Article
Oncology
Serena Stamatakos, Giovanni Luca Beretta, Elisabetta Vergani, Matteo Dugo, Cristina Corno, Elisabetta Corna, Stella Tinelli, Simona Frigerio, Emilio Ciusani, Monica Rodolfo, Paola Perego, Laura Gatti
Summary: The study found that targeting FASN in melanoma cells resistant to BRAF inhibitors increased sensitivity to the drug, while also activating compensatory pathways. Metabolic changes promoting cell survival are involved in melanoma progression and drug resistance. FASN plays a role in BRAF-mutant melanoma progression, offering new therapeutic opportunities for treatment.
Article
Chemistry, Analytical
Zixuan Zhou, Yan Chen, Xiang Qian
Summary: In this paper, a method for rapid and efficient isolation of exosomes by targeting exosome-carrying CD63 and PTK 7 in a microfluidics system using modified microchannels and aptamers is proposed.
Article
Biochemistry & Molecular Biology
Anne Uebel, Stefanie Kewitz-Hempel, Edith Willscher, Kathleen Gebhardt, Cord Sunderkoetter, Dennis Gerloff
Summary: Activating BRAF mutations are present in 50-60% of malignant melanomas. Inhibiting EZH2 or its downstream target PLK1, in combination with BRAF inhibitors, may provide potential novel therapeutic options for melanomas with BRAF mutations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Yongan Ren, Ke Ge, Danyang Sun, Zichen Hong, Changku Jia, Huan Hu, Fangwei Shao, Bo Yao
Summary: A strategy and technology were proposed to assess the lipid amount and membrane protein expression of extracellular vesicles, along with the development of a microfluidic platform for effective EVs enrichment and detection.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Environmental Sciences
Yu-Jen Chiu, Jai-Sing Yang, Fuu-Jen Tsai, Hong-Yi Chiu, Yu-Ning Juan, Yu-Hsiang Lo, Jo-Hua Chiang
Summary: This study identified the resistance of melanoma to vemurafenib and revealed that curcumin can induce apoptosis in vemurafenib-resistant melanoma cells by regulating the EGFR signaling pathway, suggesting curcumin as a potential therapeutic candidate for vemurafenib-resistant melanoma treatment.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Martina Radic, Ignacija Vlasic, Maja Jazvinscak Jembrek, Andela Horvat, Ana Tadijan, Maja Sabol, Marko Duzevic, Maja Herak Bosnar, Neda Slade
Summary: Despite the advancements in melanoma treatment, many patients still develop resistance to drugs, such as the RAF inhibitor vemurafenib. In this study, researchers generated and examined two melanoma-derived cell lines with acquired resistance to vemurafenib. They found that the resistant cells displayed features of epithelial-mesenchymal transition (EMT) and exhibited a hybrid phenotype. Surprisingly, the resistant cells showed decreased migration ability but had a tendency for collective migration. Signaling pathway analysis revealed reactivation of MAPK and activation of the PI3K/AKT pathway in the vemurafenib-resistant cells. The acquired resistance also led to resistance to chemotherapy in the primary melanoma cells. Further investigation is needed to understand the negative regulators of NME proteins in vemurafenib resistance and their impact on PI3K/AKT signaling and melanoma cell characteristics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Xingang Yao, Pin Lyu, Kyung Yoo, Manish Kumar Yadav, Ravi Singh, Anthony Atala, Baisong Lu
Summary: A mechanism to actively enrich RNPs into EVs using RNA aptamer and aptamer-binding protein interactions has been described, resulting in efficient genome editing and transient expression. This system allows for multiplex genome editing targeting multiple loci and can deliver RNPs active in vivo. The data demonstrate that this approach could enhance genome editing efficiency and safety.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Review
Chemistry, Analytical
Qian Liu, Xinmin Yue, Yixian Li, Fang Wu, Meng Meng, Yongmei Yin, Rimo Xi
Summary: The study introduces a novel electrochemical aptasensor based on specific host-guest interactions between cucurbit [7]uril and ferrocene, providing high selectivity and sensitivity in detecting exosomes. The platform allows for specific capture and release of exosomes, showing good performance in exosome detection while maintaining their integrity. This approach can potentially be applied to other target biomolecules by altering the recognition pairs.
Article
Chemistry, Analytical
Wanming Li, Tao Bing, Rui Wang, Sihan Jin, Dihua Shangguan, Hang Chen
Summary: Malignant melanoma is the most aggressive form of skin cancer, with BRAF mutations occurring in 40-50% of cases. Testing for BRAF mutations and targeted therapy have improved long-term survival for patients with BRAF-mutated melanoma. The aptamers CH1 and CH5 show high affinity for BRAF V600E-mutated cells and have potential as molecular probes for detecting metastatic melanoma.
Article
Biochemistry & Molecular Biology
Shujun Han, Mo Zhang, Xiaoyan Qu, Zihao Wu, Zongguan Huang, Yiming Hu, Ying Li, Lanlan Cui, Lu Si, Jiankang Liu, Yongping Shao
Summary: This study identifies a signaling pathway involving SOX10low/TGF-beta/LAMB3/FAK/MMPs that determines the migration and invasion properties of MAPKi-resistant melanoma cells. The LAMB3-Integrinα 3/α 6 signaling mediates the motile and invasive phenotype of resistant cells. SOX10 deficiency in MAPKi-resistant melanoma cells drives LAMB3 upregulation through TGF-beta signaling, and the pro-invasiveness effect of LAMB3 is mediated by the FAK/MMPs axis.
Article
Biochemical Research Methods
Zhenggang Hui, Ming Chang, Mingxian Hu
Summary: This study proposes a novel sEVs sensing system that integrates CD63 aptamer based identification and exonuclease III (Exo-III) assisted signal recycling. The method exhibits a wide detection range and low limit of detection for sEVs, and has been successfully applied for the study of sEVs expression in pneumonia patients and health volunteers.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Nelly Durand, Meline Simsir, Laurie Signetti, Fabien Labbal, Robert Ballotti, Isabelle Mus-Veteau
Summary: The study demonstrated that methiothepin enhances the cytotoxicity of doxorubicin on melanoma cells and overcomes resistance of BRAF(V600E) melanoma cells to vemurafenib and trametinib treatment. This highlights the potential of Ptch1 drug efflux inhibition in improving the effectiveness of anti-cancer treatments for melanoma.
Article
Oncology
Carlotta Guzzetti, Cristina Corno, Elisabetta Vergani, Luca Mirra, Emilio Ciusani, Monica Rodolfo, Paola Perego, Giovanni L. Beretta
Summary: KiSS1, a metastasis suppressor, plays an important role in the development and puberty of melanoma cells. This study found that kisspeptin 54, a cleavage product of KiSS1, can enhance the pro-apoptotic activity of vemurafenib in melanoma cells, including drug-resistant cells. These findings suggest the potential of using KiSS1 to modulate apoptotic response.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yaqi Chen, Anqi Cao, Qinkai Li, JunMin Quan
Summary: In this study, DNA aptamers specifically binding to EBV positive NPC cells were developed using the Cell-SELEX procedure, and the EphA2/CD98hc complex was identified as a potential target. The results revealed a potential function of the EphA2 and CD98hc complex in NPCs.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Hongmei Cui, Qinghui Wang, Duane D. Miller, Wei Li
Summary: This study found that the combination of VERU-111 and Vem has a significant effect on Vem-resistant melanoma, involving molecular signaling pathways such as ERK/AKT and Skp2. This treatment combination holds promise for overcoming Vem resistance in melanoma patients with BRAF (V600E) mutation.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Hui Zhang, Cheng Jin, Lin Zhang, Bo Peng, Yibin Zhang, Yan Liu, Ling Li, Mao Ye, Wei Xiong, Weihong Tan
Summary: The study developed a CD71-specific aptamer targeting the XQ-2d-MMAE conjugate that can distinguish UM cells from normal human uveal melanocytes, suggesting it as a promising novel anti-tumor agent for UM treatment. It demonstrated that CD71 is a reliable target for drug delivery in UM and could be utilized as a model for exploring aptamer-mediated targeted UM treatment strategies.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Yibin Zhang, Jing Dai, Yuan Yang, Junxiao Guo, Lanqin Cao, Mao Ye
Summary: In this study, a high affinity aptamer zyb1 was screened and characterized using infectious cell-SELEX, and a lateral flow strip assay was developed for rapid detection of Mycoplasma hyorhinis. The developed aptasensor showed a detectable limit as low as 1 x 103 CCU/mL, demonstrating its potential for point-of-care testing products for mycoplasma detection.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Li Liu, Hui Li, Dingyu Hu, Yanyan Wang, Wenjun Shao, Jing Zhong, Shudong Yang, Jing Liu, Ji Zhang
Summary: This review explores the complex relationship between m6A methylation and different types of programmed cell death pathways, which are closely associated with the initiation, progression, and resistance of cancer. Clarifying the relationship between m6A and cell death is of great significance for the development of novel strategies for cancer treatment.
Article
Biochemistry & Molecular Biology
Hui Li, Mridul Roy, Long Liang, Wenjie Cao, Bin Hu, Yanan Li, Xiaojuan Xiao, Haiqin Wang, Mao Ye, Shuming Sun, Bin Zhang, Jing Liu
Summary: This study identified the deubiquitylase USP12 plays an important role in pro-survival autophagy and drug resistance in multiple myeloma by stabilizing HMGB1 protein. The USP12/HMGB1 axis might be a potential diagnostic and therapeutic target in human MM.
Article
Cell Biology
Zhaoping Liu, Pan Wang, Shunling Yuan, Yanyan Wang, Pengfei Cao, Feng Wen, Hui Li, Lin Zhu, Long Liang, Zi Wang, Bin Hu, Fuxiang Zheng, Jing Liu, Xiaojuan Xiao, Ji Zhang
Summary: In this study, BC200 was found to be highly expressed in MDS patients and promoted the malignant proliferation of MDS cells through the miR-150-5p/MYB positive feedback loop. This provides evidence for BC200 as a potential therapeutic target in MDS.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Lin Zhu, Qin Yang, Rong Hu, Yanan Li, Yuanliang Peng, Hong Liu, Mao Ye, Bin Zhang, Peihe Zhang, Feng Liu-Smith, Hui Li, Jing Liu
Summary: Albendazole inhibits melanoma cell proliferation through cell cycle arrest and apoptosis, and shows synergistic effects with CDK4/6 inhibitor.
SCIENTIFIC REPORTS
(2022)
Review
Biotechnology & Applied Microbiology
Rong Dong, Saihua Ma, Xiaoli Zhao, Baojuan Wang, Mridul Roy, Lu Yao, Tian Xia, Yanting Liu
Summary: Endometrial injury is a major cause of infertility and current treatments have limitations. Bioinspired hydrogel, with its unique physiochemical properties, shows promising application as a novel delivery platform and alternative therapy for endometrial repair. This review summarizes recent advances in bioinspired hydrogel-based delivery systems for endometrial repair and discusses their current status, limitations, and future perspectives.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yang Sun, Liuting Mo, Xiaoxiao Hu, Die Yu, Sitao Xie, Jianglin Li, Zilong Zhao, Xiaohong Fang, Mao Ye, Liping Qiu, Weihong Tan, Yu Yang
Summary: This study reported a bispecific aptamer that improved T-cell immune response and induced a potent immunological effect against tumors through a recognition-then-conjugation strategy.
Article
Chemistry, Analytical
Hui Zhu, Ende Wu, Zhaoqi Pan, Chi Zhang, Yanchen Zhang, Qianling Liao, Yanan Wang, Yiping Sun, Mao Ye, Wencan Wu
Summary: In this study, a high-affinity DNA aptamer, ZH-1c, was developed to target activated inflammatory microglia. It exhibited promising binding ability under physiological temperatures, high serum stability, and internalization by microglia. The target of ZH-1c on microglia was identified as the transmembrane protein CD64, which increased in response to inflammatory stimulation, enhancing the affinity of ZH-1c for activated microglia. Overall, ZH-1c shows great potential as a novel and effective molecular tool for the diagnosis and microglia-targeted therapies.
ANALYTICAL CHEMISTRY
(2023)
Article
Genetics & Heredity
Nupur Singh, Jude Khatib, Chi-Yang Chiu, Jianjian Lin, Tejesh Surender Patel, Feng Liu-Smith
Summary: This study found that androgen receptor (AR) expression is associated with overall survival in cutaneous melanoma patients. Higher AR protein levels are positively associated with better overall survival. The association between AR and overall survival is significant in female patients but not in males. AR is also associated with overall survival in RAS mutant subtypes of melanoma.
Article
Obstetrics & Gynecology
Joan Lien, Tristan Hayes, Feng Liu-Smith, Divya Rana
Summary: This cross-sectional observational study found a significant increase in maternal fentanyl and tobacco use during the COVID-19 pandemic compared to pre-pandemic. It also identified an increase in preterm births and lower birth weights among infants born to mothers with substance use.
JOURNAL OF PERINATOLOGY
(2023)
Article
Dermatology
Victoria Collier, Jay Patel, Chi-Yang Chiu, Jay H. Fowke, Tejesh Surendr Patel, Feng Liu-Smith
Summary: There is a significant difference in the bodily distribution of cutaneous melanomas (CMs) between men and women, with CMs more frequently found on the trunk in men and on the hip and lower extremities in women. This difference is believed to be due to greater exposure to ultraviolet radiation for women's legs. However, this study found that white men have lower rates and lower relative tumor density (RTD) of CMs on their legs compared to other body sites or compared to white women. White women had comparable RTDs among different body sites. Tumor mutational burdens (TMBs) for leg melanomas were also lower in both sexes. This suggests that the legs of both sexes, especially in white patients, have a weaker association with UV exposure and a distinct protective effect against CMs.
CLINICAL AND EXPERIMENTAL DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ottis Scrivner, Long Dao, M. Karen Newell-Rogers, Babbak Shahandeh, Frank L. Meyskens, Susan Kurumi Kozawa, Feng Liu-Smith, German Plascencia-Villa, Miguel Jose-Yacaman, Shang Jia, Christopher J. Chang, Patrick J. Farmer
Summary: This report investigates the toxicity of the ionophore thiomaltol (Htma) and Cu salts to melanoma. Results show that the divalent metal complexes of thiomaltol display higher toxicity against melanoma cells, with Cu(tma)(2) > Zn(tma)(2) >> Ni(tma)(2). The accumulation of copper within lysosomes is found to be associated with the onset of apoptosis.