Article
Engineering, Biomedical
Ze Lin, Yuan Xiong, Weilin Meng, Yiqiang Hu, Lili Chen, Lang Chen, Hang Xue, Adriana C. Panayi, Wu Zhou, Yun Sun, Faqi Cao, Guodong Liu, Liangcong Hu, Chenchen Yan, Xudong Xie, Chuanchuan Lin, Kaiyong Cai, Qian Feng, Bobin Mi, Guohui Liu
Summary: This study demonstrates the use of PD-L1-enriched exosomes for bone fracture repair and highlights the potential of Hydrogel@Exos systems for bone fracture therapy through immune inhibitory effects. The results show that exosomal PD-L1 can inhibit T cell activation and promote osteogenic differentiation of mesenchymal stem cells. Additionally, embedding exosomes in a hydrogel allows for time-released delivery and improves treatment efficacy.
BIOACTIVE MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Ye Wang, Changyuan He, Chong Chen, Wentao Dong, Xuekun Yang, Ye Wu, Qingquan Kong, Bin Yan
Summary: In this study, an injectable zwitterionic hydrogel with excellent self-healing property, good cytocompatibility, and antibacterial adhesion was developed. The hydrogel showed a distinct thermal-induced sol-gel transition and could be easily applied to complex wounds. It possessed rapid self-healing ability and low cytotoxicity, and effectively prevented bacterial adhesion, promoting wound healing.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Oncology
Valentin Vautrot, Hafidha Bentayeb, Sebastien Causse, Carmen Garrido, Jessica Gobbo
Summary: Immunotherapy, particularly through the blockade of immune checkpoints such as PD-1/PD-L1, has become increasingly important in cancer treatment by reactivating the immune system to fight cancer cells. However, resistance to these treatments in a certain proportion of patients has led to efforts to combine PD-1/PD-L1 immunotherapy with targeting other immune checkpoints to improve outcomes. Exosomes, small vesicles secreted by various cells including tumor cells, play a key role in mediating immune resistance and establishing metastatic niches. Research on specific exosomal proteins like PD-L1, CTLA-4, TIM-3, CD73/39, LAG-3, and TIGIT aims to enhance understanding of tumor resistance mechanisms and guide therapeutic decisions in immunotherapy.
Article
Chemistry, Multidisciplinary
Bingqian Lin, Tian Tian, Yinzhu Lu, Dan Liu, Mengjiao Huang, Lin Zhu, Zhi Zhu, Yanling Song, Chaoyong Yang
Summary: The TRACER system utilizes a dual-target-specific aptamer recognition activated in situ connection system combined with ddPCR technology to quantitatively detect tumor-derived exosomal PD-L1 in a wash-free manner, showing significant sensitivity and selectivity. This method has the potential to serve as a more reliable tumor diagnostic marker than total Exo-(PD-L1) and to explore the biological functions of exosomes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Engineering, Environmental
Kai Wang, Ruonan Dong, Jiezhang Tang, Huichen Li, Juanli Dang, Zhaoxiang Zhang, Zhou Yu, Baolin Guo, Chenggang Yi
Summary: This study developed a self-healing hydrogel capable of stably releasing exosomes to enhance diabetic wound healing. Through the synergistic effect of exosome release and M2 macrophage polarization, it accelerated angiogenesis and wound healing.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Biomedical
Junkai Zeng, Zhenyu Sun, Feihui Zeng, Changjiang Gu, Xiongsheng Chen
Summary: Current treatments for diabetic wounds are unsatisfactory due to local overactive inflammatory response and impaired angiogenesis. In this study, a double-layer microneedle-based wound dressing system called MEs@PMN was developed to suppress inflammation and improve angiogenesis. The encapsulated M2 macrophage-derived exosomes (MEs) in the needle tips modulated macrophage polarization towards the M2 phenotype, while the backing layer containing polydopamine (PDA) nanoparticles generated mild heat to improve angiogenesis. The MEs@PMN also showed promising effects in diabetic rats by inhibiting uncontrolled inflammatory response and promoting vascular regeneration.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Environmental
Yiqiang Hu, Bin Wu, Yuan Xiong, Ranyang Tao, Adriana C. Panayi, Lang Chen, Wenqing Tian, Hang Xue, Lei Shi, Xianglin Zhang, Liming Xiong, Bobin Mi, Guohui Liu
Summary: This study utilizes a novel 3D hydrogel scaffold to accelerate the healing of diabetic wounds, including increasing blood flow, stimulating angiogenesis, and promoting the formation of crucial blood vessel tissues.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Weichang Li, Shujie Wu, Lin Ren, Bingyu Feng, Zhipei Chen, Zongtai Li, Bin Cheng, Juan Xia
Summary: A hydrogel system integrating gold nanorods and M2 macrophage-derived exosomes was developed to achieve efficient therapy for diabetic wounds. The system effectively scavenged reactive oxygen species, inhibited inflammation, promoted angiogenesis, and stimulated photothermal antibacterial activity through near-infrared irradiation, demonstrating potential for chronic wound healing and tissue regeneration in diabetic patients.
Review
Cell Biology
Seyed Z. Rasihashemi, Erfan Rezazadeh Gavgani, Reza Majidazar, Parya Seraji, Mobina Oladghaffari, Tohid Kazemi, Parisa Lotfinejad
Summary: The article discusses the potential mechanism of exosomal PD-L1 in immune checkpoint therapy failure and explores the inhibition of exosome biogenesis as a new strategy to overcome tumor resistance to anti-PD-L1 therapy. The diagnostic and prognostic value of exosomal PD-L1 in different cancer types is also discussed.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiao-Hui Wang, Wei Guo, Wei Qiu, Luo-Quan Ao, Meng-Wei Yao, Wei Xing, Yang Yu, Quan Chen, Xiao-Feng Wu, Zhan Li, Xue-Ting Hu, Xiang Xu
Summary: This study reveals a novel function of PD-L1 in wound healing, showing that PD-L1 acts as a positive regulator by forming an immunosuppressive microenvironment, modulating macrophage polarization and promoting inflammation resolution. Loss of PD-L1 delays wound healing, especially in the presence of severe inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Meng Yuan, Kun Liu, Tao Jiang, Shengbo Li, Jing Chen, Zihan Wu, Wenqing Li, Rongzhi Tan, Wenying Wei, Xiaofan Yang, Honglian Dai, Zhenbing Chen
Summary: Clinical work and research on diabetic wound repair are challenging, and conventional wound dressings have limited efficacy. Sustained release of biomolecules from biological wound dressings, such as cell-derived exosomes, shows promise for wound healing. A methacrylate gelatin microneedle patch was developed to achieve transdermal and controlled release of exosomes and tazarotene for diabetic wound repair. The patch demonstrated therapeutic effects by promoting cell migration and angiogenesis.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Oncology
Fang Huang, Zhichao Li, Wenhao Zhang, Jiaqi Li, Siguo Hao
Summary: Cell-released nanovesicles, or exosomes, derived from leukemia cells can induce anti-leukemia immunity. However, the presence of immunosuppressive PD-L1 proteins in these exosomes reduces the potency of exosome-based vaccines. In this study, researchers successfully downregulated PD-L1 expression in leukemia cells and exosomes using lentivirus-mediated PD-L1 shRNA. The resulting exosomes, termed LEXPD-L1si, showed enhanced immune effects compared to non-modified exosomes, including increased DC maturation, T cell activation, T cell proliferation, and cytokine release. Vaccination with LEXPD-L1si effectively inhibited tumor growth and prolonged survival in mice. This study highlights the potential application of optimizing exosome-based vaccines for leukemia immunotherapy.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2022)
Article
Chemistry, Medicinal
Chengliang Sun, Mingxiao Yin, Yao Cheng, Zean Kuang, Xiaojia Liu, Gefei Wang, Xiao Wang, Kai Yuan, Wenjian Min, Jingwen Dong, Yi Hou, Lingrong Hu, Guoyu Zhang, Wenli Pei, Liping Wang, Yanze Sun, Xinmiao Yu, Yibei Xiao, Hongbin Deng, Peng Yang
Summary: S4-1 is an innovative small-molecule inhibitor of PD-L1 that effectively alters the PD-L1/PD-1 interaction, enhances cytotoxicity of immune cells towards tumor cells, and shows significant tumor growth inhibition in vivo. It also activates T-cells and reverses the inhibitory tumor microenvironment.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Dermatology
Anais Zanella, Valentin Vautrot, Francois Aubin, Laure Avoscan, Mahtab Samimi, Carmen Garrido, Jessica Gobbo, Charlee Nardin
Summary: Exosomes, as potential biomarkers, have attracted interest in oncology. PD-L1 was found in circulating exosomes of MCC patients and tended to be higher in advanced disease. However, Exo-PD-L1 levels were similar to healthy donors and lower than other cancers. Changes in Exo-PD-L1 levels were not significant over the course of the disease.
EXPERIMENTAL DERMATOLOGY
(2022)
Article
Health Care Sciences & Services
Andrea Palicelli, Martina Bonacini, Stefania Croci, Alessandra Bisagni, Eleonora Zanetti, Dario De Biase, Francesca Sanguedolce, Moira Ragazzi, Magda Zanelli, Alcides Chaux, Sofia Canete-Portillo, Maria Paola Bonasoni, Stefano Ascani, Antonio De Leo, Jatin Gandhi, Alessandro Tafuni, Beatrice Melli
Summary: Liquid biopsy is a valuable tool for monitoring PD-L1 expression in PC patients, with potential correlations to prognosis and treatment efficacy. The discrepancies between primary and metastatic PC tissue biopsies, as well as between CTCs and tumor tissues, indicate the need for further research in larger sample sizes to determine the clinical implications of PD-L1 expression levels.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Medicine, General & Internal
Xuelian Chen, Fang Cheng, Yanfang Liu, Lirong Zhang, Lian Song, Xiaojie Cai, Tao You, Xin Fan, Dongqing Wang, Aihua Gong, Haitao Zhu
Article
Engineering, Biomedical
Haitao Zhu, Xiongfeng Cao, Xiaojie Cai, Ying Tian, Dongqing Wang, Jianchen Qi, Zhaogang Teng, Guangming Lu, Qianqian Ni, Shouju Wang, Longjiang Zhang
Article
Biochemistry & Molecular Biology
Kang Wang, Zhengyang Zhang, Hsiang-i Tsai, Yanfang Liu, Jie Gao, Ming Wang, Lian Song, Xiongfeng Cao, Zhanxue Xu, Hongbo Chen, Aihua Gong, Dongqing Wang, Fang Cheng, Haitao Zhu
Summary: Ferroptosis, a form of iron-dependent cell death, has been implicated as a potential target for cancer therapy. The identification of BCAT2 as a regulator of ferroptosis suggests a novel therapeutic strategy for overcoming cancer resistance. Targeting BCAT2 may hold promise for enhancing the effectiveness of ferroptosis-inducing therapies in cancer treatment.
CELL DEATH AND DIFFERENTIATION
(2021)