期刊
CARBOHYDRATE POLYMERS
卷 147, 期 -, 页码 354-364出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.03.092
关键词
Chickpea hull; Cicer arietinum L; Polysaccharide extraction; Box-Behnken design; In vitro antioxidant activity
资金
- National Natural Science Foundation of China [31201454]
- Specialized Research Fund for the Doctoral Program of Higher Education [20130097110021]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
The optimal extraction conditions with a yield of 5.37 +/- 0.15% for extraction of polysaccharides from chickpea (Cicer arietinum L.) hull (CHPS) were determined as extraction temperature 99 degrees C, extraction time 2.8 h and ratio of water to raw material 24 mL/g. Three fractions of CHPS-1, CHPS-2 and CHPS-3, with average molecular weight of 3.1 x 10(6), 1.5 x 10(6) and 7.8 x 10(5) Da, respectively, were obtained from crude CHPS by chromatography of DEAE Fast Flow and Sephadex G-100. CHPS-1 was composed of man nose, rhamnose, galactose, galacturonic acid, glucose and arabinose, CHPS-2 was composed of mannose, rhamnose, galacturonic acid, galactose, xylose and arabinose, CHPS-3 was composed of galacturonic acid, galactose and rhamnose. CHPS-3 showed the strongest reducing power and protective effect on H2O2-induced oxidative injury in PC12 cells and highest scavenging activities against DPPH and ABTS radicals, while CHPS-2 showed the highest scavenging activity against superoxide anion radical. (C) 2016 Elsevier Ltd. All rights reserved.
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