Exploiting B Cell Receptor Analyses to Inform on HIV-1 Vaccination Strategies

OLGA: fast computation of generation probabilities of B- and T-cell receptor amino acid sequences and motifs

(2019) Zachary Sethna et al.   BIOINFORMATICS

Commonality despite exceptional diversity in the baseline human antibody repertoire

(2019) Bryan Briney et al.   NATURE

Shaping of infant B cell receptor repertoires by environmental factors and infectious disease

(2019) Sandra C. A. Nielsen et al.   Science Translational Medicine

High frequency of shared clonotypes in human B cell receptor repertoires

(2019) Cinque Soto et al.   NATURE

Therapeutic Monoclonal Antibodies for Ebola Virus Infection Derived from Vaccinated Humans

(2019) Pramila Rijal et al.   Cell Reports

Rapid and Focused Maturation of a VRC01-Class HIV Broadly Neutralizing Antibody Lineage Involves Both Binding and Accommodation of the N276-Glycan

(2019) Jeffrey Umotoy et al.   IMMUNITY

Repertoire and Neutralizing Activity of Antibodies Against Hepatitis C Virus E2 Peptide in Patients With Spontaneous Resolution of Hepatitis C

(2019) Anne Olbrich et al.   JOURNAL OF INFECTIOUS DISEASES

High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes

(2019) Mandeep Singh et al.   Nature Communications

Polyclonal and convergent antibody response to Ebola virus vaccine rVSV-ZEBOV

(2019) Stefanie A. Ehrhardt et al.   NATURE MEDICINE

A generalized HIV vaccine design strategy for priming of broadly neutralizing antibody responses

(2019) Jon M. Steichen et al.   SCIENCE

High-Throughput Mapping of B Cell Receptor Sequences to Antigen Specificity

(2019) Ian Setliff et al.   CELL

Identification of Near-Pan-neutralizing Antibodies against HIV-1 by Deconvolution of Plasma Humoral Responses

(2018) Mohammad Mohseni Sajadi et al.   CELL

Multi-Donor Longitudinal Antibody Repertoire Sequencing Reveals the Existence of Public Antibody Clonotypes in HIV-1 Infection

(2018) Ian Setliff et al.   Cell Host & Microbe

HIV-1 Vaccines Based on Antibody Identification, B Cell Ontogeny, and Epitope Structure

(2018) Peter D. Kwong et al.   IMMUNITY

iReceptor: A platform for querying and analyzing antibody/B-cell and T-cell receptor repertoire data across federated repositories

(2018) Brian D. Corrie et al.   IMMUNOLOGICAL REVIEWS

Cross-specificity of protective human antibodies against Klebsiella pneumoniae LPS O-antigen

(2018) Tim Rollenske et al.   NATURE IMMUNOLOGY

The human naive B cell repertoire contains distinct subclasses for a germline-targeting HIV-1 vaccine immunogen

(2018) Colin Havenar-Daughton et al.   Science Translational Medicine

Structural characterization of a highly-potent V3-glycan broadly neutralizing antibody bound to natively-glycosylated HIV-1 envelope

(2018) Christopher O. Barnes et al.   Nature Communications

High-throughput immune repertoire analysis with IGoR

(2018) Quentin Marcou et al.   Nature Communications

Single-Cell Droplet Microfluidic Screening for Antibodies Specifically Binding to Target Cells

(2018) Nachiket Shembekar et al.   Cell Reports

5′ Rapid Amplification of cDNA Ends and Illumina MiSeq Reveals B Cell Receptor Features in Healthy Adults, Adults With Chronic HIV-1 Infection, Cord Blood, and Humanized Mice

(2018) Eric Waltari et al.   Frontiers in Immunology

Analyzing Immunoglobulin Repertoires

(2018) Neha Chaudhary et al.   Frontiers in Immunology

Computational Strategies for Dissecting the High-Dimensional Complexity of Adaptive Immune Repertoires

(2018) Enkelejda Miho et al.   Frontiers in Immunology

When designing vaccines, consider the starting material: the human B cell repertoire

(2018) Colin Havenar-Daughton et al.   CURRENT OPINION IN IMMUNOLOGY

Combination therapy with anti-HIV-1 antibodies maintains viral suppression

(2018) Pilar Mendoza et al.   NATURE

Safety and antiviral activity of combination HIV-1 broadly neutralizing antibodies in viremic individuals

(2018) Yotam Bar-On et al.   NATURE MEDICINE

Beyond Hot Spots: Biases in Antibody Somatic Hypermutation and Implications for Vaccine Design

(2018) Chaim A. Schramm et al.   Frontiers in Immunology

AIRR Community Standardized Representations for Annotated Immune Repertoires

(2018) Jason Anthony Vander Heiden et al.   Frontiers in Immunology

Recent progress in broadly neutralizing antibodies to HIV

(2018) Devin Sok et al.   NATURE IMMUNOLOGY

A Broadly Neutralizing Antibody Targets the Dynamic HIV Envelope Trimer Apex via a Long, Rigidified, and Anionic β-Hairpin Structure

(2017) Jeong Hyun Lee et al.   IMMUNITY

HIV Envelope Glycoform Heterogeneity and Localized Diversity Govern the Initiation and Maturation of a V2 Apex Broadly Neutralizing Antibody Lineage

(2017) Elise Landais et al.   IMMUNITY

Virus-like Particles Identify an HIV V1V2 Apex-Binding Neutralizing Antibody that Lacks a Protruding Loop

(2017) Evan M. Cale et al.   IMMUNITY

Active droplet sorting in microfluidics: a review

(2017) Heng-Dong Xi et al.   LAB ON A CHIP

Single-cell deep phenotyping of IgG-secreting cells for high-resolution immune monitoring

(2017) Klaus Eyer et al.   NATURE BIOTECHNOLOGY

Antibody 10-1074 suppresses viremia in HIV-1-infected individuals

(2017) Marina Caskey et al.   NATURE MEDICINE

Broadly neutralizing antibodies targeting the HIV-1 envelope V2 apex confer protection against a clade C SHIV challenge

(2017) Boris Julg et al.   Science Translational Medicine

Coexistence of potent HIV-1 broadly neutralizing antibodies and antibody-sensitive viruses in a viremic controller

(2017) Natalia T. Freund et al.   Science Translational Medicine

Staged induction of HIV-1 glycan–dependent broadly neutralizing antibodies

(2017) Mattia Bonsignori et al.   Science Translational Medicine

Neutralization of Zika virus by germline-like human monoclonal antibodies targeting cryptic epitopes on envelope domain III

(2017) Yanling Wu et al.   Emerging Microbes & Infections

HIV-1 Neutralizing Antibodies with Limited Hypermutation from an Infant

(2016) Cassandra A. Simonich et al.   CELL

Vaccine-Induced Antibodies that Neutralize Group 1 and Group 2 Influenza A Viruses

(2016) M. Gordon Joyce et al.   CELL

Maturation Pathway from Germline to Broad HIV-1 Neutralizer of a CD4-Mimic Antibody

(2016) Mattia Bonsignori et al.   CELL

Development of broad-spectrum human monoclonal antibodies for rabies post-exposure prophylaxis

(2016) P. De Benedictis et al.   EMBO Molecular Medicine

Early Antibody Lineage Diversification and Independent Limb Maturation Lead to Broad HIV-1 Neutralization Targeting the Env High-Mannose Patch

(2016) Daniel T. MacLeod et al.   IMMUNITY

Identification of a CD4-Binding-Site Antibody to HIV that Evolved Near-Pan Neutralization Breadth

(2016) Jinghe Huang et al.   IMMUNITY

Structures of Preferred Human IgV Genes–Based Protective Antibodies Identify How Conserved Residues Contact Diverse Antigens and Assign Source of Specificity to CDR3 Loop Variation

(2016) Steve Bryson et al.   JOURNAL OF IMMUNOLOGY

HIV-1 antibody 3BNC117 suppresses viral rebound in humans during treatment interruption

(2016) Johannes F. Scheid et al.   NATURE

A single injection of anti-HIV-1 antibodies protects against repeated SHIV challenges

(2016) Rajeev Gautam et al.   NATURE

Neutralizing human antibodies prevent Zika virus replication and fetal disease in mice

(2016) Gopal Sapparapu et al.   NATURE

Ultra-high-throughput sequencing of the immune receptor repertoire from millions of lymphocytes

(2016) Jonathan R McDaniel et al.   Nature Protocols

Effect of HIV Antibody VRC01 on Viral Rebound after Treatment Interruption

(2016) Katharine J. Bar et al.   NEW ENGLAND JOURNAL OF MEDICINE

Hierarchy and extremes in selections from pools of randomized proteins

(2016) Sébastien Boyer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Long antibody HCDR3s from HIV-naïve donors presented on a PG9 neutralizing antibody background mediate HIV neutralization

(2016) Jordan R. Willis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Large-scale sequence and structural comparisons of human naive and antigen-experienced antibody repertoires

(2016) Brandon J. DeKosky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

HIV-1 broadly neutralizing antibody precursor B cells revealed by germline-targeting immunogen

(2016) J. G. Jardine et al.   SCIENCE

Fusion peptide of HIV-1 as a site of vulnerability to neutralizing antibody

(2016) R. Kong et al.   SCIENCE

Isolation of potent neutralizing antibodies from a survivor of the 2014 Ebola virus outbreak

(2016) Z. A. Bornholdt et al.   SCIENCE

Specificity, cross-reactivity, and function of antibodies elicited by Zika virus infection

(2016) K. Stettler et al.   SCIENCE

Differences in Allelic Frequency and CDRH3 Region Limit the Engagement of HIV Env Immunogens by Putative VRC01 Neutralizing Antibody Precursors

(2016) Christina Yacoob et al.   Cell Reports

B-cell repertoire dynamics after sequential hepatitis B vaccination and evidence for cross-reactive B-cell activation

(2016) Jacob D. Galson et al.   Genome Medicine

Lower IgG somatic hypermutation rates during acute dengue virus infection is compatible with a germinal center-independent B cell response

(2016) Elizabeth Ernestina Godoy-Lozano et al.   Genome Medicine

An HIV-1 antibody from an elite neutralizer implicates the fusion peptide as a site of vulnerability

(2016) Marit J. van Gils et al.   Nature Microbiology

Developmental Pathway of the MPER-Directed HIV-1-Neutralizing Antibody 10E8

(2016) Cinque Soto et al.   PLoS One

Structural Repertoire of HIV-1-Neutralizing Antibodies Targeting the CD4 Supersite in 14 Donors

(2015) Tongqing Zhou et al.   CELL

Maturation and Diversity of the VRC01-Antibody Lineage over 15 Years of Chronic HIV-1 Infection

(2015) Xueling Wu et al.   CELL

Fluorescently labeled dengue viruses as probes to identify antigen-specific memory B cells by multiparametric flow cytometry

(2015) Marcia Woda et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Ability To Develop Broadly Neutralizing HIV-1 Antibodies Is Not Restricted by the Germline Ig Gene Repertoire

(2015) Cathrine Scheepers et al.   JOURNAL OF IMMUNOLOGY

New Member of the V1V2-Directed CAP256-VRC26 Lineage That Shows Increased Breadth and Exceptional Potency

(2015) Nicole A. Doria-Rose et al.   JOURNAL OF VIROLOGY

Viraemia suppressed in HIV-1-infected humans by broadly neutralizing antibody 3BNC117

(2015) Marina Caskey et al.   NATURE

Viral variants that initiate and drive maturation of V1V2-directed HIV-1 broadly neutralizing antibodies

(2015) Jinal N Bhiman et al.   NATURE MEDICINE

Dynamics of immunoglobulin sequence diversity in HIV-1 infected individuals

(2015) Kenneth B. Hoehn et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Inferring processes underlying B-cell repertoire diversity

(2015) Yuval Elhanati et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Virologic effects of broadly neutralizing antibody VRC01 administration during chronic HIV-1 infection

(2015) Rebecca M. Lynch et al.   Science Translational Medicine

Bioinformatic and Statistical Analysis of Adaptive Immune Repertoires

(2015) Victor Greiff et al.   TRENDS IN IMMUNOLOGY

Cooperation of B Cell Lineages in Induction of HIV-1-Broadly Neutralizing Antibodies

(2014) Feng Gao et al.   CELL

Human Responses to Influenza Vaccination Show Seroconversion Signatures and Convergent Antibody Rearrangements

(2014) Katherine J.L. Jackson et al.   Cell Host & Microbe

High-throughput sequencing of natively paired antibody chains provides evidence for original antigenic sin shaping the antibody response to influenza vaccination

(2014) Yann-Chong Tan et al.   CLINICAL IMMUNOLOGY

Passive transfer of modest titers of potent and broadly neutralizing anti-HIV monoclonal antibodies block SHIV infection in macaques

(2014) Masashi Shingai et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Immunodominance of the VH1-46 Antibody Gene Segment in the Primary Repertoire of Human Rotavirus-Specific B Cells Is Reduced in the Memory Compartment through Somatic Mutation of Nondominant Clones

(2014) C. Tian et al.   JOURNAL OF IMMUNOLOGY

Enhanced Potency of a Broadly Neutralizing HIV-1 Antibody In Vitro Improves Protection against Lentiviral Infection In Vivo

(2014) R. S. Rudicell et al.   JOURNAL OF VIROLOGY

Rapid development of broadly influenza neutralizing antibodies through redundant mutations

(2014) Leontios Pappas et al.   NATURE

Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies

(2014) Nicole A. Doria-Rose et al.   NATURE

In-depth determination and analysis of the human paired heavy- and light-chain antibody repertoire

(2014) Brandon J DeKosky et al.   NATURE MEDICINE

Recombinant HIV envelope trimer selects for quaternary-dependent antibodies targeting the trimer apex

(2014) Devin Sok et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

(2014) Brian D Plouffe et al.   REPORTS ON PROGRESS IN PHYSICS

Toward a more accurate view of human B-cell repertoire by next-generation sequencing, unbiased repertoire capture and single-molecule barcoding

(2014) Linling He et al.   Scientific Reports

IgG Subclasses and Allotypes: From Structure to Effector Functions

(2014) Gestur Vidarsson et al.   Frontiers in Immunology

The potential of the human immune system to develop broadly neutralizing HIV-1 antibodies

(2013) Yu Zhang et al.   AIDS

Somatic Mutations of the Immunoglobulin Framework Are Generally Required for Broad and Potent HIV-1 Neutralization

(2013) Florian Klein et al.   CELL

Mechanisms of Programmed DNA Lesions and Genomic Instability in the Immune System

(2013) Frederick W. Alt et al.   CELL

Single-cell based high-throughput sequencing of full-length immunoglobulin heavy and light chain genes

(2013) Christian E. Busse et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

Deep sequencing and Circos analyses of antibody libraries reveal antigen-driven selection of Ig VH genes during HIV-1 infection

(2013) Madelyne Xiao et al.   EXPERIMENTAL AND MOLECULAR PATHOLOGY

Multidonor Analysis Reveals Structural Elements, Genetic Determinants, and Maturation Pathway for HIV-1 Neutralization by VRC01-Class Antibodies

(2013) Tongqing Zhou et al.   IMMUNITY

HIV-1 neutralizing antibodies: understanding nature's pathways

(2013) John R. Mascola et al.   IMMUNOLOGICAL REVIEWS

Co-evolution of a broadly neutralizing HIV-1 antibody and founder virus

(2013) Hua-Xin Liao et al.   NATURE

Single-cell analysis and sorting using droplet-based microfluidics

(2013) Linas Mazutis et al.   Nature Protocols

Genetic measurement of memory B-cell recall using antibody repertoire sequencing

(2013) C. Vollmers et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mining the antibodyome for HIV-1-neutralizing antibodies with next-generation sequencing and phylogenetic pairing of heavy/light chains

(2013) J. Zhu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

De novo identification of VRC01 class HIV-1-neutralizing antibodies by next-generation sequencing of B-cell transcripts

(2013) J. Zhu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Delineating Antibody Recognition in Polyclonal Sera from Patterns of HIV-1 Isolate Neutralization

(2013) I. S. Georgiev et al.   SCIENCE

Antibodies in HIV-1 Vaccine Development and Therapy

(2013) F. Klein et al.   SCIENCE

Correction: Recombinant HIV Envelope Proteins Fail to Engage Germline Versions of Anti-CD4bs bNAbs

(2013) Sam Hoot et al.   PLoS Pathogens

Correction: The Effects of Somatic Hypermutation on Neutralization and Binding in the PGT121 Family of Broadly Neutralizing HIV Antibodies

(2013) Devin Sok et al.   PLoS Pathogens

A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies

(2013) Rogier W. Sanders et al.   PLoS Pathogens

Somatic populations of PGT135–137 HIV-1-neutralizing antibodies identified by 454 pyrosequencing and bioinformatic

(2013) Jiang Zhu   Frontiers in Microbiology

IgM Repertoire Biodiversity is Reduced in HIV-1 Infection and Systemic Lupus Erythematosus

(2013) Li Yin et al.   Frontiers in Immunology

Models of Somatic Hypermutation Targeting and Substitution Based on Synonymous Mutations from High-Throughput Immunoglobulin Sequencing Data

(2013) Gur Yaari et al.   Frontiers in Immunology

Germinal Centers

(2012) Gabriel D. Victora et al.   Annual Review of Immunology

Characterization of human IgG repertoires in an acute HIV-1 infection

(2012) Weizao Chen et al.   EXPERIMENTAL AND MOLECULAR PATHOLOGY

Broad neutralization by a combination of antibodies recognizing the CD4 binding site and a new conformational epitope on the HIV-1 envelope protein

(2012) Florian Klein et al.   JOURNAL OF EXPERIMENTAL MEDICINE

B-cell–lineage immunogen design in vaccine development with HIV-1 as a case study

(2012) Barton F Haynes et al.   NATURE BIOTECHNOLOGY

Antibody Repertoires in Humanized NOD-scid-IL2Rγnull Mice and Human B Cells Reveals Human-Like Diversification and Tolerance Checkpoints in the Mouse

(2012) Gregory C. Ippolito et al.   PLoS One

Human Peripheral Blood Antibodies with Long HCDR3s Are Established Primarily at Original Recombination Using a Limited Subset of Germline Genes

(2012) Bryan S. Briney et al.   PLoS One

Highly potent HIV-specific antibody neutralization in vitro translates into effective protection against mucosal SHIV challenge in vivo

(2012) B. Moldt et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural basis for germ-line gene usage of a potent class of antibodies targeting the CD4-binding site of HIV-1 gp120

(2012) Anthony P. West et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9

(2011) Jason S. McLellan et al.   NATURE

Beyond natural antibodies: the power of in vitro display technologies

(2011) Andrew R M Bradbury et al.   NATURE BIOTECHNOLOGY

Naive antibody gene-segment frequencies are heritable and unaltered by chronic lymphocyte ablation

(2011) J. Glanville et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Focused Evolution of HIV-1 Neutralizing Antibodies Revealed by Structures and Deep Sequencing

(2011) X. Wu et al.   SCIENCE

Sequence and Structural Convergence of Broad and Potent HIV Antibodies That Mimic CD4 Binding

(2011) J. F. Scheid et al.   SCIENCE

A Neutralizing Antibody Selected from Plasma Cells That Binds to Group 1 and Group 2 Influenza A Hemagglutinins

(2011) D. Corti et al.   SCIENCE

High-throughput immunoglobulin repertoire analysis distinguishes between human IgM memory and switched memory B-cell populations

(2010) Y.-C. Wu et al.   BLOOD

Pseudovirion Particles Bearing Native HIV Envelope Trimers Facilitate a Novel Method for Generating Human Neutralizing Monoclonal Antibodies Against HIV

(2010) Mark D Hicar et al.   JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES

Structure and function of immunoglobulins

(2010) Harry W. Schroeder et al.   JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

Heterosubtypic neutralizing antibodies are produced by individuals immunized with a seasonal influenza vaccine

(2010) Davide Corti et al.   JOURNAL OF CLINICAL INVESTIGATION

Sequencing and Quantifying IgG Fragments and Antigen-Binding Regions by Mass Spectrometry

(2010) Dominique de Costa et al.   JOURNAL OF PROTEOME RESEARCH

V-gene amplification revisited – An optimised procedure for amplification of rearranged human antibody genes of different isotypes

(2010) Theam Soon Lim et al.   New Biotechnology

B cell gene signature with massive intrahepatic production of antibodies to hepatitis B core antigen in hepatitis B virus-associated acute liver failure

(2010) P. Farci et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural Basis for Broad and Potent Neutralization of HIV-1 by Antibody VRC01

(2010) T. Zhou et al.   SCIENCE

Rational Design of Envelope Identifies Broadly Neutralizing Human Monoclonal Antibodies to HIV-1

(2010) X. Wu et al.   SCIENCE

Efficient Recovery of High-Affinity Antibodies from a Single-Chain Fab Yeast Display Library

(2009) Laura M. Walker et al.   JOURNAL OF MOLECULAR BIOLOGY

Broad diversity of neutralizing antibodies isolated from memory B cells in HIV-infected individuals

(2009) Johannes F. Scheid et al.   NATURE

Structural and functional bases for broad-spectrum neutralization of avian and human influenza A viruses

(2009) Jianhua Sui et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Broad and Potent Neutralizing Antibodies from an African Donor Reveal a New HIV-1 Vaccine Target

(2009) L. M. Walker et al.   SCIENCE

Measurement and Clinical Monitoring of Human Lymphocyte Clonality by Massively Parallel V-D-J Pyrosequencing

(2009) S. D. Boyd et al.   Science Translational Medicine

B lymphocytes: how they develop and function

(2008) T. W. LeBien et al.   BLOOD

Germline V-genes sculpt the binding site of a family of antibodies neutralizing human cytomegalovirus

(2008) Christy A Thomson et al.   EMBO JOURNAL

A method for identification of HIV gp140 binding memory B cells in human blood

(2008) Johannes F. Scheid et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Preferential use of the VH5-51 gene segment by the human immune response to code for antibodies against the V3 domain of HIV-1

(2008) Miroslaw K. Gorny et al.   MOLECULAR IMMUNOLOGY

Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning

(2007) Thomas Tiller et al.   JOURNAL OF IMMUNOLOGICAL METHODS