Article
Biochemistry & Molecular Biology
Noor Hidayah Abd Rahman, Roshanida A. Rahman, Zaidah Rahmat, Nardiah Rizwana Jaafar, Ni Nyoman Tri Puspaningsih, Rosli Md Illias
Summary: Cross-linked pectinase aggregates (CLPA) provide better operational stability and reusability for the production of pecticoligosaccharides (POS) from pectin.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Chemistry, Applied
Somnath Basak, Uday S. Annapure
Summary: Chemical methods can be used to modify hydrocolloids to alter their functional properties, but there is a recent trend towards adopting eco-friendly and green methods. Pectin, an important hydrocolloid, is widely used in food applications for its gelling, emulsifying, and stabilizing properties. Green methods such as microwave and pulsed electric field can alter the properties of pectin and make it more suitable for use in food products. Pectic oligosaccharides produced using novel approaches are being explored for their biological properties and incorporation in functional foods.
CARBOHYDRATE POLYMERS
(2022)
Article
Biochemistry & Molecular Biology
Shengnan Yu, Haoyu Wang, Luwen Cui, Jingyi Wang, Zixuan Zhang, Zhinan Wu, Xiaoqian Lin, Ningning He, Yuanqiang Zou, Shangyong Li
Summary: Accumulating evidence has shown that pectin oligosaccharides (POS) derived from pectin degradation can prevent and alleviate obesity by regulating the gut microbiota. POS supplementation in mice fed a high-fat diet (HFD) resulted in decreased body weight, improved glucose tolerance, reduced fat accumulation and hepatic steatosis, protected intestinal barrier, and reduced pro-inflammatory cytokine levels. Fecal metagenomic sequencing revealed that POS corrected the gut microbiota dysbiosis caused by HFD, promoting populations of beneficial bacteria and reducing harmful bacteria.
Article
Chemistry, Applied
Linlin Li, Ziqian Li, Thomas Balle, Guoqin Liu, Zheng Guo
Summary: This study synthesized a new multipurpose amphiphilic POS by coupling depolymerized citrus pectin with alkyl succinylation. The resulting POS showed excellent performance in stabilizing oil/water emulsions and other extensive applications.
FOOD HYDROCOLLOIDS
(2023)
Review
Food Science & Technology
Somnath Basak, Uday S. Annapure
Summary: Subcritical water extraction of pectin preserves the hairy region and has high selectivity, while subcritical water can aid in the breakdown of pectic polysaccharides into oligosaccharides. The extracted pectin exhibits better bioactive and emulsifying properties, but negatively affects rheological properties. Organic acids in subcritical water have a similar action as enzymes in hydrolyzing pectic polysaccharides into fragments.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Chemistry, Applied
Haixia Yan, Xiaoyu Zhang, Liu Yang, Yixiao Shen, Ling Liu
Summary: This study characterized the structure of pectic oligosaccharides of orange peel (OPOs) and found that they have a high level of anti-glycation activity. The main component of OPOs showed a strong inhibition of glycation at moderate temperature and this inhibition was related to its composition and time.
Article
Green & Sustainable Science & Technology
Sheng Wang, Sunjia Ding, Kun Meng, Xiaoqing Liu, Yuan Wang, Xiaolu Wang, Xing Qin, Huiying Luo, Bin Yao, Huoqing Huang, Tao Tu
Summary: This study aimed to produce methyl-esterified pectin oligosaccharides (POS) for the resource utilization of agricultural waste. The underlying synergistic effects of the dual-catalytic domain within bifunctional S6A were investigated, and the enzyme was applied to produce methyl-esterified POS. The antimicrobial activities of the produced POS were evaluated, indicating the potential of bifunctional S6A to produce antibacterial POS from agricultural waste.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Food Science & Technology
Malaiporn Wongkaew, Bow Tinpovong, Korawan Sringarm, Noppol Leksawasdi, Kittisak Jantanasakulwong, Pornchai Rachtanapun, Prasert Hanmoungjai, Sarana Rose Sommano
Summary: Pectin recovery from mango peel biomass can be a potential source of pectic oligosaccharides with probiotic growth-enhancing properties. The optimal hydrolysis treatment of mango peel pectin can improve the prebiotic effects of selected bacteria and provide a sustainable solution for utilizing by-products from the fruit industry.
Article
Food Science & Technology
Lin Xue, Jie Long, Cheng Lu, Xingfei Li, Xueming Xu, Zhengyu Jin
Summary: The study demonstrates that immobilized polygalacturonase through sol-gel entrapment shows high catalytic activity and stability, making it suitable for continuous preparation of pectic oligosaccharides with good antibacterial properties.
Article
Food Science & Technology
Jie Long, Xingfei Li, Lin Xue, Zhengjun Xie, Aiquan Jiao, Yuxiang Bai, Xing Zhou, Long Chen, Chao Qiu, Xueming Xu, Zhengyu Jin
Summary: This study aimed to continuously hydrolyze mango peel pectin into pectic oligosaccharides (POS) with antibacterial activity. A packed-bed reactor with immobilized polygalacturonase was designed and optimized using response surface analysis. Stable POS production with a yield of 94.56% was achieved, and the reactor retained 82% of its initial activity after continuous operation for 72 hours. The POS products exhibited good antibacterial activity against various bacteria and demonstrated destructive effects on membrane integrity.
Article
Food Science & Technology
Sudarat Thikham, Sitthidat Tongdonyod, Chatchawan Kantala, Phatthanaphong Therdtatha, Wannaporn Klangpetch
Summary: This study aims to valorize orange juice by-products by producing crude pectic oligosaccharides with prebiotic potential using pulsed electric field-assisted enzymatic treatment. The results showed that this method achieved high yields of the crude products, which can enhance the growth of probiotic bacteria and produce short-chain fatty acids.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Article
Chemistry, Multidisciplinary
Stefania Adelina Milea, Oana Craciunescu, Gabriela Rapeanu, Anca Oancea, Elena Enachi, Gabriela Elena Bahrim, Nicoleta Stanciuc
Summary: The study developed a sustainable solution for managing onion skin by-products, focusing on designing ingredients with multifunctional activities and utilizing green solvent aqueous extraction. The resulting powders showed satisfactory phytochemical content, good stability during storage, and compatibility with cell proliferation. Furthermore, confocal microscopy revealed the distribution of bioactive compounds within the powders' biopolymeric matrices, and in vitro digestion showed stable release and controlled release of flavonoids in the intestinal phase.
APPLIED SCIENCES-BASEL
(2021)
Review
Biochemistry & Molecular Biology
Sergio Martinez-Gomez, Marcos Fernandez-Bautista, Sandra Rivas, Remedios Yanez, Jose L. Alonso
Summary: The aging population is associated with various health issues, and diet plays a crucial role in the development of these diseases. Functional ingredients, particularly prebiotics, have been extensively studied for their potential benefits in preventing or treating these diseases.
Article
Biotechnology & Applied Microbiology
Francesca Anna Ramires, Anna Rita Bavaro, Isabella D'Antuono, Vito Linsalata, Leone D'Amico, Federico Baruzzi, Loris Pinto, Annamaria Tarantini, Antonella Garbetta, Angela Cardinali, Gianluca Bleve
Summary: Onion skins, recycled as organic fertilizers, can be used as a substrate in environmentally friendly bioprocesses to recover valuable bioactive compounds and food ingredients. A bioprospecting method was employed to examine the growth ability of bacterial and yeast strains on onion skins as a unique nutrient source. Fermented onion skins were also investigated as an affordable source of microbial enzymes.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Tianlu Li, Youqin Zhang, Tong Li, Haoru Zhuang, Fengshan Wang, Ning Wang, Richard R. Schmidt, Peng Peng
Summary: O-Mannosylation is crucial for various biological processes, but its precise function is still largely unknown. This study developed a chemoenzymatic strategy to assemble diverse O-mannosyl alpha-dystroglycan (alpha-DG) glycopeptides efficiently, demonstrating its effectiveness and flexibility.
CHINESE JOURNAL OF CHEMISTRY
(2022)
Article
Engineering, Chemical
Alexandra Bouriakova, Pedro S. F. Mendes, Kathy Elst, Jeriffa De Clercq, Joris W. Thybaut
CHEMICAL ENGINEERING RESEARCH & DESIGN
(2020)
Review
Food Science & Technology
Jose Villacis-Chiriboga, Kathy Elst, John Van Camp, Edwin Vera, Jenny Ruales
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2020)
Article
Biochemical Research Methods
Barbara Prandi, Francesca Lambertini, Martina Varani, Andrea Faccini, Michele Suman, Andrea Leporati, Tullia Tedeschi, Stefano Sforza
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2020)
Article
Multidisciplinary Sciences
Giulia Leni, Tullia Tedeschi, Andrea Faccini, Federico Pratesi, Claudia Folli, Ilaria Puxeddu, Paola Migliorini, Natasja Gianotten, Johan Jacobs, Stefaan Depraetere, Augusta Caligiani, Stefano Sforza
SCIENTIFIC REPORTS
(2020)
Article
Biotechnology & Applied Microbiology
Guneet Kaur, Linsey Garcia-Gonzalez, Kathy Elst, Federica Truzzi, Lorenzo Bertin, Ankita Kaushik, Malini Balakrishnan, Heleen De Wever
BIOCHEMICAL ENGINEERING JOURNAL
(2020)
Article
Chemistry, Applied
Giulia Leni, Barbara Prandi, Martina Varani, Andrea Faccini, Augusta Caligiani, Stefano Sforza
Article
Agronomy
Francesca Taranto, Nunzio D'Agostino, Marcello Catellani, Luca Laviano, Domenico Ronga, Justyna Milc, Barbara Prandi, Fatma Boukid, Stefano Sforza, Sara Graziano, Mariolina Gulli, Giovanna Visioli, Nelson Marmiroli, Franz-W. Badeck, Anna Paola Minervini, Ivano Pecorella, Nicola Pecchioni, Pasquale De Vita, Enrico Francia
Article
Nutrition & Dietetics
Federica Gaiani, Sara Graziano, Fatma Boukid, Barbara Prandi, Lorena Bottarelli, Amelia Barilli, Arnaldo Dossena, Nelson Marmiroli, Mariolina Gulli, Gian Luigi de'Angelis, Stefano Sforza
Article
Biochemistry & Molecular Biology
Sandra Corderi, Tom Renders, Kelly Servaes, Karolien Vanbroekhoven, Tony De Roo, Kathy Elst
Summary: The study presents two simple methods for removing polysaccharides from biorefinery lignin samples, which can reduce the polysaccharide content of lignin and have economic potential for the production of bio-based chemicals and materials.
Article
Food Science & Technology
Jose Villacis-Chiriboga, Stefan Voorspoels, Maarten Uyttebroek, Jenny Ruales, John Van Camp, Edwin Vera, Kathy Elst
Summary: The potential of supercritical CO2 for extracting bioactive compounds from mango by-products was evaluated using a design of experiments. Optimal conditions were found for carotenoid extraction, with the peel fraction showing significantly higher bioactive content compared to the pulp fraction. Supercritical CO2 is a promising technology for isolating valuable compounds from mango by-products.
Article
Biochemistry & Molecular Biology
Alessandra Gasparini, Sara Benede, Tullia Tedeschi, Stefano Sforza, Isidra Recio, Beatriz Miralles
Summary: This study evaluated the effects of thermal processing and lactosylation on the digestibility and allergenicity of whey proteins. The industrially treated sample exhibited faster gastric digestion and lower immunogenic response compared to the raw sample. Heat treatment resulted in the release of linear epitopes, leading to higher IgE reactivity. The immunogenic response at the intestinal level was negligible.
Article
Biochemistry & Molecular Biology
Barbara Prandi, Maura Ferri, Stefania Monari, Chiara Zurlini, Ilaria Cigognini, Stefanie Verstringe, Dennis Schaller, Martha Walter, Luciano Navarini, Annalisa Tassoni, Stefano Sforza, Tullia Tedeschi
Summary: This study conducted a detailed chemical analysis of non-compliant green coffee beans and coffee silverskins, revealing that green coffee beans contain higher levels of polyphenols, flavanols, flavonoids, and caffeine, while silver skins are richer in tannins. Enzyme-assisted extraction yielded extracts with good protein content and consistent polyphenol quantities, showing antioxidant, antimicrobial, and antityrosinase activities. The antioxidant and antimicrobial activities of green coffee bean extracts were superior to those of coffee silver skin extracts, with comparable antityrosinase activity.
Article
Engineering, Environmental
Deepak Raikwar, Korneel Van Aelst, Thijs Vangeel, Sandra Corderi, Joost Van Aelst, Sander Van den Bosch, Kelly Servaes, Karolien Vanbroekhoven, Kathy Elst, Bert F. Sels
Summary: Unsatisfactory solubility and recalcitrant condensed structures of isolated lignins (technical lignins) severely limit their processability. Understanding the structural and physicochemical properties of isolated lignins is crucial for explaining their performance in solubilization and further depolymerization. This study investigated the solubilization and depolymerization of organosolv lignins from three different sources. Solubility tests were conducted in six organic solvents, and solubilization behavior was described based on structural properties and investigated under different conditions. Strong structural correlations between parent lignin/subfractions and depolymerized lignin oils were derived.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Food Science & Technology
Jose Villacis-Chiriboga, Barbara Prandi, Jenny Ruales, John Van Camp, Stefano Sforza, Kathy Elst
Summary: This research investigated the valorization of soursop seeds to produce oil and proteins. A new mild protein extraction method (Pressurized Water Extraction, PWE) was developed and optimized, and compared with conventional alkaline extraction. The impact of the de-oiling technique was also investigated. The extracted proteins were evaluated in terms of physicochemical composition, amino acid content, and techno-functional properties.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Barbara Prandi, Fatma Boukid, Simon Van De Walle, Sara Cutroneo, Josep Comaposada, Geert Van Royen, Stefano Sforza, Tullia Tedeschi, Massimo Castellari
Summary: The addition of microalgae to vegetable creams improved the protein content and the amino acid nutritional profile of vegetable creams, but had no significant effect on protein digestibility. This study suggests that incorporating microalgae is a feasible strategy to increase the protein content and nutritional quality of foods.
Review
Chemistry, Applied
Xingran Kou, Dongdong Su, Fei Pan, Xiwei Xu, Qingran Meng, Qinfei Ke
Summary: This review provides a systematic discussion of the application of molecular dynamics (MD) simulations in aroma compounds (ACs)/cyclodextrins (CDs) inclusion complexes (ICs). It covers the establishment of the simulation process, parameter selection, model evaluation, and various application cases, summarizing the major achievements and challenges of this method.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Haoyu Yu, Quanbin Zhang, Ammad Ahmad Farooqi, Jing Wang, Yang Yue, Lihua Geng, Ning Wu
Summary: Brown algae are rich in fucoidan, which has been found to have anti-cancer and anti-metastasis effects. Fucoidan inhibits tumor cell growth, proliferation, and metastasis, and also promotes immune responses in the tumor microenvironment.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Liang Dong, Yanan Li, Hailin Cong, Bing Yu, Youqing Shen
Summary: Gene therapy is a revolutionary treatment that requires suitable vectors for protecting and releasing exogenous nucleic acids in target cells. Chitosan, as a non-viral vector, has gained attention due to its good biocompatibility and ability to load large amounts of nucleic acids. This paper summarizes the potential of chitosan and its derivatives as gene delivery vector materials, discusses factors influencing transfection efficiency, performance evaluation, ways to optimize infectious efficiency, and current research development directions. It also provides an outlook on the future prospects of chitosan.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ana Iglesias-Mejuto, Beatriz Magarinos, Tania Ferreira-Goncalves, Ricardo Starbird-Perez, Carmen Alvarez-Lorenzo, Catarina Pinto Reis, Ines Ardao, Carlos A. Garcia-Gonzalez
Summary: This study developed a novel processing strategy to manufacture drug-loaded and personalized aerogels with nanostructures. The aerogels demonstrated bioactivity and antimicrobial effects, promoting bone regeneration and preventing infections in bone tissue engineering.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Zhenxu Shi, Dimei Yang, Yan Zhou, Xinyu Chen, Lin Gan, Jin Huang
Summary: This study proposes a micro-assembly method to improve the photoluminescent properties of crystalline cellulose nanocrystals (CNCs) by organizing them within a sub-micrometer-sized metal-organic framework and coating with TiO2. The TiO2 coating prevents CNC assembly breakdown and allows information to be revealed using screenprinted labels for anti-counterfeiting purposes.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Xuejiao Liu, Bin Fu, Jierong Chen, Zhenyu Sun, Dongdong Zheng, Zhonghua Li, Bing Gu, Ying Zhang, Haojie Lu
Summary: Liver disease is a major cause of global mortality, and identifying biomarkers for diagnosing its progression is crucial for improving outcomes. Targeted mass spectrometry is a powerful tool for verifying biomarker candidates and clinical applications, particularly for glycoproteins translation. However, the limitation of analyzing only one sample per run has become apparent. In this study, a high-throughput intact N-glycopeptides quantification strategy was developed, allowing the validation of 20 samples per run with an average analysis time of 3 minutes per sample. The strategy was applied in a cohort of 461 serum samples and identified a panel of 10 IgG N-glycopeptides that have strong clinical utility in evaluating the severity of liver disease.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Qian Wang, Hao Jiang, Hongli Zhang, Weiqiao Lu, Xiao Wang, Wenfeng Xu, Jia Li, Youjing Lv, Guoyun Li, Chao Cai, Guangli Yu
Summary: This study proposes a novel strategy of antibody-beta-glucan conjugates (AGC) to enhance the antitumor immune response to immune checkpoint blockade (ICB) therapy. AGC demonstrated powerful tumor suppression and promoted interaction between tumor cells and dendritic cells (DCs), thereby enhancing immunotherapeutic benefits.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Chaeyeon Lee, Sojeong Lee, Sang Wook Kang
Summary: The study aims to enhance the properties of porous membranes by addressing the limitations associated with phase separation. By using cellulose acetate and citric acid, the researchers were able to fabricate membranes with improved mechanical strength and thermal stability. The cross-linking effect of citric acid resulted in a more uniform pore structure and higher porosity.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Tejaswini Kolipaka, Giriraj Pandey, Noella Abraham, Dadi A. Srinivasarao, Rajeev Singh Raghuvanshi, P. S. Rajinikanth, Vidya Tickoo, Saurabh Srivastava
Summary: This review focuses on the design and application of polysaccharide-based hydrogel wound dressings, highlighting aspects such as biocompatibility, biodegradability, drug entrapment, moisturizing ability, swelling, and mechanical properties. Additionally, various crosslinking methods and recent developments in stimuli-responsive hydrogels are discussed.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Anqi Xu, Nan Zhang, Shixing Su, Hongyu Shi, Daoqiang Lu, Xifeng Li, Xin Zhang, Xin Feng, Zhuohua Wen, Gengwu Ma, Mengshi Huang, Chi Huang, Yuqi Hu, Hao Yuan, Qinwen Liu, Daogang Guan, Jun Wang, Chuanzhi Duan
Summary: The study presents a one-pot radical polymerization method to fabricate a hydrogel with adhesive properties, which can effectively treat bacterial-infected diabetic wounds and accelerate wound healing.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Montarop Yamabhai, Munthipha Khamphio, Thae Thae Min, Chai Noy Soem, Nguyen Cao Cuong, Waheni Rizki Aprilia, Krisanai Luesukprasert, Karsidete Teeranitayatarn, Atthaphon Maneedaeng, Tina R. Tuveng, Silje B. Lorentzen, Simen Antonsen, Paiboon Jitprasertwong, Vincent G. H. Eijsink
Summary: The study investigates the bioconversion of chitosan into soluble anti-inflammatory chitosan oligosaccharides (CHOS) using an enzyme. The results show that the generated CHOS have anti-inflammatory activity, but the magnitude of the activity depends on the substrate and production process. Different methods of dissolving chitosan also affect the properties of CHOS. The study highlights the importance of quality assurance in CHOS preparations.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Wen Cao, Jiangping Wu, Xinya Zhao, Zixu Li, Jie Yu, Taili Shao, Xuefeng Hou, Lutan Zhou, Chunfei Wang, Guodong Wang, Jun Han
Summary: In this study, a water-soluble polysaccharide (PLP1) was successfully isolated and purified from Pueraria lobata. It was found that PLP1 was composed of specific glycosidic units and exhibited a better free radical-scavenging ability. Moreover, PLP1 effectively protected the liver against acute alcoholic liver disease (ALD) in mice.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ya Su, Yaqian Liu, Xueyan Hu, Yueqi Lu, Jinyuan Zhang, Wenbo Jin, Wang Liu, Yan Shu, Yuen Yee Cheng, Wenfang Li, Yi Nie, Bo Pan, Kedong Song
Summary: A 3D printed scaffold based on carbon dots-curcumin nano-drug release has been developed for drug delivery after breast cancer surgery. The scaffold showed effective inhibition of tumor growth, antibacterial activity, and promotion of wound healing, making it a promising approach for preventing tumor recurrence.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ekaterina Sokolova, Diane Jouanneau, Antonin Chevenier, Murielle Jam, Nathalie Desban, Pierre Colas, Elizabeth Ficko-Blean, Gurvan Michel
Summary: Carrageenans, a compound synthesized in red algae, have various biological properties and are valuable in the pharmaceutical and cosmetic industries. Their fine structure affects wound healing, oxidative processes, hemostasis, and inflammation. Enzymatic modification of carrageenans produces oligosaccharides that bind to natural human serum antibodies and specific antibodies, showing potential for therapeutic applications.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Yan Shi, Miao Yu, Kaijin Qiu, Tiantian Kong, Chunjing Guo, Wenxue Zhang, Daquan Chen, Ming Kong
Summary: In this study, functionalized transfersomes were developed to co-deliver doxorubicin and 1MT towards primary tumors and tumor draining lymph nodes via transdermal administration using microneedles. The results showed that the nano-complexed microneedles exhibited a stronger suppression in tumor growth compared to the intravenous group.
CARBOHYDRATE POLYMERS
(2024)
