期刊
NANOMATERIALS
卷 10, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/nano10010092
关键词
colorimetric bacteria detection; chitosan-coated magnetic nanoparticles; peroxidase-like activity; Escherichia coli; Staphylococcus aureus
类别
资金
- Center for Research and Development of the Police Science and Technology - Korea National Police Agency (KNPA) [PA-K000001-2019-401]
- National Research Foundation of Korea (NRF) - Korea government (Ministry of Science and ICT) [NRF-2019R1A2C1087459]
- Korea Agency for Infrastructure Technology Advancement (KAIA) [PA-K000001-2019-401] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
An effective novel strategy to detect bacteria is promising because it may improve human health by allowing early diagnosis and timely treatment of bacterial infections. Here, we report a simple, reliable, and economical colorimetric assay using the peroxidase-like activity of chitosan-coated iron oxide magnetic nanoparticles (CS-MNPs). When CS-MNPs are incubated with a sample containing bacterial cells such as the gram-negative Escherichia coli or the gram-positive Staphylococcus aureus, the negatively-charged bacterial membrane interacts with positively-charged chitosan on the surface of CS-MNPs, thus resulting in significant reduction of their peroxidase-like activity presumably by a hindrance in the accessibility of the negatively charged substrate, 2-2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) to the positively-charged CS-MNPs. This simple colorimetric strategy allowed the rapid detection of bacterial cells down to 10(4) CFU mL(-1) by the naked eye and 10(2) CFU mL(-1) by spectrophotometry within 10 min. Based on the results, we anticipate that the CS-MNPs-based assay has great potential for the on-site diagnosis of bacterial infections in facility-limited or point-of-care testing (POCT) environments.
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