Article
Multidisciplinary Sciences
Remi Le Guern, Teddy Grandjean, Sarah Stabler, Marvin Bauduin, Philippe Gosset, Eric Kipnis, Rodrigue Dessein
Summary: Gut microbiome dysbiosis caused by carbapenemase-producing Enterobacterales (CPE) colonisation is associated with worsened outcomes of Pseudomonas aeruginosa lung infection in mice. The dysbiosis is characterised by decreased alveolar macrophages and conventional dendritic cells, as well as a decrease in the Muribaculaceae family and lower levels of short-chain fatty acids. Faecal microbiota transplantation restores immune responses and lung infection outcomes, indicating the involvement of CPE-induced gut dysbiosis in altering the gut-lung immune axis.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Weijun Xiu, Lili Ren, Huayu Xiao, Yue Zhang, Dou Wang, Kaili Yang, Siyu Wang, Lihui Yuwen, Xiao Li, Heng Dong, Qiang Li, Yongbin Mou, Yuqian Zhang, Zhaowei Yin, Bin Liang, Yu Gao, Lianhui Wang
Summary: An ultrasound-responsive catalytic micro-bubble is developed to combat chronic lung infections caused by Pseudomonas aeruginosa biofilms. These microbubbles physically disrupt the biofilm structure and enhance the penetration of nanoparticles and antibiotics. The nanoparticles chemically degrade the biofilm matrix and kill bacteria, while also activating the immune response. This strategy efficiently treats chronic lung infections by combining physical/chemical/antibiotic biofilm elimination and immune activation.
Article
Respiratory System
Xiaofeng Wu, Feilong Jie, Pingchao Li, Chao Zhuo, Weiqi Pan, Nanshan Zhong, Chufang Li, Ling Chen
Summary: Influenza virus infection complicated by secondary bacterial pneumonia, particularly Pseudomonas aeruginosa, contributes to increased morbidity and mortality. Mice experimentally infected with PR8 influenza virus and later with P. aeruginosa showed reduced body weight, survival rate, and pathological changes in lung tissue. However, immunization with inactivated P. aeruginosa cells or recombinant PcrV protein protected mice from secondary P. aeruginosa infection in the presence of influenza virus infection. Therefore, developing an effective P. aeruginosa vaccine might be a promising strategy to reduce the threat of secondary P. aeruginosa infection in influenza patients.
JOURNAL OF THORACIC DISEASE
(2023)
Article
Microbiology
Danya A. Dean, London Klechka, Ekram Hossain, Adwaita R. Parab, Krystin Eaton, Myron Hinsdale, Laura-Isobel McCall
Summary: The study demonstrates the importance of the influenza virus as a major health concern, and how spatial metabolomics can help understand changes in the lung and serum metabolome of mice infected with influenza A virus compared to uninfected controls.
Article
Infectious Diseases
M. A. Martinez-Garcia, G. Oscullo, T. Posadas, E. Zaldivar, C. Villa, Y. Dobarganes, R. Giron, C. Olveira, L. Maiz, M. Garcia-Clemente, O. Sibila, R. Golpe, J. Rodriguez, E. Barreiro, J. L. Rodriguez, L. Feced-Olmos, C. Prados, A. Muriel, D. de la Rosa
Summary: This study aimed to analyze lung function decline in bronchiectasis patients. Results showed that FEV1 decline rate was associated with factors such as age, chronic bronchial infection by P. aeruginosa, number of previous severe exacerbations, and baseline FEV1 value.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Chemistry, Multidisciplinary
Pengyu Li, Jieyi Pan, Yating Dong, Yingying Sun, Yalong Wang, Kang Liao, Yili Chen, Xin Deng, Shihui Yu, Haiyan Hu
Summary: Chronic pulmonary infection caused by Pseudomonas aeruginosa is a serious public health problem with high mortality rates. In this study, infection-microenvironment responsive nanoparticles were developed to eradicate biofilms and inhibit virulence. These nanoparticles showed promising results in treating chronic pulmonary infections.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Biochemistry & Molecular Biology
Ke-Qin Gong, Carmen Mikacenic, Matthew E. Long, Charles W. Frevert, Timothy P. Birkland, Jean Charron, Sina A. Gharib, Anne M. Manicone
Summary: Inhibition of MAP2K2 can improve acute lung injury (ALI) and deficiency of MAP2K2 can accelerate the resolution of ALI. Genetic variants in MAP2K2 are associated with mortality from acute respiratory distress syndrome (ARDS). MAP2K2 plays an important role in promoting and sustaining proinflammatory pathway activation in ALI.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Ghadah A. AlShaikh-Mubarak, Essam Kotb, Amira H. Alabdalall, Munirah F. Aldayel
Summary: Ninety-one elastase-producing bacterial isolates were recovered from different localities of the Eastern Province of Saudi Arabia. Elastase from the best isolate Priestia megaterium gasm32, from luncheon samples was purified to electrophoretic homogeneity using DEAE-Sepharose CL-6B and Sephadex G-100 chromatographic techniques. The purified elastase showed high antibacterial activity and potential for treating damaged skin fibers with the inhibition of contaminating bacteria.
Article
Multidisciplinary Sciences
Claudie Lamoureux, Charles-Antoine Guilloux, Clemence Beauruelle, Stephanie Gouriou, Sophie Ramel, Anne Dirou, Jean Le Bihan, Krista Revert, Thomas Ropars, Rosyne Lagrafeuille, Sophie Vallet, Rozenn Le Berre, Emmanuel Nowak, Genevieve Hery-Arnaud
Summary: This study described the diversity of anaerobic bacteria in the CF airway microbiota, identifying 31 anaerobic genera and 69 species. Better lung function was significantly associated with higher quantification of Veillonella, raising questions about the potential impact of anaerobes on lung function.
SCIENTIFIC REPORTS
(2021)
Article
Respiratory System
Lucile Regard, Clemence Martin, Jean-Luc Teillaud, Helene Lafoeste, Hugues Vicaire, Maha Zohra Ladjemi, Emilie Ollame-Omvane, Sophie Siberil, Pierre-Regis Burgel
Summary: Depletion of B cells and/or T cells led to disorganisation or abolishment of tertiary lymphoid structures (TLS) in mice persistently infected with S. aureus, showing no impact on infection control.
EUROPEAN RESPIRATORY JOURNAL
(2021)
Article
Multidisciplinary Sciences
Mahere Momenijavid, Himen Salimizand, Aazam Korani, Omid Dianat, Bijan Nouri, Rashid Ramazanzadeh, Amjad Ahmadi, Jino Rostamipour, Mohammad Rastegar Khosravi
Summary: This study evaluated the effects of calcium hydroxide on the morphology and physicochemical properties of Enterococcus faecalis biofilm. It was found that calcium hydroxide could contribute to a denser biofilm structure with more cavities and a granular surface, resulting in increased biomass, thickness, colony size, and volume of the biofilm.
SCIENTIFIC REPORTS
(2022)
Review
Biotechnology & Applied Microbiology
Yu-Ming Cai, Ying-Dan Zhang, Liang Yang
Summary: Nitric oxide (NO) plays a crucial role in controlling microbial infections, particularly in chronic lung infections. While endogenously generated NO helps eradicate invading pathogens in healthy individuals, this pathway is often compromised in patients with chronic lung infections. Exogenous NO supplementation may improve therapeutic outcomes in these cases.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Stephanie van de Wall, Vladimer P. Badovinac, John T. Harty
Summary: This review discusses lung-resident CD8(+) T cells (Trm) generated upon IAV infection, compared to Trm cells in other tissues, addressing major knowledge gaps in the generation and maintenance of IAV-specific lung Trm. The mechanisms contributing to the decline in lung Trm cell numbers are also discussed, highlighting the importance of understanding effector function versus immunopathology for future IAV vaccine design.
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Li Li, Zuxian Xu, Ruipin Cao, Jiaxin Li, Chang-Jer Wu, Yinglu Wang, Hu Zhu
Summary: We investigated the influence of hydroxyl groups on the anti-quorum-sensing (anti-QS) and anti-biofilm activity of cyclic dipeptides against Pseudomonas aeruginosa PAO1. The presence of hydroxyl groups improved the inhibition of virulence factors and cytotoxicity, but reduced the ability to inhibit biofilm formation. These cyclic dipeptides interacted with the QS-related protein LasR, and the introduction of hydroxyl groups enhanced their self-assembly ability.
Editorial Material
Genetics & Heredity
Tatyana L. Povolotsky, Alona Keren-Paz, Ilana Kolodkin-Gal
Summary: For bacteria, the transition from unicellular entities to multicellular biofilm communities generates distinct metabolic microenvironments. Dynamic and programmed metabolic responses allow the biofilms to react to local changes in nutrient levels and contribute to phenotypic antibiotic resistance of the community. Novel therapeutic approaches targeting biofilms may stem from these metabolic adaptations.
TRENDS IN GENETICS
(2021)
Article
Multidisciplinary Sciences
Juliette Simonin, Emmanuelle Bille, Gilles Crambert, Sabrina Noel, Elise Dreano, Aurelie Edwards, Aurelie Hatton, Iwona Pranke, Berengere Villeret, Charles-Henry Cattart, Jean-Patrick Vrel, Valerie Urbach, Nesrine Baatallah, Alexandre Hinzpeter, Anita Golec, Lhousseine Touqui, Xavier Nassif, Luis J. Galietta, Gabrielle Planelles, Jean-Michel Sallenave, Aleksander Edelman, Isabelle Sermet-Gaudelus
SCIENTIFIC REPORTS
(2019)
Article
Engineering, Biomedical
Daniel Sanchez-Guzman, Pierre Le Guen, Berengere Villeret, Nuria Sola, Remi Le Borgne, Alice Guyard, Alix Kemmel, Bruno Crestani, Jean-Michel Sallenave, Ignacio Garcia-Verdugo
Article
Multidisciplinary Sciences
Juliette Simonin, Emmanuelle Bille, Gilles Crambert, Sabrina Noel, Elise Dreano, Aurelie Edwards, Aurelie Hatton, Iwona Pranke, Berengere Villeret, Charles-Henry Cottart, Jean-Patrick Vrel, Valerie Urbach, Nesrine Baatallah, Alexandre Hinzpeter, Anita Golec, Lhousseine Touqui, Xavier Nassif, Luis J. V. Galietta, Gabrielle Planelles, Jean-Michel Sallenave, Aleksander Edelman, Isabelle Sermet-Gaudelus
SCIENTIFIC REPORTS
(2019)
Review
Immunology
Jean-Michel Sallenave, Loic Guillot
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Saade Kheir, Berengere Villeret, Ignacio Garcia-Verdugo, Jean-Michel Sallenave
Summary: This study evaluated the effectiveness of direct pulmonary transplantation of gene-modified macrophages in a mouse model of P.a infection. The genetically modified macrophages provided protection against P.a infection and enhanced epithelial cell proliferation and tissue repair in the lungs.
Article
Biochemistry & Molecular Biology
Berengere Villeret, Reem Ghinnagow, Saade Kheir, Maelys Born-Bony, Jay K. Kolls, Ignacio Garcia-Verdugo, Jean-Michel Sallenave
Summary: This study reveals the importance of lymphocytes in fighting against Pseudomonas aeruginosa infection. The bacterium down-regulates IL-23 and IL-22 protein accumulation in the lungs, but up-regulates their RNA production. Adenovirus-mediated over-expression of IL-1, IL-23, and IL-7 can rescue mice from Pseudomonas aeruginosa-induced lethality, suggesting its potential value in locally immunosuppressed individuals like cystic fibrosis patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lisa Prince, Lydie Martin-Faivre, Berengere Villeret, Daniel Sanchez-Guzman, Pierre Le Guen, Jean-Michel Sallenave, Ignacio Garcia-Verdugo
Summary: Eosinophils play a crucial role in inducing a complete vaccine response, promoting antibody secretion in the early stage of the immune response. They migrate to the lungs and secrete cytokines to activate B cells, promoting antibody production. However, the absence of eosinophils does not impair late immune responses due to a compensating mechanism involving the Th17 pathway. This study reveals a novel role for eosinophils in lung mucosal vaccination, accelerating early immune responses while regulating IgA production in the late stages.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Article
Oncology
Irati Garmendia, Aditi Varthaman, Solenne Marmier, Mahmud Angrini, Ingrid Matchoua, Aurelie Darbois-Delahousse, Nathalie Josseaume, Pierre-Emmanuel Foy, Lubka T. Roumenina, Naira Naouar, Maxime Meylan, Sophie Siberil, Jules Russick, Pierre-Emmanuel Joubert, Karen Leroy, Diane Damotte, Audrey Mansuet-Lupo, Marie Wislez, Marco Alifano, Laurie Menger, Ignacio Garcia-Verdugo, Jean-Michel Sallenave, Olivier Lantz, Florent Petitprez, Isabelle Cremer
Summary: We found that acute influenza A virus infection has a significant impact on the tumor microenvironment and clinical outcomes of patients with non-small cell lung cancer. Influenza virus can infect both tumor and immune cells, resulting in long-term protumoral effects. Infection with influenza virus alters the transcriptional profile of the tumor microenvironment and is associated with poor survival in patients with lung adenocarcinoma.
CANCER IMMUNOLOGY RESEARCH
(2023)
Meeting Abstract
Pediatrics
S. Kheir, B. Villeret, I Garcia-Verdugo, J. Sallenave
PEDIATRIC PULMONOLOGY
(2020)
Meeting Abstract
Pediatrics
J. Sallenave, B. Villeret, B. Solhonne, M. Straube, A. Cazes, I Garcia-Verdugo
PEDIATRIC PULMONOLOGY
(2019)