Article
Cell Biology
Clara Apicella, Camino S. M. Ruano, Sebastien Jacques, Geraldine Gascoin, Celine Mehats, Daniel Vaiman, Francisco Miralles
Summary: Through analyzing the expression of non-coding genes in trophoblast fusion and placental diseases, key roles of miR-193b and UCA1 in cell function and gene expression have been identified. Knocking down UCA1 leads to the dysregulation of important trophoblast physiology genes, while in cells overexpressing STOX1B, the effects of UCA1 down-regulation are alleviated.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Peripheral Vascular Disease
Xi Yuan, Xiyao Liu, Fangyu Zhu, Biao Huang, Li Lin, Jiayu Huang, Li Wen, Mark D. Kilby, Philip N. Baker, Yong Fu, Weiwei Wu, Hongbo Qi, Jing Tang, Chao Tong
Summary: This study investigates the relationship among HtrA4, endoplasmic reticulum stress (ERS), and trophoblast syncytialization in the development of early-onset preeclampsia (EO-PE). The findings suggest that ERS enables syncytialization of placental development by upregulating HtrA4, but excessive HtrA4 expression and preexisting ERS impair syncytialization and cause EO-PE.
JOURNAL OF HYPERTENSION
(2023)
Article
Medicine, Research & Experimental
Tao Xu, Xiangrong Xu, Yuankui Chu, Dan Jiang, Guangxian Xu
Summary: The study investigated the role of lncRNA GAS in macrophage autophagy, showing that GAS5 overexpression can enhance autophagic body formation and promote autophagy by sponging miR-181c-5p and miR-1192 to upregulate ATG5 and ATG12.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Peripheral Vascular Disease
Chuyu Peng, Jianbin Zhu, Hong Guo, Ling Zhao, Feifei Wu, Bo Liu
Summary: Objective: Our study aimed to investigate the role of long noncoding RNA (lncRNA) TLR8-AS1 in regulating preeclampsia. Methods: TLR8-AS1 expression was examined in clinical placental tissues of preeclampsia patients and LPS-induced trophoblast cells. The functional role of TLR8-AS1 was studied by infecting different lentivirus into trophoblast cells. Interactions among TLR8-AS1, STAT1 and TLR8 were also determined. Furthermore, a rat model of preeclampsia was developed for in vivo validation of the in-vitro findings. Results: High expression of TLR8-AS1 was detected in placental tissues of preeclampsia patients and LPS-induced trophoblast cells. Overexpression of TLR8-AS1 inhibited the proliferation, migration and invasion of trophoblast cells through upregulation of TLR8 expression. Mechanistically, TLR8-AS1 recruited STAT1 to bind to the TLR8 promoter region, thereby promoting TLR8 transcription. Overexpression of TLR8-AS1 in vivo aggravated preeclampsia by elevating TLR8 expression. Conclusion: Our study confirmed that TLR8-AS1 aggravates the progression of preeclampsia by increasing the expression of STAT1 and TLR8.
JOURNAL OF HYPERTENSION
(2023)
Article
Biochemistry & Molecular Biology
Natasha de Alwis, Bianca R. Fato, Sally Beard, Natalie K. Binder, Tu'uhevaha J. Kaitu'u-Lino, Kenji Onda, Natalie J. Hannan
Summary: The study demonstrated that both esomeprazole MH and MTH could reduce key factors associated with preeclampsia, but esomeprazole MH was more efficacious in vitro.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Peripheral Vascular Disease
Brooke Armistead, Leena Kadam, Emily Siegwald, Fergus P. McCarthy, John C. Kingdom, Hamid-Reza Kohan-Ghadr, Sascha Drewlo
Summary: Preeclampsia is a severe hypertensive disorder of pregnancy caused by placental villi pathology leading to widespread maternal endothelial dysfunction. Modulating trophoblast differentiation pathway through PPAR gamma-GCM1 axis may reduce excessive sFLT1 production and improve clinical outcomes. Pharmacological manipulation of PPAR gamma activity shows potential to rescue the antiangiogenic state of preeclampsia.
Article
Chemistry, Multidisciplinary
Lijun Huang, Zhaowei Tu, Liudan Wei, Wei Sun, Yifan Wang, Shilei Bi, Fang He, Lili Du, Jingsi Chen, Julia Kzhyshkowska, Haibin Wang, Dunjin Chen, Shuang Zhang
Summary: The interaction between trophoblasts, stroma cells, and immune cells at the maternal-fetal interface is crucial for successful pregnancy outcomes. However, efficient experimental models are lacking to investigate this complex interplay. To address this challenge, a reliable method for generating placenta villi organoids (PVOs) from different pregnancies is developed, enabling the analysis of cellular components and genetic alterations. PVOs derived from patients with preeclampsia exhibit specific pathological features. The development of PVOs can provide insights into placenta development and placenta-related diseases.
Article
Biotechnology & Applied Microbiology
Liu Jinyu, Wang Shuying, Zheng Panchan, Chen Dan, Chen Chao, Yang Xingyu, Cheng Weiwei
Summary: The expression of BST2 and MMP2 is significantly downregulated in preeclampsia. Overexpression of BST2 can upregulate MMP2 expression and enhance the migration and invasion capacity of trophoblast cells. Knockdown of BST2 or MMP2 has opposite effects. These results suggest that the downregulation of BST2 may play a role in the pathogenesis of preeclampsia.
Article
Biochemistry & Molecular Biology
Heejin Lee, Hoin Kang, Chongtae Kim, Ja-Lok Ku, Sukwoo Nam, Eun Kyung Lee
Summary: This study found that long non-coding RNA (lncRNA) GAS5 is downregulated in colon cancer cell lines resistant to 5-fluorouracil (5-FU). GAS5 downregulation decreased the viability of HCT116 cells and the level of the pro-apoptotic protein BAX, while GAS5 overexpression promoted cell death in response to 5-FU. GAS5 promoted cell death by interfering with miR-128-3p-mediated BAX downregulation, suggesting that GAS5 overexpression in chemo-resistant cancer cells may improve the efficacy of anti-cancer drugs.
Article
Biochemistry & Molecular Biology
Heyam Hayder, Guodong Fu, Lubna Nadeem, Jacob A. O'Brien, Stephen J. Lye, Chun Peng
Summary: The overexpression of miR-210-3p reduces trophoblast migration and invasion, affects EVT outgrowth in early pregnancy explants, and decreases the ability of trophoblast to form endothelial-like networks. Furthermore, miR-210-3p downregulates the expression of certain cytokines, potentially influencing EVT invasion and immune cell recruitment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Shiyuan Wu, Lingyan Liu, Ting Tao, Jingyan Xiao, Huizhi Yang, Xiaoshan Yu, Jun Chen, Zhiqin Tan, Peng Wu
Summary: This study reveals the significant role of circPTK2 in preeclampsia (PE) and its mechanism through the circPTK2/miR-619/WNT7B axis. The findings suggest the potential use of circPTK2 in the diagnosis and treatment of PE.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yue Bian, Jiapo Li, Hongfei Shen, Yuanyuan Li, Yue Hou, Ling Huang, Guiyu Song, Chong Qiao
Summary: This study found that the level of N6-methyladenosine (m6A) in placental tissue of early-onset preeclampsia (ePE) patients was reduced. Wilms' tumor 1-associating protein (WTAP), a regulator of m6A, was found to regulate trophoblast cell migration and invasion. The study also revealed the interaction between WTAP and the high mobility group nucleosomal binding domain 3 (HMGN3) gene, and the effect of m6A on the stability of HMGN3 mRNA. WTAP enhanced the stability of HMGN3 mRNA by binding to its m6A site, and HMGN3 was recognized and regulated by insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1). The results suggest that m6A modification plays an important role in the pathogenesis of ePE.
Article
Biochemistry & Molecular Biology
Hamid-Reza Kohan-Ghadr, Brooke Armistead, Mikaela Berg, Sascha Drewlo
Summary: Irisin is a newly discovered exercise-mediated polypeptide hormone. Studies have shown that Irisin protects against cell death by reducing pro-apoptotic signaling cascades, inducing DNA repair pathways, and inhibiting Caspase 3 activity. Additionally, Irisin acts through the Akt signaling pathway to prevent apoptosis, increase levels of anti-apoptotic BCL2, and reduce ROS levels. These findings provide new insights into the role of Irisin in promoting cell survival and preventing apoptosis in the human placenta under pathological conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Agata Sakowicz, Michalina Bralewska, Piotr Kamola, Tadeusz Pietrucha
Summary: In vivo studies on gestational pathology often use small mammals as models due to their similarities to humans in terms of reproductive cycle and placental development. However, there are differences in the process of vessel remodeling and trophoblast invasion between these animals and humans, limiting their use in studying preeclampsia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Zhonglu Ren, Yunfei Gao, Yue Gao, Guanmei Liang, Qian Chen, Sijia Jiang, Xiaoxue Yang, Cuixia Fan, Haizhen Wang, Jing Wang, Yi-Wu Shi, Chaoqun Xiao, Mei Zhong, Xinping Yang
Summary: The study found that early-onset severe preeclampsia and late-onset severe preeclampsia have different molecular mechanisms, with the former associated with metabolism-related pathways and the latter with immune-related pathways. Some key transcription factors may be driving the widespread gene dysregulation in both early-onset and late-onset patients.
Article
Multidisciplinary Sciences
Hafiz Abdullah Shakir, Arfa Anwar, Muhammad Irfan, Muhammad Khan, Shaukat Ali, Javed Iqbal Qazi
IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A-SCIENCE
(2020)
Review
Oncology
Sayyeda Hira Hassan, Sameena Gul, Hafiza Sadaf Zahra, Amara Maryam, Hafiz Abdullah Shakir, Muhammad Khan, Muhammad Irfan
Summary: Cancer is a major cause of death worldwide, despite advancements in treatment methods, natural products like alpha-solanine are being explored as potential alternative therapies. Alpha-solanine, primarily found in potato peel, shows promising anticancer activity and could offer an effective and cost-efficient source for cancer therapy.
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
(2021)
Review
Chemistry, Medicinal
Aisha Ashaq, Muhammad F. Maqbool, Amara Maryam, Muhammad Khan, Hafiz A. Shakir, Muhammad Irfan, Javed I. Qazi, Yongming Li, Tonghui Ma
Summary: HIS, a biologically active natural flavone, exhibits multitargeted anticancer activities by modulating multiple signaling pathways related to cancer initiation and progression. This review provides information on the natural sources, anticancer mechanism, cellular targets, and chemo-sensitizing potential of HIS.
PHYTOTHERAPY RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Muhammad Zubair Saleem, Mohammed Alshwmi, He Zhang, Syed Riaz Ud Din, Muhammad Azhar Nisar, Muhammad Khan, Shahid Alam, Gulzar Alam, Lingling Jin, Tonghui Ma
FRONTIERS IN PHARMACOLOGY
(2020)
Article
Chemistry, Physical
Shabbir Muhammad, Sayyeda Hira Hassan, Abdullah G. Al-Sehemi, Hafiz Abdullah Shakir, Muhammad Khan, Muhammad Irfan, Javed Iqbal
Summary: Ellagic acid and apigenin, compounds from Moringa oliefera, show high binding affinities and dynamic behavior when interacting with crucial proteins of COVID-19. They also exhibit the highest absorption and clearance rates compared to other compounds under study, without any toxicity.
CHEMICAL PHYSICS LETTERS
(2021)
Article
Nanoscience & Nanotechnology
Asia Iqbal, Muhammad Zakir, Muhammad Muddassir Ali, Shagufta Irshad, Arshad Javid, Muhammad Khan, Chaman Ara, Asmatullah
Summary: The study evaluated the effects of Allium cepa-mediated zinc oxide NPs on male reproductive tissues and aberrations in sperm structure of albino mice. The synthesized NPs showed negative effects on sperm parameters and testicular tissues.
APPLIED NANOSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Erum Zafar, Muhammad Faisal Maqbool, Asia Iqbal, Amara Maryam, Hafiz Abdullah Shakir, Muhammad Irfan, Muhammad Khan, Yongming Li, Tonghui Ma
Summary: BZA, a synthetic selective estrogen receptor modulator, has shown promising anticancer activity in various cancers through inhibiting multiple signaling pathways and inducing apoptosis, as well as enhancing the efficacy of chemotherapeutic drugs in clinical studies.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Sameena Gul, Muhammad Faisal Maqbool, Amara Maryam, Muhammad Khan, Hafiz Abdullah Shakir, Muhammad Irfan, Chaman Ara, Yongming Li, Tonghui Ma
Summary: Cancer incidence is increasing rapidly worldwide, posing a significant challenge for treatment. Drug resistance is a major obstacle in successful cancer treatment. Combining vitamin K analogs with other chemotherapy drugs has been shown to be a safe and efficient way to overcome drug resistance and improve the effectiveness of chemotherapy.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Zoology
Farah Deeba, Hafiz Abdullah Shakir, Muhammad Irfan, Muhammad Khan, Javed Iqbal Qazi
Summary: Chitinase is a highly promising natural enzyme with various applications. Through isolation and optimization, the highest chitinase-producing strain was identified as Bacillus subtilis. The use of agro-industrial waste as a medium component not only reduces production costs but also provides a solution to environmental pollution.
PAKISTAN JOURNAL OF ZOOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Sameena Gul, Muhammad Faisal Maqbool, Dongying Zheng, Yongming Li, Muhammad Khan, Tonghui Ma
Summary: The review compiles data on the natural sources of Alpinetin (ALPI) and its anticancer activity in various human cancers, highlighting its potential as a bioactive dietary nutraceutical. It sets the stage for further pre-clinical and clinical trials to develop ALPI as a lead structure for oncological therapy.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Review
Zoology
Sameena Gul, Sayyeda Hira Hassan, Amara Maryam, Hafiz Abdullah Shakir, Muhammad Khan, Muhammad Irfan, Farah Rauf Shakoori, Javed Iqbal Qazi
Summary: This article discusses the potential mechanisms of EGCG in treating cancer. EGCG has been found to enhance apoptosis and inhibit cancer cell growth by affecting signaling molecules and cell cycle regulatory proteins. Clinical studies have shown that EGCG, when used in combination with other common clinical drugs, can improve the efficacy of chemotherapy.
PAKISTAN JOURNAL OF ZOOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Misbah Ghazanfar, Muhammad Irfan, Muhammad Nadeem, Hafiz Abdullah Shakir, Muhammad Khan, Irfan Ahmad, Shagufta Saeed, Yue Chen, Lijing Chen
Summary: The present study focuses on the production of bioethanol from Bombax ceiba seed pods by alkali pretreatment. The use of KOH-steam-treated substrates in simultaneous saccharification and fermentation (SSF) resulted in the highest saccharification and ethanol production. Optimization of fermentation parameters further increased ethanol yield.
FERMENTATION-BASEL
(2022)
Review
Biotechnology & Applied Microbiology
Rida Fatima Ahmed, Muhammad Irfan, Hafiz Abdullah Shakir, Muhammad Khan, Lijing Chen
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
(2020)
Article
Oncology
Muhammad Khan, Amara Maryam, Muhammad Zubair Salaam, Hafiz Abdullah Shakir, Javed Iqbal Qazi, Yongming Li, Tonghui Ma
Article
Zoology
Amreen Zahra, Mushtaq A. Saleem, Hasnain Javed, Muhammad Azmat Ullah Khan
Summary: The study reveals a high co-infection of HCV and HIV among male IDUs in four major cities of Central Punjab, Pakistan, with Lahore showing the highest proportion. There is a strong correlation between CD4 cell count and viral titres of HCV and HIV.
PAKISTAN JOURNAL OF ZOOLOGY
(2022)