Article
Biochemistry & Molecular Biology
Cheng-Chih Lin, Hung-Yin Lin, James L. Thomas, Jia-Xin Yu, Chien-Yu Lin, Yu-Hua Chang, Mei-Hwa Lee, Tzong-Liu Wang
Summary: High-temperature pyrolysis was used to prepare lanthanide-doped UCNPs with LiYF4 for enhanced green luminescence. These UCNPs were entrapped in peptide-imprinted EVAL particles for targeted and photodynamic therapy of PD-L1 in tumor cells. The in vitro cytotoxicity of the composite nanoparticles in HepG2 cells under NIR laser irradiation was evaluated, and both intrinsic and extrinsic pathways of UCNP-embedded molecularly imprinted polymers delivery were investigated.
Article
Biology
Alexander Marquardt, Mario Richterstetter, Helge Taubert, Arndt Hartmann, Bernd Wullich, Verena Lieb, Laura Bellut, Sven Wach, Hendrik Apel
Summary: The use of photodynamic diagnostics (PDD) in bladder cancer treatment leads to improved tumor detection rates and reduced recurrence rates. It also reduces the incidence of residual tumors at the initial resection site in extended transurethral resection of the bladder tumor (TURBT) procedures.
Article
Engineering, Biomedical
Chunhui Wu, Yingxue Zhang, Xiaodan Wei, Ningxi Li, Honglin Huang, Zhengxin Xie, Hanxi Zhang, Geng Yang, Mengyue Li, Tingting Li, Hong Yang, Shun Li, Xiang Qin, Yiyao Liu
Summary: This study presents a novel 2D phototheranostics GMtC against hypoxic solid tumors, integrating tumor homing-penetrating and in situ oxygen-generating functions, showing outstanding performance in tumor deep penetration to hypoxia center and generating abundant oxygen in response to tumor microenvironment, thus exerting the highest efficiency of PDT against 4T1 mammary tumor.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Ying Hao, Yuwen Chen, Xinlong He, Ruxia Han, Chengli Yang, Tailuo Liu, Yun Yang, Qingya Liu, Zhiyong Qian
Summary: To enhance the therapeutic effect of chemo-photodynamic therapy for bladder cancer, researchers constructed a tumor-targeting peptide modified platinum nanozyme, which co-loaded glutathione-responsive prodrug nanoparticles. This system achieved GSH-responsive drug release in tumor microenvironment, enhanced drug accumulation and permeability at tumor sites, and alleviated tumor tissue hypoxia, providing new strategies and methods for clinical treatment of bladder cancer.
Article
Chemistry, Multidisciplinary
Jinrong Ma, Bin Cai, Shuai Zhang, Tengyue Jian, James J. De Yoreo, Chun-Long Chen, Francois Baneyx
Summary: This study reports a simple and modular approach to hierarchical hybrid synthesis by synthesizing peptoid nanosheets and solid-binding protein to construct stacked multilayered materials, which is expected to be applied in bioimaging and photocatalysis fields.
Article
Optics
Sandeep Surendra Panikar, Gonzalo Ramirez-Garcia, Nehla Banu, Alba A. Vallejo-Cardona, Pavel Lugo-Fabres, Tanya A. Camacho-Villegas, Pedro Salas, Elder de la Rosa
Summary: The study demonstrates the effectiveness of methylene blue-based photodynamic therapy using a nano LP system containing MB@UCNPs in killing cancer cells, with enhanced ROS generation capability compared to free MB with UCNPs. The specificity of the treatment was achieved by introducing anti-HER2 peptides for targeting HER-2 overexpressing cells.
JOURNAL OF LUMINESCENCE
(2021)
Article
Pharmacology & Pharmacy
Lin Hao, Zhenduo Shi, Yang Dong, Jiangang Chen, Kun Pang, Houguang He, Shaoqi Zhang, Wei Wu, Qianjin Zhang, Conghui Han
Summary: This study evaluated the efficiency of inhibiting the P3H4 oncogene using tumor-targeting nanoparticles for bladder cancer (BC) therapy. The results showed that CH3-R9-RGD@ce6/siP3H4 nanocomposites could suppress tumor growth and induce apoptosis. These findings suggest that CH3-R9-RGD nanocomposites may be a potential therapeutic strategy for BC.
Article
Chemistry, Analytical
Beatriz A. Braz, Manuel Hospinal-Santiani, Gustavo Martins, Breno C. B. Beirao, Marcio F. Bergamini, Luiz H. Marcolino-Junior, Carlos R. Soccol, Vanete Thomaz-Soccol
Summary: Neglected tropical diseases, which are endemic in low-income populations, lack research investment and have unacceptable indicators. Electrochemical immunosensors show promise for diagnosis in isolated regions. In this study, different peptides were investigated for detecting Leishmania antibodies with outstanding performance achieved using a graphene affinity peptide as a recognition site.
Article
Electrochemistry
Stacy Simai Reginald, Min Ji Kim, Hyeryeong Lee, Nabilah Fazil, Serah Choi, Soyoung Oh, Junhyeok Seo, In Seop Chang
Summary: This study introduces a gold-binding peptide to NAD(+)/NADH-dependent formate dehydrogenase (FDH) through genetic modification, enabling direct wiring of the enzyme to the electrode surface. The gold-binding activity of the fused enzymes depends on fusion site and tertiary structure, with the highest activity observed at the C-terminus. The presence of the gold-binding peptide increases the stability of the enzyme electrode and promotes direct electrochemical reactions. This method shows promise for bio-electronic applications involving NAD(+)/NADH-dependent enzymes.
ELECTROCHIMICA ACTA
(2022)
Article
Oncology
Jiaxin Qin, Qing Liang, Guangyue Wang, Lin Hao, Xing Liu, Xinlei Wang, Zhengxiang Hu, Gaochuan Fang, Liang Xue, Yan Zhao, Rui Li, Qian Lv, Jiling Wen, Guosheng Yang, Conghui Han, Zhenduo Shi
Summary: The synthesized AO-(cRGDfK)(2) probe exhibited nuclear-specific accumulation in bladder cancer cells without affecting cell viability. The probe efficiently targeted tumor cells and did not induce histopathological changes in the heart, liver, spleen, and kidney in vivo.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ayhan Yurtsever, Linhao Sun, Kaito Hirata, Takeshi Fukuma, Siddharth Rath, Hadi Zareie, Shinji Watanabe, Mehmet Sarikaya
Summary: Understanding the mechanisms of self-organization of short peptides is important for the formation of crystalline biomolecular systems and their practical applications. This study focused on the self-organization process of a graphite-binding dodecapeptide on a graphite surface. The observations revealed the formation of homogeneous and heterogeneous layers, with transient crystalline phases, and provided insights into the surface behavior of short peptides on solids.
Article
Chemistry, Analytical
Israel D. Souza, Jared L. Anderson, Maria Eugenia C. Queiroz
Summary: A method for estimating the binding of HSA to A beta in CSF and plasma samples was developed, which may help understand the dynamics of these molecules in the biological system of patients with AD.
ANALYTICA CHIMICA ACTA
(2022)
Article
Multidisciplinary Sciences
Joseph E. Chambers, Nikita Zubkov, Marketa Kubankova, Jonathon Nixon-Abell, Ioanna Mela, Susana Abreu, Max Schwiening, Giulia Lavarda, Ismael Lopez-Duarte, Jennifer A. Dickens, Tomas Torres, Clemens F. Kaminski, Liam J. Holt, Edward Avezov, James A. Huntington, Peter St George-Hyslop, Marina K. Kuimova, Stefan J. Marciniak
Summary: This study investigates the mechanism of protein misfolding in alpha(1)-antitrypsin deficiency, leading to cirrhosis. The researchers find that alpha(1)-antitrypsin polymers undergo a phase transition, forming a protein matrix that hinders the mobility of ER proteins. This phase transition is promoted by ER stress and facilitated by the ER chaperone calreticulin. The study also reveals that immobilization of ER chaperones within the polymer matrix contributes to ER dysfunction. These findings provide insights into proteinopathies and suggest ER chaperones as potential therapeutic targets.
Article
Materials Science, Biomaterials
Shupeng Wang, Shaohua Jin, Guangzhi Li, Ming Xu, Dashi Deng, Zhisheng Xiao, Haiyan Sun, Shaohua Zhang, Enpu Zhang, Lejing Xie, Guo Li, Yizhi Dai, Zhuang Liu, Qinghai Shu, Song Wu
Summary: The combination of nitazoxanide and photosensitizer to form nanoparticles, along with fluorinated chitosan as a transmucosal carrier, can improve the delivery efficiency of intravesical drugs. Meanwhile, the metabolic regulation of tumor cells by nitazoxanide can help ameliorate tumor hypoxia, enhancing the effectiveness of photodynamic therapy.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Review
Biology
Melissa Siaw Han Lim, Takashi Ohtsuki, Fumiaki Takenaka, Kazuko Kobayashi, Masaru Akehi, Hirotaka Uji, Hirotsugu Kobuchi, Takanori Sasaki, Eiichi Ozeki, Eiji Matsuura
Summary: Theranostics, a novel concept in medical advances combining therapeutic and diagnostic systems, shows promising prospects in personalized medicine, particularly in cancer treatment. Utilizing various methods such as targeted apoptosis and photodynamic therapy, this system enables simultaneous treatment and diagnosis.
Article
Biochemistry & Molecular Biology
Hou Liu, Yubin Feng, Songtian Che, Lin Guan, Xinting Yang, Yue Zhao, Linan Fang, Andrei V. Zvyagin, Quan Lin
Summary: This study explored a method of promoting spinal cord injury repair by injecting neural stem cells into the injury site using a biodegradable conductive hydrogel scaffold. Results showed that the conductive hydrogel facilitated the differentiation of stem cells into neurons and inhibited the formation of glial cells and scars. The study provides a new approach for treating spinal cord injuries.
Review
Materials Science, Biomaterials
Ze Wang, Xiaojun Ren, Dongzhou Wang, Lin Guan, Xingchen Li, Yue Zhao, Annan Liu, Liang He, Tiejun Wang, Andrei V. Zvyagin, Bai Yang, Quan Lin
Summary: Radiotherapy is a commonly used cancer treatment, but the existing radiosensitizers have limitations. Emerging gold-based nanomaterials have shown unique advantages in enhancing radiosensitization, providing a new idea for improving radiotherapy efficacy and reducing side effects. This review systematically summarizes the recent progress of gold-based nanomaterials in mediating radiosensitization, and discusses the challenges and prospects in the field.
BIOMATERIALS SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Vera L. Kovalenko, Elena N. Komedchikova, Anna S. Sogomonyan, Ekaterina D. Tereshina, Olga A. Kolesnikova, Aziz B. Mirkasymov, Anna M. Iureva, Andrei V. Zvyagin, Petr I. Nikitin, Victoria O. Shipunova
Summary: This article presents a new strategy for the treatment of aggressive tumors using lectin-equipped polymer nanoparticles for targeted delivery and photodynamic therapy. The experiments showed that this treatment method achieved high tumor growth inhibition even for large solid tumors.
Review
Biology
Olga M. Kutova, Anton D. Pospelov, Irina V. Balalaeva
Summary: Connexins are proteins that make up gap junctions in cells, which connect neighboring cells and the cell interior with the extracellular microenvironment. They play a role in tissue integration. Changes in connexin regulation can lead to unfavorable shifts in the tissue environment and enhance cell motility. This review examines the role of connexins in the tumor microenvironment and their involvement in cancer.
Article
Engineering, Biomedical
Ze Wang, Huiyuan Xing, Annan Liu, Lin Guan, Xingchen Li, Liang He, Yuanqing Sun, Andrei V. Zvyagin, Bai Yang, Quan Lin
Summary: A targeted theranostic platform of AMNDs-LHRH nano-system has been reported for multi-mode imaging guided photothermal therapy of metastatic prostate cancer. The nano-system can simultaneously target prostate cancer and its metastases for accurate preoperative CT/MR diagnosis, and it also possesses fluorescence visualization navigated surgery, demonstrating its potential application in clinical cancer detection and surgery guidance.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Analytical
Ze Wang, Annan Liu, Xingchen Li, Lin Guan, Huiyuan Xing, Liang He, Linan Fang, Andrei V. Zvyagin, Xiaoyu Yang, Bai Yang, Quan Lin
Summary: The high incidence and complex subtypes of prostate cancer require accurate diagnosis. A multifunctional nanoprobe Au/Gd nanodots were designed and fabricated for multi-mode imaging and accurate diagnosis of bone metastatic prostate cancer. The results showed that combining multiple imaging modes achieved high-precision detection and accurate treatment guidance. Additionally, the Au/Gd nanodots demonstrated good biocompatibility and biosafety in in vitro/vivo experiments, making them a potential strategy for precise diagnosis in metastatic prostate cancer.
Review
Chemistry, Multidisciplinary
Laura M. Rey Gomez, Rena Hirani, Andrew Care, David W. Inglis, Yuling Wang
Summary: Blood testing is crucial for diagnosis and monitoring of various conditions, and microfluidic devices offer a simpler and more affordable solution for blood sample preparation. However, currently there are limited microfluidic devices designed for undiluted whole blood, which can eliminate the need for blood dilution and minimize sample preparation.
Article
Chemistry, Multidisciplinary
Ze Wang, Dongzhou Wang, Xiaojun Ren, Zhongshan Liu, Annan Liu, Xingchen Li, Lin Guan, Yannan Shen, Shunzi Jin, Andrei V. Zvyagin, Bai Yang, Tiejun Wang, Quan Lin
Summary: In this study, a multifunctional theranostic platform using Au/Ag nanodots was developed to provide real-time guidance for surgery, high-efficiency radiotherapy, and brachytherapy. The platform offered dual-mode imaging for precise treatment and improved the accuracy of tumor resection during surgery.
ACS CENTRAL SCIENCE
(2023)
Article
Cell Biology
Anton Pospelov, Olga Kutova, Yuri Efremov, Albina Nekrasova, Daria Trushina, Sofia Gefter, Elena Cherkasova, Lidia Timofeeva, Peter Timashev, Andrei Zvyagin, Irina Balalaeva
Summary: Tissue engineering using decellularized tissues (DCL) is a valuable tool for reconstructing organ-specific environments. A universal decellularization protocol for murine organs was developed, demonstrating robustness, reproducibility, and preservation of architecture. The biomechanical properties of the DCL organs were crucial in determining the fate and progression of cancer cells.
Article
Pharmacology & Pharmacy
Tikhon S. Redkin, Ekaterina E. Sleptsova, Victoria D. Turubanova, Mariia O. Saviuk, Svetlana A. Lermontova, Larisa G. Klapshina, Nina N. Peskova, Irina V. Balalaeva, Olga Krysko, Tatiana A. Mishchenko, Maria V. Vedunova, Dmitri V. Krysko
Summary: Research in the past decade has shown that the immunogenicity of dying tumor cells is crucial for effective anticancer therapy. In this study, we characterized the abilities of two photoactive dyes to induce immunogenic cell death (ICD) in glioma cells. We demonstrated that these dyes can efficiently induce ICD and stimulate anti-tumor immune responses, leading to the elimination of tumor cells and the formation of long-term immunological memory.
Review
Biophysics
N. Yu. Shilyagina, L. N. Shestakova, N. N. Peskova, S. A. Lermontova, T. S. Lyubova, L. G. Klapshina, I. V. Balalaeva
Summary: This article reviews cyanoarylporphyrazine dyes synthesized for photodynamic (anticancer) therapy, providing information on their chemistry, photophysical properties, cellular uptake features, and delivery to tumors using various carriers. The potential of cyanoarylporphyrazines as photodynamic anti-tumor agents is evaluated, highlighting their unique feature as viscosity sensors. The authors expect that the combination of photosensitizer and viscosity sensor properties of cyanoarylporphyrazines will provide a tool for dosimetry and personalized treatment regimens in photodynamic therapy.
BIOPHYSICAL REVIEWS
(2023)
Article
Pharmacology & Pharmacy
Claire Rennie, Caitlin Sives, India Boyton, Dennis Diaz, Catherine Gorrie, Orazio Vittorio, Lyndsey Collins-Praino, Andrew Care
Summary: Encapsulins, self-assembling protein nanocages derived from prokaryotes, show promise as nanoparticle-based drug delivery systems. However, their in vivo behavior and fate remain poorly understood. This study investigates the interactions between encapsulins and key biological barriers encountered in drug delivery, revealing their safety, immunogenicity, protein corona, biodistribution, and clearance profiles. These findings provide critical insights into the behavior of encapsulins in vivo, informing their design and application in targeted drug delivery.
ADVANCED THERAPEUTICS
(2023)
Article
Microbiology
Angela Sun, Paul M. Mirzayans, Andrew M. Piggott, Jo-Ann L. Stanton, Anwar Sunna
Summary: This work builds on a previously developed workflow for rapid and sensitive pathogen detection. It establishes a sample treatment strategy that consistently produces quantification efficiencies (QEs) for Campylobacter jejuni against a complex and highly variable sample matrix from a suburban river. The most effective treatments at minimizing the inhibitory effects of the sample matrix were pH buffering with HEPES and addition of the surfactant Tween 20. The workflow's consistency and scalability make it a suitable alternative to culture-based ISO methods for detecting Campylobacter spp. in environmental water.
FEMS MICROBIOLOGY ECOLOGY
(2023)
Article
Immunology
India Boyton, Stella M. Valenzuela, Lyndsey E. Collins-Praino, Andrew Care
Summary: Neuronanomedicine is a multidisciplinary field that aims to develop innovative nanotechnologies for the treatment of neurodegenerative disorders. Nanoparticles are an important component of neuronanomedicine, as they can improve drug properties and enhance safety and delivery across the blood-brain barrier. However, a major challenge for neuronanomedicine translation is the poor understanding of bio-nano interactions, partly due to inconsistent reporting in published works. This review provides specific recommendations to guide researchers in harnessing the unique properties of nanoparticles for breakthrough treatments of Alzheimer's and Parkinson's disease.
BRAIN BEHAVIOR AND IMMUNITY
(2024)
