Fully Exploiting the Power of 2D NMR J-Resolved Spectroscopy

Nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical tool that enables one to study molecular properties and interactions. Homonuclear couplings provide valuable structural information but are often difficult to disentangle in crowded NMR spectra where complex multiplets and signal overlap commonly exist. Multidimensional NMR experiments push 5 the power of H-1 NMR to a new level by providing better signal dispersion. Among them, 2D J-resolved spectroscopy is widely used for multiplet analysis and the measurement of scalar coupling constants. Here, we present a new 2D J-resolved method, CASCADE, through which easier multiplet analysis and unambiguous measurement of specific coupling constants can be achieved at the same time, fully exploiting the power of 2D J-resolved spectroscopy. It is expected that this method may replace a conventional 2D J experiment in many cases, facilitating structural and configurational studies as well as chemical and biological analyses.