期刊
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
卷 63, 期 5, 页码 727-733出版社
WILEY
DOI: 10.1002/bab.1418
关键词
Clostridium beijerinckii; ferulic acid; fermentation; high-throughput screening; non-detoxified hemicellulosic hydrolysate
资金
- National Basic Research Program of China (973 Program) [2013CB733602]
- Ministry of Science and Technology of China [2014EG111227]
- National Natural Science Foundation of China [21306032]
- National High-Tech Research and Development Program of China (863) [2012AA021200]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Postdoctoral Science Foundation of Jiangsu Province [1302107C]
- Research Foundation of the Guangzhou Sugarcane Industry Research Institute [A201302]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT_14R28]
A mutant strain of Clostridium beijerinckii, with high tolerance to ferulic acid, was generated using atmospheric pressure glow discharge and high-throughput screening of C. beijerinckii NCIMB 8052. The mutant strain M11 produced 7.24g/L of butanol when grown in P2 medium containing 30g/L of glucose and 0.5g/L of ferulic acid, which is comparable to the production from non-ferulic acid cultures (8.11g/L of butanol). When 0.8g/L of ferulic acid was introduced into the P2 medium, C. beijerinckii M11 grew well and produced 4.91g/L of butanol. Both cell growth and butanol production of C. beijerinckii M11 were seriously inhibited when 0.9g/L of ferulic acid was added into the P2 medium. Furthermore, C. beijerinckii M11 could produce 6.13g/L of butanol using non-detoxified hemicellulosic hydrolysate from diluted sulfuric acid-treated corn fiber (SAHHC) as the carbon source. These results demonstrate that C. beijerinckii M11 has a high ferulic acid tolerance and is able to use non-detoxified SAHHC for butanol production. (C) 2015 International Union of Biochemistry and Molecular Biology, Inc.
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