, Tingting Zhai, Xiaodong Xie, Cong Li, Xinxin Jing, Chengpin Liang, Qian Li, Xiaolei Zuo, Jiang Li, Jiangli Fan, Jianlei Shen, Xiaojun Peng, Chunhai Fan
Summary: Researchers have developed a mechanosensitive DNA nanomachine that can convert membrane tension changes into fluorescence signals. By measuring the fluorescence of the nanomachine, they established a correlation between membrane tension and curvature, which is important for studying membrane mechanics in different contexts.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Physics, Applied
Jialiang Zhang, Qing Yang, Qingyun Ma, Fangzheng Ren, Haoyu Li, Chengjun Zhang, Yang Cheng, Feng Chen
Summary: The study prepared a slippery surface with excellent self-cleaning, stability, and self-healing properties, inspired by the Nepenthes plant. The stretchability of the slippery surface is essential for application in flexible sensor surfaces. By using femtosecond laser, an interconnected porous structure was prepared on pre-stretched polydimethylsiloxane, and then infused with lubricant to create the slippery surface. This stretchable slippery surface maintained its performance even under tensile conditions and after multiple stretch cycles, demonstrating remarkable self-cleaning and chemical stability. The femtosecond laser direct writing technique holds promise for stable surfaces in various extreme environmental applications of flexible electronic devices.
APPLIED PHYSICS LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Kun Dai, Chen Gong, Yang Xu, Fei Ding, Xiaodong Qi, Xinyi Tu, Lu Yu, Xiaoguo Liu, Jiang Li, Chunhai Fan, Hao Yan, Guangbao Yao
Summary: This study presents a transcription-based approach to quantitatively integrate nucleoside analogue molecules into RNA nanostructures. The integrated RNA origami not only maintains its morphology and biostability, but also gains new biomedical functions, such as innate immune recognition after integrating epigenetic nucleoside analogues, and synergistic effects on tumor cell killing after integrating therapeutic nucleoside analogues. This research provides a promising method for the quantitative integration of functional nucleoside analogues into RNA nanostructures, offering valuable insights for the development of multifunctional RNA origamis.
