期刊
CARBOHYDRATE POLYMERS
卷 143, 期 -, 页码 131-138出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.02.017
关键词
Achillea millefolium; Polysaccharide; THP1-cells; Cytokines; MAP kinases
资金
- Icelandic Research Fund [110418021]
- University of Iceland Research Fund
- Landspitali University Hospital Research Fund
Achillea millefolium has been used in traditional medicine for a number of ailments, including skin inflammation and wounds. A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7. THP-1 cells primed with IFN-gamma and stimulated with LPS in the presence of Am-25-d secreted more IL-1 beta, IL-8, IL-10, IL-12p40, IL-23 and TNF-alpha than THP-1 cells stimulated in the absence of Am-25-d. However, when added to unstimulated cells Am-25-d did not increase secretion of the cytokines examined. Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-kappa B and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d. These findings indicate that Am-25-d isolated from A. millefolium has immunoenhancing properties that may be mediated via the Akt pathway. (C) 2016 Elsevier Ltd. All rights reserved.
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