Article
Microbiology
Farhad Vesuna, Ivan Akhrymuk, Amy Smith, Paul T. Winnard Jr, Shih-Chao Lin, Lauren Panny, Robert Scharpf, Kylene Kehn-Hall, Venu Raman
Summary: SARS-CoV-2 continues to spread globally, with evolving variants that are more transmissive and show reduced vaccine efficacy. Targeting host proteins such as DDX3 with RK-33 can effectively reduce viral load and downregulate SARS-CoV-2 genes, making it a potential strategy to control infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Hung-Hsi Chen, Hsin- Yu, Rudy Rudy, Sim-Lin Lim, Yi-Fen Chen, Shu-Hsing Wu, Shu-Chun Lin, Muh-Hwa Yang, Woan-Yuh Tarn
Summary: The use of antibody arrays revealed that the RNA helicase DDX3 plays a role in modulating the expression of secreted signaling factors in oral squamous cell carcinoma (OSCC) cells. Furthermore, the interaction between DDX3 and the signal recognition particle (SRP) affects the expression of AREG mRNA, contributing to the progression of tumors.
Article
Biochemistry & Molecular Biology
Olga Sergeeva, Tatiana Abakumova, Ilia Kurochkin, Renata Ialchina, Anna Kosyreva, Tatiana Prikazchikova, Varvara Varlamova, Evgeniya Shcherbinina, Timofei Zatsepin
Summary: The study showed that a strong reduction of DDX3 protein leads to similar changes in vitro and in vivo, including cell cycle deregulation and pathway alterations, while a more moderate downregulation resulted in discordant results between in vitro and in vivo, indicating the significant impact of DDX3 protein levels on cell phenotype.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Mingming Chen, Miwako Asanuma, Mari Takahashi, Yuichi Shichino, Mari Mito, Koichi Fujiwara, Hironori Saito, Stephen N. Floor, Nicholas T. Ingolia, Mikiko Sodeoka, Kosuke Dodo, Takuhiro Ito, Shintaro Iwasaki
Summary: The translation inhibitor Rocaglamide A shows promising antitumor activity by clamping eIF4A and DDX3 onto polypurine RNA for selective translational repression. Analysis of a de novo-assembled transcriptome from the natural source of RocA, plant Aglaia, reveals the amino acid critical for RocA binding. The dominant-negative effect of RocA on eIF4A and DDX3 leads to strong translational repression in cancer cells, indicating its tumor toxicity.
CELL CHEMICAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shringar Rao, Cynthia Lungu, Raquel Crespo, Thijs H. Steijaert, Alicja Gorska, Robert-Jan Palstra, Henrieke A. B. Prins, Wilfred van Ijcken, Yvonne M. Mueller, Jeroen J. A. van Kampen, Annelies Verbon, Peter D. Katsikis, Charles A. B. Boucher, Casper Rokx, Rob A. Gruters, Tokameh Mahmoudi
Summary: This study demonstrates the selective induction of cell death in latent HIV-1-infected cells by DDX3 inhibitors, and provides evidence for the reduction of the inducible HIV-1 reservoir in CD4+ T cells from PLWHIV. The pharmacological targeting of DDX3 shows promise in reversing HIV-1 latency and eliminating the latent reservoir.
NATURE COMMUNICATIONS
(2021)
Article
Fisheries
Wen Shi, Na Wang, Bing Han, Mengmeng Zhang, Yanxue Shi, Dandan Zhao, Yanzi Zhang, Fengxia Zhao, Xueting Guan, Jinshan Huang, Min Liu
Summary: The DDX3 gene in chinook salmon plays a role in enhancing innate immunity and antiviral response by activating the type I IFN signaling pathway and inducing the expression of type I IFN. This study reveals the biological role of fish DDX3 in regulating innate immunity and antiviral effect, providing a scientific basis for understanding the antiviral regulation mechanism of innate immunity in fish. These findings can guide the breeding of disease-resistant salmon and prevent viral diseases.
Review
Biochemistry & Molecular Biology
Steven M. Heaton, Paul R. Gorry, Natalie A. Borg
Summary: Antiretroviral therapy (ART) is effective in reducing HIV-1 infection. However, the emergence of treatment-refractory variants remains a challenge. HIV-1 uses cellular helicases, including DDX3X, to facilitate its replication. Exploring these helicases may reveal new therapeutic targets and help prevent viral escape.
TRENDS IN MICROBIOLOGY
(2023)
Article
Cell Biology
Despoina Sakellariou, Sara Thornby Bak, Esin Isik, Sonia Barroso, Antonio Porro, Andres Aguilera, Jiri Bartek, Pavel Janscak, Javier Pena-Diaz
Summary: The study reveals that the mismatch repair protein MutS beta is enriched at telomeres in ALT cancer cells, preventing the accumulation of G-quadruplex (G4) structures and R-loops, thus maintaining telomere integrity during alternative lengthening of telomeres.
Article
Biochemistry & Molecular Biology
Litong Yao, Qian Hao, Mozhi Wang, Yuhai Chen, Hongyi Cao, Qiang Zhang, Keda Yu, Yizhou Jiang, Zhiming Shao, Xiang Zhou, Yingying Xu
Summary: In this study, researchers identified KLHL29 as a crucial tumor suppressor that regulates chemosensitivity in triple-negative breast cancer (TNBC). Low levels of KLHL29 were associated with unfavorable prognoses. Mechanistically, KLHL29 suppressed TNBC growth by degrading DDX3X and inhibiting cell cycle progression. The combination of DDX3X inhibitor RK33 and platinum-based chemotherapy showed promising results in suppressing TNBC.
Article
Biochemistry & Molecular Biology
Xu-hui Zhu, Bao-fei Sun, Mei Luo, Jia Yu, Yun-dong Zhang, Hou-qiang Xu, Heng Luo
Summary: The G-quadruplex DNA structure in cancer cells requires the involvement of DNA helicase for unwinding, and the helicase can bind to and unwind G4DNA. The binding and unwinding of G4DNA by helicase is influenced by the single-stranded DNA terminals in G4DNA.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Gastroenterology & Hepatology
Amit Kumar Mishra, Musa Hossain, Mohd Umar, Teja Naveen Sata, Ajay K. K. Yadav, Amrendra Kumar Sah, Md Ismail, Baibaswata Nayak, Senthil Kumar Shalimar, Senthil Kumar Venugopal
Summary: This study reveals that DDX3 inhibits autophagy in HBV-infected cells by upregulating miR-34 expression, while HBx protein helps HBV evade DDX3-mediated inhibition by downregulating DDX3 expression.
JOURNAL OF VIRAL HEPATITIS
(2023)
Article
Oncology
Lidia Chellini, Marco Pieraccioli, Claudio Sette, Maria Paola Paronetto
Summary: DHX9 plays an important role in the development of prostate cancer, with up-regulation of DHX9 correlating with advanced stage and poor prognosis. The limited treatment options for patients in the castration-resistant stage make DHX9 a potential new druggable target.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Review
Oncology
Atsushi Tanabe, Takatoshi Nakayama, Jo Kashiyanagi, Hitoshi Yamaga, Yoshihiko Hirohashi, Toshihiko Torigoe, Fukino Satomi, Hiroaki Shima, Hideki Maeda, Goro Kutomi, Ichiro Takemasa, Hiroeki Sahara
Summary: YTHDC2 is an important regulator for breast cancer tumorigenesis and metastasis, and its expression level is significantly correlated with tumor stage. Knockdown of YTHDC2 attenuates sphere-forming and metastatic ability of breast cancer cells, but the mechanism by which YTHDC2 regulates these phenotypes is different between different subtypes of breast cancer.
JOURNAL OF ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Yujie Zhu, Mingchao Zhang, Weiran Wang, Shuang Qu, Minghui Liu, Weiwei Rong, Wenwen Yang, Hongwei Liang, Caihong Zeng, Xiaodong Zhu, Limin Li, Zhihong Liu, Ke Zen
Summary: Reduction of renal tubular cell PNPT1 causes renal tubular translation arrest and atrophy, which leads to tubular injury. Increasing PNPT1 expression can alleviate tubular injury.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Kevin J. Tu, Sanjit K. Roy, Zachery Keepers, Manas R. Gartia, Hem D. Shukla, Nrusingh C. Biswal
Summary: The study investigates the effectiveness and molecular characteristics of the combination therapy of Docetaxel (DXL) and radiation in vitro. The results suggest that DXL sensitizes castration-resistant prostate cancer (CRPC) cells to radiation by downregulating CAV-1.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2023)
