The O-antigen of Plesiomonas shigelloides serotype O36 containing pseudaminic acid

The structure of the repeating unit of O-antigen of Plesiomonas shigelloides serotype O36 has been investigated by H-1 and C-13 NMR spectroscopy, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry and chemical methods. The new structure of trisaccharide has been established: -> 4)-beta-Pse5Ac7(R3Hb)-(2 -> 4)-beta-D-Galp-(1 -> 3)-beta-D-GlcpNAc-(1 -> These trisaccharide O-antigen units substitute the core undecasaccharide at C-4 of the beta-D-GlcpNAc residue. The core oligosaccharide and lipid A are identical with these of the serotype 017 (PCM 2231) (Maciejewska, A., Lukasiewicz, J., Kaszowska, M., Jachymek, W., Man-Kupisinska, A.; Lugowski, C. Mar. Drugs. 2013, 11 (2), 440-454; Lukasiewicz, J., Dzieciatkowska, M., Niedziela, T., Jachymek, W., Augustyniuk, A., Kenne, L, Lugowski, C. Biochemistry, 2006, 45, 10434-10447). (C) 2016 Elsevier Ltd. All rights reserved.