Article
Engineering, Biomedical
William Hardiman, Matt Clark, Claire Friel, Alan Huett, Fernando Perez-Cota, Kerry Setchfield, Amanda J. Wright, Manlio Tassieri
Summary: Microrheology is a study of fluid properties on the micron length-scales, which can provide insights into cellular biology and biomechanics. In this study, a minimally-invasive passive microrheology technique is used to investigate the mechanical properties of individual living cells. The results show changes in the cells' low-frequency elastic modulus and dynamics over a range of timescales.
ACTA BIOMATERIALIA
(2023)
Article
Physiology
Claudia Bernecker, Maria Lima, Tatjana Kolesnik, Annika Lampl, Catalin Ciubotaru, Riccardo Leita, Dagmar Kolb, Eleonore Froehlich, Peter Schlenke, Gerhard A. Holzapfel, Isabel Dorn, Dan Cojoc
Summary: Modern medicine has an increasing demand for safe blood products, and ex vivo cultured red blood cells (cRBC) are seen as a potential standardized and safe source. This study aimed to compare the topographical and biomechanical properties of cRBC with native reticulocytes (nRET) and erythrocytes (nRBC). The combination of different methods, including optical tweezers, atomic force microscopy, and digital holographic microscopy, was used to obtain comprehensive information. The results showed a high similarity between cRBC and nRBC in terms of imaging, deformability, recovery time, Young's modulus, and plasticity index. Lipid deficiency affected the shape and biomechanical functionality of cRBC. The study highlights the importance of lipid content in the shape and biomechanical properties of red blood cells.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Chemistry, Analytical
Riccardo Tognato, Philip H. Jones
Summary: In this paper, the trapping of healthy red blood cells in optical tweezers is numerically studied and it is found that the biconcave shape of the cells significantly affects the optical forces and torques. It is also demonstrated that adding an additional laser beam can control the orientation of the trapped cells. This investigation sheds light on the trapping mechanism of healthy erythrocytes and provides guidance for designing new experiments.
Review
Biochemical Research Methods
Yanzheng Xie, Xiaoshuai Liu
Summary: Red blood cells serve as natural vehicles to deliver oxygen in the body and are important indicators for biomedical analysis. Optical tweezers provide a flexible tool for precise manipulation and quantitative characterization of RBCs' physiological mechanisms and viscoelastic properties.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Chemistry, Analytical
Pavel Yale, Michel A. Kouacou, Jean-Michel E. Konin, Eugene Megnassan, Jeremie T. Zoueu
Summary: This paper investigates the lateral deformation and elastic stiffness of human red blood cells (RBCs) when laterally indented by an optically trapped silica bead. It demonstrates the importance of considering lateral force and cell-bead interaction during the experiment. The results show that this approach can be used as a routine method for studying RBCs.
Article
Physics, Multidisciplinary
Andrew B. Matheson, Tania Mendonca, Graham M. Gibson, Paul A. Dalgarno, Amanda J. Wright, Lynn Paterson, Manlio Tassieri
Summary: This study investigated the effect of anisotropic optical traps on microrheology measurements and found that using highly anisotropic optical traps for viscosity measurements will lead to erroneous results. It introduced a new analytical approach for anisotropic traps with two axes of symmetry and identified a threshold level of anisotropy in optical trap strength. The outcomes of this study may have important practical ramifications on how all microrheology measurements should be conducted and analyzed in the future.
FRONTIERS IN PHYSICS
(2021)
Article
Multidisciplinary Sciences
Andrew B. Matheson, Lynn Paterson, Amanda J. Wright, Tania Mendonca, Manlio Tassieri, Paul A. Dalgarno
Summary: The novel 3D microrheology system OpTIMuM integrates Optical Tweezers with Integrated Multiplane Microscopy for the first time, allowing the precise tracking of optically trapped beads with around 20 nm accuracy along the optical axis. This system does not require a high precision z-stage or separate calibration sample, and features a simple yet effective in situ spatial calibration method using image sharpness. We demonstrated its effectiveness by measuring the viscosity of water in three dimensions simultaneously.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Claudia Bernecker, Maria Augusta R. B. F. Lima, Catalin D. Ciubotaru, Peter Schlenke, Isabel Dorn, Dan Cojoc
Summary: This study introduces a novel optical tweezer approach to measure the deformation and elasticity of single red blood cells, providing new insights into the biomechanical characterization of cultured red blood cells. The results show that red blood cells cultured with human platelet lysate exhibit similar features to native red blood cells under optical forces, while those cultured with plasma display different characteristics.
Article
Multidisciplinary Sciences
Anna Schepers, Charlotta Lorenz, Peter Nietmann, Andreas Janshoff, Stefan Klumpp, Sarah Koster
Summary: The mechanical properties of cells are largely determined by the cytoskeleton, with the intermediate filament network being the most extensible and stress-resilient. A multiscale approach is used to analyze the contributions of single-filament mechanics, filament length, and interactions between filaments to vimentin IF network mechanics. Hydrophobic contributions primarily affect filament elongation kinetics, while electrostatics influence filament-filament interactions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Ruixue Zhu, Tatiana Avsievich, Xinyang Su, Alexander Bykov, Alexey Popov, Igor Meglinski
Summary: Proper rheological properties of red blood cells (RBC), including flexibility and aggregability, are crucial for healthy blood microcirculation. This study found that low-level laser radiation has positive effects on RBC, improving their deformability and weakening the strength of cell-cell interaction.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2022)
Review
Cell Biology
Shin-Jeong Lee, Cholomi Jung, Jee Eun Oh, Sangsung Kim, Sangho Lee, Ji Yoon Lee, Young-sup Yoon
Summary: Red blood cell (RBC) transfusion is a lifesaving procedure for anemia and hemoglobin disorders, but faces challenges due to the shortage of blood supply and risks of infection and incompatibility. The use of human pluripotent stem cells (hPSCs), including human induced pluripotent stem cells (hiPSCs), holds promise for generating RBCs. This review discusses the concepts and mechanisms of erythropoiesis, methodologies for differentiating hPSCs into erythrocytes, and the limitations and future directions of using hiPSC-derived erythrocytes in clinical applications.
Article
Physics, Multidisciplinary
Felix Ginot, Juliana Caspers, Luis Frieder Reinalter, Karthika Krishna Kumar, Matthias Krueger, Clemens Bechinger
Summary: The research investigates the transient recoil dynamics of a colloidal probe particle in a viscoelastic fluid, displaying two distinct timescales that agree with a microscopic model. The model reveals two sets of eigenmodes which correspond to reciprocal and non-reciprocal force conditions, confirming in experiments. The findings are expected to be relevant in conditions where particles are exposed to non-steady shear forces.
NEW JOURNAL OF PHYSICS
(2022)
Review
Immunology
Timothy J. Satchwell
Summary: This review discusses the methodologies of generating red blood cells susceptible to malaria parasite invasion through in vitro erythroid differentiation of stem cells, and explores the potential of in vitro derived red blood cells for malaria research.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Sandrine Laurance, Mickael Marin, Yves Colin
Summary: Central retinal vein occlusion (CRVO) is a common retinal disorder leading to blindness, but the primary cause and treatment remain unidentified. This review aims to provide an overview of CRVO pathophysiology, specifically focusing on the role of red blood cells (RBCs). The review explores the functions of RBCs beyond oxygen transport and their potential involvement in CRVO occurrence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Shufang Wang, Guibin Wang, Shichun Lu, Jiaying Zhang, Wenwen Zhang, Yuanyuan Han, Xiaoyu Cai, Yuan Zhuang, Fei Pu, Xin Yan, Zhiwei Tu, Liang Wang, Xirui Huang, Bin Fan, Deqing Wang, Zhaojun Zhang
Summary: This study investigates the association between red blood cells and hepatocellular carcinoma (HCC) tumorigenesis. The researchers found dynamic changes in red blood cells during HCC tumorigenesis, suggesting a potential link between immature erythroid cells and HCC. They also identified disrupted pathways and promising biomarkers for the early diagnosis of HCC. This study provides a new perspective on HCC tumorigenesis and may aid in early detection.
Article
Engineering, Biomedical
L. Trifonyuk, A. Sdobnov, W. Baranowski, V Ushenko, O. Olar, A. Dubolazov, L. Pidkamin, M. Sidor, O. Vanchuliak, A. Motrich, M. Gorsky, I Meglinski
LASERS IN MEDICAL SCIENCE
(2020)
Article
Optics
Andrey D. Bulygin, Denis A. Vrazhnov, Elena S. Sim, Igor Meglinski, Yury V. Kistenev
OPTICAL ENGINEERING
(2020)
Article
Biochemical Research Methods
Mariia Borovkova, Alexander Bykov, Alexey Popov, Igor Meglinski
JOURNAL OF BIOMEDICAL OPTICS
(2020)
Article
Biochemical Research Methods
Motahareh Peyvasteh, Alexey Popov, Alexander Bykov, Angelo Pierangelo, Tatiana Novikova, Igor Meglinski
Summary: The study explores the use of Mueller matrix imaging polarimetry for assessing meat quality, presenting promising results regarding the dynamic changes in tissue optical properties over time. The wavelength spectra of MM elements exhibit specific trends related to meat color, freshness, birefringence, and tenderness.
JOURNAL OF BIOPHOTONICS
(2021)
Article
Biophysics
Ruixue Zhu, Tatiana Avsievich, Alexey Popov, Alexander Bykov, Igor Meglinski
Summary: Biosensors based on nanotechnology have vital positions in various fields. The potential health risks of novel nano-materials lack comprehensive assessment. RBC-mediated in vivo delivery strategies have wide applications in bio-imaging, medical diagnostics, and analysis of biomolecules and cellular activities.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Engineering, Electrical & Electronic
V. V. Shupletsov, E. A. Zherebtsov, V. V. Dremin, A. P. Popov, A. Bykov, E. Potapova, A. Dunaev, I. Meglinski
Summary: A new technique has been developed for fabricating composite phantoms mimicking fluorescence properties of human skin using polymerisable polyacrylamide, collagen and an aqueous solution of FAD. Comparative analysis of optical properties of phantoms with different concentrations of FAD show good agreement between model fluorescence spectra and direct human skin measurements.
QUANTUM ELECTRONICS
(2021)
Article
Physics, Multidisciplinary
Benjamin T. Hogan, Volodimyr A. Ushenko, Anastasia-Vira Syvokorovskaya, Alexander Dubolazov, Oleg Ya Vanchulyak, Alexander G. Ushenko, Yuriy A. Ushenko, Mykhailo P. Gorsky, Yuriy Tomka, Sergey L. Kuznetsov, Alexander Bykov, Igor Meglinski
Summary: This study introduces a new method to analyze the optical anisotropy parameters of myocardial tissues, using 3D Mueller matrix technology, which could provide accurate diagnostics in heart disease research.
FRONTIERS IN PHYSICS
(2021)
Article
Computer Science, Interdisciplinary Applications
Mariia Borovkova, Oleksii Sieryi, Ivan Lopushenko, Natalia Kartashkina, Jens Pahnke, Alexander Bykov, Igor Meglinski
Summary: The minimum histological criterion for diagnosing Alzheimer's disease (AD) is the presence of senile plaques and neurofibrillary tangles in specific brain locations. We developed a multi-spectral Stokes polarimetric imaging approach that allows characterization of FFPE brain tissue samples to discern the stages of AD progression without sectioning and staining the tissue.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2022)
Article
Biochemistry & Molecular Biology
Ruixue Zhu, Tatiana Avsievich, Xinyang Su, Alexander Bykov, Alexey Popov, Igor Meglinski
Summary: Proper rheological properties of red blood cells (RBC), including flexibility and aggregability, are crucial for healthy blood microcirculation. This study found that low-level laser radiation has positive effects on RBC, improving their deformability and weakening the strength of cell-cell interaction.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Alexey Popov, Maxim Timofeyev, Alexander Bykov, Igor Meglinski
Summary: This paper presents an approach for monitoring aquatic ecosystem changes by measuring luminescence intensity to evaluate temperature-induced stress in aquatic organisms.
Article
Pharmacology & Pharmacy
Tatiana Avsievich, Ruixue Zhu, Alexey P. Popov, Alexander Yatskovskiy, Anton A. Popov, Gleb Tikhonowsky, Andrei I. Pastukhov, Sergei Klimentov, Alexander Bykov, Andrei Kabashin, Igor Meglinski
Summary: This research investigated the changes in red blood cells (RBC) induced by laser-synthesized plasmonic nanoparticles (NP) made of noble metals (Au and Ag) and nitride-based materials (TiN and ZrN). The effects observed include RBC poikilocytosis, alterations in RBC microrheological parameters, and changes in RBC deformability and intercellular interactions. The study found that nitride-based NP exhibited better biocompatibility towards RBC and higher photothermal efficiency compared to their noble metal counterparts.
Article
Physics, Applied
Chang Zhang, Xiaojian Cui, Weiwei Zhang, Ruixue Zhu, Yujun Song
Summary: Surface magneto-plasmon (SMP) sensors are highly attractive due to their enhanced signal-to-noise ratios, sensitivities, and detection limits. However, there has been limited research on SMP sensors based on films containing arrays of nanopores. In this study, a high-performance SMP-MOKE sensor based on a square array of Au-Co double-layer nanopores is proposed. The sensor demonstrates a high sensitivity of 711 nm/Refractive Index Units (RIUs) and a figure of merit (FOM) of the order of 10(5) RIU-1, which surpasses existing grating-type sensors.
JOURNAL OF APPLIED PHYSICS
(2023)
Article
Computer Science, Interdisciplinary Applications
Viktor Dremin, Zbignevs Marcinkevics, Evgeny Zherebtsov, Alexey Popov, Andris Grabovskis, Hedviga Kronberga, Kristine Geldnere, Alexander Doronin, Igor Meglinski, Alexander Bykov
Summary: Protein glycation and dysfunction of collagen-containing tissues play a significant role in the development of various pathological conditions. Through photonics-based technology and machine learning, a diagnostic approach capable of evaluating skin complications of diabetes mellitus at an early stage has been introduced, showing the ability to differentiate diabetic and control groups. The development of polarization-based hyperspectral imaging technique with artificial neural network implementation provides new perspectives in the study and diagnosis of age-related diseases.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2021)
Article
Chemistry, Physical
He Zhao, Kalyani Chordiya, Petri Leukkunen, Alexey Popov, Mousumi Upadhyay Kahaly, Krisztian Kordas, Satu Ojala
Summary: This study presents a novel approach to stabilize metal halide perovskites in water using dimethylammonium iodide (DAI), without the need for acids or coatings. The stability is achieved through a surface dissolution-recrystallization process, where the concentration of DAI and I- ions play critical roles.
