Article
Engineering, Environmental
Weigang Wang, Tong Wang, Qinghua Liu, Han Wang, Hao Xue, Zhuoran Zhang, Yayi Wang
Summary: Using bamboo biochar as a mediator can stimulate the DNRA pathway of anammox bacteria and enhance nitrogen removal efficiency.
Article
Agricultural Engineering
Lili Yang, Wenxuan Li, Hongjuan Zhu, Sanqiang Dong, Hao Mu, Kaiyao Hu, Te Wang, Jie Li
Summary: Sponge iron (SI) has promising potential for nitrogen removal in the Anammox process, as it enhances the expression of specific key genes and improves nitrogen removal efficiency.
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
Chao Wang, Sen Qiao, Zhen Bi, Jiti Zhou
Summary: A novel nitrate reduction pathway by anammox biomass using intracellular carbon sources as electron donors was discovered, with the potential to enhance bacterial viability in different ecosystems and achieve complete nitrogen removal.
ENVIRONMENTAL RESEARCH
(2021)
Article
Environmental Sciences
Feng Sun, Qinghui Deng, Xiaowen Li, Mengjuan Tang, Xufa Ma, Xiuyun Cao, Yiyong Zhou, Chunlei Song
Summary: The study found that high organic carbon content in matrix facilitated dissimilatory nitrate reduction to ammonium (DNRA) over denitrification (DEN), while low organic carbon content promoted DEN. It was also observed that using corn cob as OC source in biofilters was more effective than bagasse due to slow and continuous release of organic carbon. Choosing suitable OC source and regulating its dosage are crucial for enhancing nitrate removal and reducing ammonium accumulation in biofilters.
Article
Agricultural Engineering
Xiaodan Zhao, Jie Jiang, Zhen Zhou, Yue Zheng, Yanjun Shao, Yi Zuo, Yuqing Ren, Ying An
Summary: This study investigated the effects of hydraulic retention time of anaerobic side-stream reactors on nitrogen removal in four anoxic/oxic membrane bioreactors. The results showed that upgrading with anaerobic side-stream reactors and carriers can enhance nitrogen removal efficiency by increasing denitrifying bacteria and secondary substrate release. Additionally, metagenomic analysis revealed higher abundances of genes related to nitrogen consumption processes in the upgraded reactors, confirming their pivotal roles in nitrogen metabolism.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Peike Gao, Yu Gao, Hongbo Wang, Ting Ma, Ji-Dong Gu
Summary: This study identifies the presence of anammox bacteria in oil reservoirs and suggests that they are more easily detected in the downhole of water-injection wells. These bacteria, along with other bacteria, participate in the nitrogen cycle in oil reservoirs and potentially reduce externally injected nitrate through the production of N2O and N2. This research improves our understanding of anammox bacteria detection and highlights their roles in the nitrogen cycle in oil reservoirs.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2023)
Article
Green & Sustainable Science & Technology
Chao Wang, Sen Qiao, Jiti Zhou
Summary: This study found that anaerobic ammonium oxidation (anammox) bacteria can use nitrate (NO3-) as the sole substrate and decompose intracellular carbon source (glycogen) to support nitrogen metabolism. The transcription gene and protein expression analysis also revealed the activation of key functions and metabolism pathways in anammox bacteria. This research provides new insights into the complete nitrogen removal ability and viability of anammox bacteria in different natural ecosystems.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Engineering, Environmental
Qingkun Wang, Matthew James Rogers, Sir Sing Ng, Jianzhong He
Summary: The study sheds light on the significance of anammox activity and its association with sulfide-driven dissimilatory nitrate reduction to ammonium (DNRA) in tropical wetlands. Microbial community analysis revealed a diverse array of anammox bacteria, and correlation analysis suggested potential syntrophic interactions among sulfate-reducing, sulfide-driven DNRA, and anammox bacterial populations.
Article
Environmental Sciences
Xiaolong Yao, Zhonghua Zhao, Jianjun Wang, Qiqi Ding, Minglei Ren, Ismael Aaron Kimirei, Lu Zhang
Summary: The nitrogen transformation in East African lakes is mainly driven by benthic organic matter properties and water column phosphate concentrations, and the sediment nitrogen supply and loss can be improved by considering sediment organic matter properties.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Green & Sustainable Science & Technology
Manping Zhang, Jung-Chen Huang, Shanshan Sun, Muhammad Muneeb Ur Rehman, Shengbing He, Weili Zhou
Summary: The study found that denitrification is the main mechanism of nitrate reduction in constructed wetlands, with the majority of nitrogen gas produced through this process. The rates of three microbial nitrate reduction processes were significantly higher in the PA wetland than in the NP wetland. Additionally, nitrate plays a crucial role in the competition between DNRA and denitrification, while nitrite regulates anammox.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Microbiology
Samantha G. Fortin, Bongkeun Song, Iris C. Anderson
Summary: This study found that denitrification is the primary process for nitrogen removal in the York River Estuary, while DNRA is the main process for nitrogen retention. Denitrification rates are strongly correlated with concentrations of organic matter, nitrate and chlorophyll a, while DNRA rates are best correlated with the relative abundance of specific bacterial families carrying the nrfA gene.
FEMS MICROBIOLOGY ECOLOGY
(2021)
Article
Environmental Sciences
Xiaoling Li, Jianqiang Zhao, Yuhao Zhang, Jiaojie He, Kaili Ma, Chunshuang Liu
Summary: This study found that the proportion of DNRA increases in high-electron systems, particularly in organic-rich or sulfide-rich systems, indicating that excess electron donors favor DNRA. Additionally, a higher reductive environment also facilitates DNRA. 16S rRNA gene sequencing analysis revealed different participants involved in the DNRA process in organic-rich and sulfide-rich systems.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Agricultural Engineering
Hao Sheng, Rui Weng, Yan He, Zheng Wei, Yanmei Yang, Jinghan Chen, Minsheng Huang, Gongming Zhou
Summary: This study identified a nitrate-calcium-based pathway that accelerated the start-up of anammox. Calcium nitrate induced partial denitrification to provide nitrite for anammox, while also increasing the contribution of dissimilatory nitrate reduction to ammonium (DNRA). This mutualistic symbiosis among denitrification, DNRA, and anammox in the calcium nitrate-added systems explained the accelerated start-up of anammox.
BIORESOURCE TECHNOLOGY
(2021)
Article
Environmental Sciences
Mingcong Li, Guangshan Wei, Jiai Liu, Xiaoyun Wang, Lijun Hou, Zheng Gao
Summary: This study investigated the effects of nitrate concentration on nitrate reduction rates and microbial communities in wetland sediments from the Yellow River estuary. Results showed that increasing nitrate concentration led to higher denitrification rates, lower anaerobic ammonium oxidation rates, and changes in bacterial community compositions. Nitrate concentration can be divided into high and low groups, impacting microbial relationships within estuarine environments.
ESTUARIES AND COASTS
(2022)
Article
Engineering, Environmental
Linqin Tang, Chengyuan Su, Yu Chen, Yunchuan Xian, Xinyue Hui, Ziyu Ye, Menglin Chen, Fenghua Zhu, He Zhong
Summary: Research has found that PBS and PVC microplastics reduce the nitrite removal efficiency of anammox sludge, while PBS MPs have a positive effect on the polysaccharide content in the sludge. In addition, non-biodegradable PVC MPs are more harmful to anammox sludge and warrant attention.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Microbiology
Xuejiao Qiao, Liyu Zhang, Zhiguang Qiu, Li Wang, Yang Wu, Chunfang Deng, Jia Su, Xue Zhang, Yuexing Wang, Bing Li, Lijie Zhou, Anthony Y. W. Ma, Wei-Qin Zhuang, Ke Yu
Summary: This study investigated the microbial shift and functional interactions of AnAOB, DN, and DNRA bacteria during long-term nitrite inhibition and self-recovery in a laboratory-scale anammox bioreactor. The results showed that DN and DNRA bacteria could stabilize the nitrite concentration and contribute to system recovery under high nitrite conditions. These findings provide potential engineering solutions for maintaining a robust and efficient industrial-scale anammox process.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agricultural Engineering
Lijie Zhou, Pingxiang Ou, Zhiyuan Shao, Yichang Shen, Jiahao Lu, Wei-Qin Zhuang
Summary: This study investigated the dissimilatory sulfate reduction (DSR) process in the cake layer of a full-scale anaerobic dynamic membrane bioreactor for treating hotel laundry wastewater. The results showed that the development of the cake layer was positively correlated with the change in sulfate concentration and negatively correlated with the change in sulfide concentration. DSR was identified as the dominant sulfate reduction process in the cake layer. Metagenomic analysis revealed that the sulfate reduction in the cake layer was mainly carried out by collaborations between bacteria with incomplete DSR pathways.
BIORESOURCE TECHNOLOGY
(2022)
Article
Environmental Sciences
Lijie Zhou, Bikai Zhao, Yuanzhong Lin, Zhiyuan Shao, Rongjie Zeng, Yichang Shen, Wenyu Zhang, Yixin Jian, Wei-Qin Zhuang
Summary: This study investigated the DNRA and denitrification activities in the dynamic cake layer of a full-scale AnDMBR for treating hotel laundry wastewater. It was found that DNRA activity and accumulation of bacteria with a complete DNRA pathway were positively correlated with the development of the dynamic cake layer. Metagenomic analysis revealed that approximately 18% of the bacterial community in the dynamic cake layer possessed a complete DNRA pathway. Additionally, COD loading rate could be used to control DNRA and denitrification activities during the formation of the dynamic cake layer.
Article
Engineering, Environmental
Shan Yi, Katie C. Harding-Marjanovic, Erika F. Houtz, Edmund Antell, Christopher Olivares, Rita V. Nichiporuk, Anthony T. Iavarone, Wei-Qin Zhuang, Jennifer A. Field, David L. Sedlak, Lisa Alvarez-Cohen
Summary: This study investigated the biotransformation of PFAS under nitrate-reducing conditions and found that the rates were slower compared to aerobic conditions but faster than sulfate-reducing conditions. The formation of some PFAS products was observed, and evidence for the formation of volatile PFAS was obtained.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Environmental Sciences
Lijie Zhou, Yongzhou Lai, Rongjie Zeng, Bikai Zhao, Yixin Jian, Pingxiang Ou, Wenyu Zhang, How Yong Ng, Wei-Qin Zhuang
Summary: Microbial carbon fixation pathways in membrane cake layer formation processes are not fully understood. This study used meta-omics methods to investigate carbon fixation pathways in a full-scale anoxic-oxic biofilm-MBR system treating textile wastewater, identifying potential pathways like the Calvin-Benson-Bassham cycle and the 3-hydroxypropionate bicycle. Aeration was found to inhibit carbon fixation and mitigate membrane cake layer development, although it also increased the abundance of the 3-hydroxypropionate bicycle pathway in aerobic conditions.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Teresa Scott, Colin Whittaker, Wei-Qin Zhuang, Bruce Melville
Summary: This study examines the design and operation issues of pressure sewer networks in New Zealand. The findings suggest that the current design assumptions are too high and may lead to operational problems. Additionally, the study reveals the impact of COVID-19 lockdown on wastewater inflow volumes.
JOURNAL OF ENVIRONMENTAL ENGINEERING
(2023)
Article
Engineering, Environmental
Zhiyuan Shao, Yichang Shen, Zhihang Zeng, Yixin Jian, Marc Russenberger, Lijie Zhou, Wei-Qin Zhuang
Summary: This study investigates the crash of denitrification during tofu processing wastewater treatment in an anaerobic biofilm reactor (ABR), and identifies dissimilatory nitrate reduction to ammonium (DNRA) as the cause. Metagenomic analysis and enzyme activity measurements reveal variations in microbial communities and functional genes related to denitrification and DNRA. The crash occurs due to the high COD:TN ratio in the wastewater, inhibiting denitrification and promoting DNRA. Denitrifiers are replaced by DNRA bacteria as the dominant nitrate-reducing microorganisms. The study highlights the importance of COD:TN ratio control for efficient nitrogen removal in ABRs.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Article
Engineering, Environmental
Lijie Zhou, Bikai Zhao, Wei-Qin Zhuang
Summary: The double-edged sword effects of Dissimilatory nitrate reduction to ammonium (DNRA) bacteria on anammox bacteria were investigated. It was found that the overgrowth of DNRA bacteria had a negative impact on the nitrogen removal efficiency of anammox bacteria.
Article
Biotechnology & Applied Microbiology
Li Wang, Chunang Lian, Wenjie Wan, Zhiguang Qiu, Xuesong Luo, Qiaoyun Huang, Ye Deng, Tong Zhang, Ke Yu
Summary: Climate change and human activities have led to the gradual salinization of inland waters worldwide, impacting prokaryotic plankton communities and biogeochemical cycles. This study analyzed the prokaryotic plankton communities of 11 lakes in northwest China and found that salinity was the most important driver of beta-diversity. Under low salinity, prokaryotic plankton employed diverse halotolerant strategies, while under high salinity, only organisms with energetically favorable halotolerant strategies survived. High salinity decreased taxonomic diversity and impaired functional diversity related to substance metabolism. The study also revealed a precarious microbial network with increasing salinity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Engineering, Environmental
Lijie Zhou, Yongzhou Lai, Zhiyuan Shao, Yixin Jian, Wei-Qin Zhuang
Summary: Keystone bacteria associated with thiosulfate-driven autotrophic denitrification were identified in this study. Five anoxic bioreactors with varying S/N ratios were fed with synthetic wastewater containing nitrate. The dominant phyla in the bioreactors were Proteobacteria, Bacteriodetes, and Firmicutes, while Thiobacillus, Pseudomonas, Arenimonas, and Thauera were the predominant genera. Thiobacillus and Thauera were found to have the complete nitrate reduction pathway and were essential for thiosulfate oxidation. However, their collaboration might have resulted in extracellular electron transportation and low electron utilization in the autotrophic denitrification process.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Environmental
Yi-Lu Sun, Zhuo-Ran Li, Xue-Ning Zhang, Heng Dong, Zhi-Min Qian, Shan Yi, Wei-Qin Zhuang, Hao-Yi Cheng, Ai-Jie Wang
Summary: Elemental sulfur autotrophic denitrification (S0AD) is a promising method for wastewater treatment with lower costs, zero carbon dioxide emission, and minimum sludge production. This study demonstrated the effectiveness of a pilot-scale S0AD packed-bed in removing nitrate to below the national standard, with temperature and bacteria species playing important roles. The findings provide guidance for the design and operation of S0AD packed beds in practical engineering.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Environmental Sciences
Yichang Shen, Zhihang Zeng, Xi Yue, Haixiang Li, Hukerenui Bonnet, Lijie Zhou, Wei-Qin Zhuang
Summary: The study aimed to investigate the impact of perfluorooctanoic acid (PFOA) on the biological performance of a hydrogen-driven bioreactor for nitrate and arsenate removal. The results showed that PFOA shock inhibited and decreased the maximum hydrogen-driven arsenate removal rate, leading to shifts in the bacterial community population. Therefore, the presence of PFOA has significant implications for the performance of bioreactors in wastewater treatment processes.
ENVIRONMENTAL POLLUTION
(2023)
Article
Environmental Sciences
Lijie Zhou, Nan Dong, Manki Fu, Xi Yue, Yixin Jian, Haixiang Li, Marc Russenberger, Wei-Qin Zhuang
Summary: This study investigated the microbial population and functional genes of dissimilatory sulfate reduction (DSR) in an anaerobic biofilm reactor treating tofu processing wastewater. The results showed that 79.6-85.1% of sulfate was transformed into sulfide, independent of dissolved oxygen supplementation. Metagenomic analysis revealed 21 metagenome-assembled genomes (MAGs) containing DSR enzymes. The dominant DSR species in the biofilm community were Comamonadaceae, Thiobacillus, Nitrosomonadales, Desulfatirhabdium butyrativorans, and Desulfomonile tiedjei. Dissolved oxygen supplementation inhibited DSR and reduced sulfide production.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Materials Science, Multidisciplinary
Yuanzhe Li, Cong Fang, Wei-Qin Zhuang, Haojing Wang, Xueli Wang
Summary: Polyurea coatings, when modified with Ce/La-doped TiO2, exhibit increased mechanical strength, chemical durability, and reduced microbial attachment. The coatings also show improved photocatalytic response to UV light. They are suitable for emergency leak sealing and can prolong maintenance service intervals.
NPJ MATERIALS DEGRADATION
(2022)
Article
Engineering, Environmental
Navid Taghavi, Wei-Qin Zhuang, Saeid Baroutian
Summary: The study showed that a microbial consortium, with the presence of biosurfactant, effectively biodegraded mixed plastics under UV-pretreated and un-pretreated conditions. PS samples demonstrated the highest biodegradation efficiency and physical weight loss, while PE and PET showed higher efficiency only under UV-pretreated and bio-treated conditions. The utilization of biosurfactant negatively affected the biodegradation and wettability of PE and PET.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)