期刊
CARBOHYDRATE POLYMERS
卷 149, 期 -, 页码 112-120出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.04.093
关键词
Trichoderma pseudokoningii; Exopolysaccharide; Immunomodulation; NF-kappa B; MAPKs; Macrophages
资金
- National Natural Science Foundation of China [81370983, 81400864, 81500692]
- Anhui Provincial Natural Science Foundation [1408085MH197, 1508085MH191]
- Key Program for the Excellent Young Talents in College of Anhui Province [2013SQRL054ZD]
- Foundation of National Key Scientific Instrument and Equipment Development Projects [2011YQ0301241403]
- Hunan Province Natural Science Key Fund Project [2014SK2003]
- Natural Science Foundation of Education Department of Anhui Province [KJ2015A199, KJ2016A723]
- Scientific Research Project of Wannan Medical College [WK201517]
In this study, we evaluated the immunomodulatory activity of an exopolysaccharide (EPS) derived from Trichoderma pseudokoningii and investigated the molecular mechanism of EPS-mediated activation of macrophages. Results revealed that EPS could significantly induce the production of nitric oxide (NO), tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta and enhance phagocytic activity in RAW 264.7 cells. Immunofluorescence staining indicated that EPS promoted the nuclear translocation of nuclear factor (NF)-kappa B p65 subunit. Western blot analysis showed that EPS increased the expression of inducible nitric oxide synthase (iNOS) protein, the degradation of I kappa B-alpha and the phosphorylation of mitogen-activated protein kinases (MAPKs). Furthermore, pretreatment of RAW 264.7 cells with specific inhibitors of NF-kappa B and MAPKs significantly attenuated EPS-induced TNF-alpha and IL-1 beta production. EPS also induced the inhibition of cytokine secretion by special antibodies against Toll-like receptor-4 (TLR4) and Dectin-1. These data suggest that EPS from Trichoderma pseudokoningii activates RAW 264.7 cells through NF-kappa B and MAPKs signaling pathways via TLR4 and Dectin-1. (C) 2016 Elsevier Ltd. All rights reserved.
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