期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 59, 期 22, 页码 8512-8516出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202002391
关键词
bacterial imaging; enzymes; lanthanides; luminescence; nitroreductase
资金
- Sino-German research project [GZ 1271]
Herein we report the development of a turn-on lanthanide luminescent probe for time-gated detection of nitroreductases (NTRs) in live bacteria. The probe is activated through NTR-induced formation of the sensitizing carbostyril antenna and resulting energy transfer to the lanthanide center. This novel NTR-responsive trigger is virtually non-fluorescent in its inactivated form and features a large signal increase upon activation. We show that the probe is capable of selectively sensing NTR in lysates as well as in live bacteria of the ESKAPE family which are clinically highly relevant multiresistant pathogens responsible for the majority of hospital infections. The results suggest that our probe could be used to develop diagnostic tools for bacterial infections.
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