Article
Biotechnology & Applied Microbiology
Yaqi Wang, Yufei Guo, Wei Cao, Hao Liu
Summary: This study optimized the production of itaconic acid (IA) using A. niger as the chassis. By combining the IA biosynthesis pathways from A. terreus and Ustilago maydis, higher IA titers were achieved. Additionally, increasing the copy number of cadA, blocking IA degradation by deleting ictA, and enhancing the supply of IA precursor by overexpressing acoA all showed positive effects on IA accumulation.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Xianli Xue, Futi Bi, Boya Liu, Jie Li, Lan Zhang, Jian Zhang, Qiang Gao, Depei Wang
Summary: Overexpression of HGT1 improved citric acid production and reduced residual sugar content in the industrial strain A. niger CGMCC 10142. The best-performing strain A. niger 20-15 showed a significant increase in citric acid production with lower sugar content, indicating the potential of HGT1 in enhancing citric acid fermentation.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Hatice Taspinar Demir, Emine Bezirci, Johanna Becker, Hamed Hosseinpour Tehrani, Emrah Nikerel, Nick Wierck, Mustafa Turker
Summary: Metabolically engineered Ustilago maydis strain successfully produced high concentration itaconate in a 16 L fermentation experiment, with gradually decreasing itaconic acid levels and nearly constant product yield observed with successive feedings. Additionally, the decrease in pH was found to affect biomass composition and itaconic acid production.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Nisa Saelee
Summary: Using squeezed sap from old oil palm trunks as a substrate, the LA yield and productivity can be improved by employing a modified constant feed fermentation mode.
FERMENTATION-BASEL
(2022)
Review
Biotechnology & Applied Microbiology
Renwei Zhang, Huan Liu, Yuchen Ning, Yue Yu, Li Deng, Fang Wang
Summary: Itaconic acid (ITA) is a key platform chemical, and its global market is growing due to the demand for bio-based unsaturated polyester resin and its non-toxic properties. However, the current bio-production of ITA faces challenges such as low efficiency, expensive manufacturing costs, and limited availability of inexpensive raw materials. This review highlights recent developments in metabolic engineering for the production of ITA, including pathway identification, engineering of microorganisms, and the design of new strategies for cost-effective and sustainable production.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Y. Aso, Y. Nomura, M. Sano, R. Sato, T. Tanaka, H. Ohara, K. Matsumoto, K. Wada
Summary: The study aimed to enhance the production of alkylitaconic acids by Aspergillus niger S17-5 through the addition of caprylic acid as a precursor. Results showed that addition of caprylic acid improved the production efficiency of 9-HHIA and 10-HHIA, especially when added in the mid-growth phase.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Food Science & Technology
Hao Chen, Jinjing Wang, Qi Li, Xin Xu, Chengtuo Niu, Feiyun Zheng, Chunfeng Liu
Summary: This study developed a mutant yeast strain with high RNA yield and optimized the fermentation process to increase RNA production. Genome sequencing revealed the metabolic mechanisms underlying high RNA production. This work has the potential to reduce the cost of RNA production and shorten the fermentation cycle.
Article
Biotechnology & Applied Microbiology
Paola Monteiro de Oliveira, Larissa Provasi Santos, Luciana Fontes Coelho, Paulo Marcelo Avila Neto, Daiane Cristina Sass, Jonas Contiero
Summary: This study focuses on obtaining L(+)-lactic acid from sucrose using Lactobacillus casei Ke11 strain through different feeding strategies, which increases lactic acid production and productivity, while avoiding cellular stress caused by high osmotic pressure. The purification of the acid using charcoal and celite, followed by a cation exchange column, proved to be highly efficient, allowing for a high yield of lactic acid and removal of sugars and proteins. This study shows potential for large-scale fermentation of L. casei Ke11 for production of l-lactic acid.
FERMENTATION-BASEL
(2021)
Article
Biotechnology & Applied Microbiology
Xianzun Xiao, Liming Ouyang, Jie Qi, Ju Chu
Summary: Aspergillus niger lacks basic genetic elements for dynamic control, with the identification of the first hypoxia-inducible promoters providing new insights into the organism's adaptability to low oxygen conditions. Promoter truncation analysis revealed core regions determining hypoxia response.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Engineering, Chemical
Tomasa Quiterio-Gutierrez, Susana Gonzalez-Morales, Jose Antonio Gonzalez-Fuentes, Adalberto Benavides-Mendoza, Fabian Fernandez-Luqueno, Julia Medrano-Macias, Armando Robledo-Olivo
Summary: The objective of this research was to optimize a culture medium to induce the production of kojic acid by the Aspergillus niger M4 strain in a liquid fermentation process. The culture medium conditions influenced the formation and speed of biomass and the synthesis and yield of kojic acid. The optimum production points were from 72 h and 96 h with 0.552 g/L and 0.510 g/L of kojic acid using 2.5 g/L of yeast extract and with a pH of 5.5.
Article
Biotechnology & Applied Microbiology
Eva Hulber-Beyer, Katalin Belafi-Bako, Tamas Rozsenberszki, Peter Komaromy, Nandor Nemestothy
Summary: The present study evaluates the applicability of semi-continuous fermentation technique for itaconic acid production and investigates the effect of glucose concentration on acid production. The results show that maintaining a constant high glucose concentration can enhance biomass formation and itaconic acid yield.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Thermodynamics
Xianchun Jin, Jiangshan Ma, Jianing Song, Gao-Qiang Liu
Summary: The study demonstrated that high ethanol concentrations can be achieved through fed-batch semi-simultaneous saccharification and fermentation under high biomass loads, with substrate feeding mode showing the best performance among different feeding modes.
Article
Chemistry, Multidisciplinary
Tao Wang, Jiebing You, Xiugang Gong, Shanliang Yang, Lei Wang, Zheng Chang
Summary: In this study, a probabilistic Bayesian deep learning approach is proposed for accurate and robust prediction of product formation in the microbial fermentation process. The Bayesian optimized deep neural network is used as the basic model and the weights of the models are computed recursively based on Bayesian posterior probabilities. The proposed approach shows promising results in capturing the dynamics of fermentation batches and is suitable for online process monitoring.
Article
Agricultural Engineering
Xiaoyu Ma, Ming Gao, Nuohan Wang, Shuo Liu, Qunhui Wang, Xiaohong Sun
Summary: The feasibility of co-fermentation of food waste and spent mushroom substance for lactic acid with Aspergillus niger cellulase replacing commercial cellulase was confirmed in this study, showing positive synergistic effects, and a higher sugar release with Aspergillus niger cellulase.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Chemical
Tamas Rozsenberszki, Peter Komaromy, Eva Hulber-Beyer, Peter Bakonyi, Nandor Nemestothy, Katalin Belafi-Bako
Summary: This study assessed the feasibility of IA separation from fermentation broths produced by Aspergillus terreus using commercialized electrodialysis unit with mono-and bipolar membranes. Experimental results showed that higher initial pH resulted in faster IA separation but decreased current efficiency, with by-products affecting separation efficiency.
CHEMICAL ENGINEERING RESEARCH & DESIGN
(2021)