Article
Biochemical Research Methods
Barbara Radovani, Gordan Lauc, Ivan Gudelj
Summary: Immunoglobulin G (IgG) is an important antibody in the blood that plays a critical role in the immune defense against infectious agents. The glycosylation of IgG has been found to affect its functions and is associated with disease development and progression. Saliva is an easily collectible sample and could be a useful tool for studying the glycosylation of salivary IgG and its potential as a diagnostic biomarker. This study describes a method for analyzing the glycosylation of IgG from saliva samples using ultra-high-performance liquid chromatography, and compares it with the glycosylation profiles of IgG from plasma. The stability of salivary IgG glycosylation under different storage conditions and the effects of using a saliva preservation medium were also assessed.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Anne-Lise Marie, Somak Ray, Shulin Lu, Jennifer Jones, Ionita Ghiran, Alexander R. Ivanov
Summary: A highly sensitive method for profiling N-glycans released from proteins using CZE-ESI-MS was developed and applied to glycan analysis of plasma and blood-derived isolates. The technique significantly improved detection sensitivity and resolution, allowing for qualitative and quantitative analysis of N-glycans in blood samples with unmatched depth compared to previous methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Zhanchen Guo, Yang Chen, Shuangshou Wang, Jilei Pang, Zhen Liu
Summary: The use of nanomaterials like n-octyl-modified monodispersed dendritic mesoporous silica nanospheres (DMSNs) with small diameter and appropriate pore size has shown great potential in improving separation efficiency in liquid chromatography. The unique morphology of DMSNs allows for highly uniform packing and ultrahigh efficiency, making them a promising candidate for next-generation packing materials. The introduction of DMSNs has opened up new possibilities for achieving ultraefficient liquid chromatographic separations.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Medicine, Research & Experimental
Danqi Li, Yuchen Lou, Yamin Zhang, Si Liu, Jun Li, Juan Tao
Summary: Human immunoglobulin G (IgG), especially sialylated IgG, plays a crucial role in the diagnosis and management of autoimmune diseases by exerting significant anti-inflammatory effects. Current research indicates that IgG Fc N-glycosylations are more reliable diagnostic and monitoring biomarkers than total IgG autoantibodies. IgG sialylation-based therapies show promise in attenuating pathogenic autoimmunity in preclinical and clinical studies.
Article
Chemistry, Analytical
Cuiyan Cao, Long Yu, Jingyu Yan, Dongmei Fu, Jingli Yuan, Xinmiao Liang
Summary: A purification strategy based on two-dimensional hydrophilic interaction liquid chromatography and porous graphitized carbon chromatography was established to obtain neutral N-glycan standards from natural sources. The purified N-glycans with defined structures, purity, and amounts were successfully assembled on glass slides, demonstrating the potential of the strategy in glycan library expansion and functional glycomics. The detailed structures of the N-glycan compounds were verified through negative ion tandem MS analysis, allowing for identification of isomeric and terminal fine structures.
Article
Biotechnology & Applied Microbiology
Sumit K. Singh, Kelvin H. Lee
Summary: Glycosylation is a critical quality attribute of monoclonal antibody therapeutics. Hydrophilic interaction liquid chromatography-mass spectrometry (HILIC-MS) is a valuable technology for glycosylation characterization. Traditional glycan analysis based on glucose units (GU) and accurate mass (AM) is not suitable for complex samples or high-throughput applications. This study presents a two-step strategy for targeted glycan analysis and its application in different scenarios.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Konstantinos Flevaris, Cleo Kontoravdi
Summary: This study provides a critical overview of human IgG N-glycomics, focusing on identifying disease-specific glycan alterations. The use of advanced computational tools to interpret clinical data and their relationship with molecular mechanisms is crucial for establishing clinically-relevant N-glycan biomarkers. Glycoinformatics tools, such as artificial intelligence and systems glycobiology approaches, have the potential to provide insight into patient stratification and disease etiology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hae Gon Lee, Shinill Kang, Joon Sang Lee
Summary: Protein-protein interactions and their binding are crucial for biological metabolism. Understanding the interactions between IgG and natural immunoglobulin-binding ligands is essential for pharmaceutical science and biotechnology. This study analyzed the binding strengths of IgG-spA and IgG-spG complexes and identified the mechanisms enabling these bindings.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemical Research Methods
Rebeca Kawahara, Anastasia Chernykh, Kathirvel Alagesan, Marshall Bern, Weiqian Cao, Robert J. Chalkley, Kai Cheng, Matthew S. Choo, Nathan Edwards, Radoslav Goldman, Marcus Hoffmann, Yingwei Hu, Yifan Huang, Jin Young Kim, Doron Kletter, Benoit Liquet, Mingqi Liu, Yehia Mechref, Bo Meng, Sriram Neelamegham, Terry Nguyen-Khuong, Jonas Nilsson, Adam Pap, Gun Wook Park, Benjamin L. Parker, Cassandra L. Pegg, Josef M. Penninger, Toan K. Phung, Markus Pioch, Erdmann Rapp, Enes Sakalli, Miloslav Sanda, Benjamin L. Schulz, Nichollas E. Scott, Georgy Sofronov, Johannes Stadlmann, Sergey Y. Vakhrushev, Christina M. Woo, Hung-Yi Wu, Pengyuan Yang, Wantao Ying, Hui Zhang, Yong Zhang, Jingfu Zhao, Joseph Zaia, Stuart M. Haslam, Giuseppe Palmisano, Jong Shin Yoo, Goran Larson, Kai-Hooi Khoo, Katalin F. Medzihradszky, Daniel Kolarich, Nicolle H. Packer, Morten Thaysen-Andersen
Summary: The study evaluated the performance of glycoproteomics software, identifying high-performance informatics strategies and recommending improvements for search solutions.
Article
Chemistry, Analytical
Chia Yen Liew, Hong-Sheng Luo, Ting-Yi Yang, An-Ti Hung, Bryan John Abel Magoling, Charles Pin-Kuang Lai, Chi-Kung Ni
Summary: This study re-examines high mannose N-glycans in multicellular eukaryotes using a new mass spectrometry method, and identifies many previously unreported isomers. A database is constructed for rapid identification of high mannose N-glycan isomers.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yao-Yao Zhang, Mattia Ghirardello, Ting Wang, Ai-Min Lu, Li Liu, Josef Voglmeir, M. Carmen Galan
Summary: The novel imidazolium derivative (GITag) enhances ionisation efficiency for improved mass spectrometric detection of oligosaccharides and N-glycans. Direct labelling of human serum samples with GITag on a MALDI target plate eliminates the need for sample pretreatment or clean-up steps.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biophysics
Atsushi Hirano, Shogo Kanoh, Kentaro Shiraki, Momoyo Wada, Masahiro Kitamura, Katsuya Kato
Summary: This study discovered that phosphate-modified zirconia nanoparticles can selectively adsorb immunoglobulin G (IgG) antibodies with high capacity while maintaining their molecular conformation. Compared to traditional Protein A ligands, zirconia nanoparticles have higher affinity for human IgG antibodies, especially IgG3. Due to their chemical and mechanical stability, zirconia nanoparticles can be used in batch methods and chromatography as an alternative to Protein A chromatography for the purification of antibody drugs.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Biochemical Research Methods
Katarina Molnarova, Ales Duris, Tomas Jecmen, Petr Kozlik
Summary: Glycoproteomics, a challenging field due to glycosylation heterogeneity, benefits from hydrophilic interaction liquid chromatography for glycopeptide separation. Comparing HILIC columns in human immunoglobulin G glycopeptide separation, it was found that different columns exhibit higher separation power for different glycoforms and can effectively elucidate the structure of isomeric glycoforms. The retention mechanism for HILIC columns is discussed based on determined chromatographic parameters.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Hsiao-Fan Chen, Chih-Chin Kao, Shuk-Man Ka, San-Yuan Wang, Michael X. Chen, Guan-Yuan Chen, Te- Weng, Ruo-Yun Lai, Shu-Ching Yeh, Yen-Chung Lin, Hsi-Hsien Chen, Wei-Chiao Chang, Mai-Szu Wu, I-Lin Tsai
Summary: An analytical method was developed to identify plasma biomarkers for IgA nephropathy. The method involved the analysis of IgA1 hinge region glycoforms and revealed a decrease in sialylation in the IgA1 hinge region of IgAN patients, which was correlated with the estimated glomerular filtration rate.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Applied
Jingshun Zhang, Qin Chen, Ying Zhou, Yunzhen Zhang, Yiping Ren, Lianliang Liu
Summary: This study established an ultra-high performance liquid chromatography mass spectrometry method for the quantitative determination of bovine IgG subtypes in dairy products. The method utilized high-resolution mass spectrometry to confirm the specificity and stability of theoretical peptides. Characteristic peptides were screened for the quantitative analysis of bovine IgG subtypes. Isotope-labeled peptides were used to correct matrix effects. The method showed high accuracy and reliability.
Article
Biochemistry & Molecular Biology
Di Liu, Youxin Wang, Huiquan Jing, Qun Meng, Jingyun Yang
Summary: This study identified multiple DNA methylation sites and genes showing pleiotropic associations with Alzheimer's dementia through network SMR analysis, revealing potential causal pathways and shedding light on the role of DNA methylation in the development of Alzheimer's dementia.
Article
Public, Environmental & Occupational Health
Yunfeng Xi, Qiuyue Tian, Buqi Na, Ke Han, Mingrui Duan, Xingguang Zhang, Wenrui Wang, Youxin Wang
Summary: As an ongoing cohort study, IMAGINS aims to explore risk factors for cardiovascular diseases and evaluate the effectiveness of health management for high-risk population. This study features detailed data collection and long-term follow-up, providing a great research opportunity for the prevention and control of cardiovascular diseases.
Article
Environmental Sciences
Mengyang Liu, Zhiwei Li, Mengmeng Liu, Yingxuan Zhu, Yue Liu, Mandela William Nzoyoum Kuetche, Jianpeng Wang, Xiaonan Wang, Xiangtong Liu, Xia Li, Wei Wang, Xiuhua Guo, Lixin Tao
Summary: The study revealed a correlation between temperature and daily new COVID-19 cases, showing a positive relationship at low temperatures and a negative relationship at high temperatures. The findings suggest temperature levels may affect the spread of the virus in different regions.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Medicine, Research & Experimental
Qiuyue Tian, Biyan Wang, Shuohua Chen, Shouling Wu, Youxin Wang
Summary: Long-term trajectory analysis shows that moderate physical activity may not decrease the risk of cardiovascular disease (CVD) in persistently overweight and obese adults, but moderate physical activity in the normal weight status and persistent overweight status is associated with decreased risk of all-cause mortality.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Medicine, General & Internal
Xiaoni Meng, Fei Wang, Xiangyang Gao, Biyan Wang, Xizhu Xu, Youxin Wang, Wei Wang, Qiang Zeng
Summary: This study aims to explore the relationship between suboptimal health status (SHS) and type 2 diabetes mellitus (T2DM) by examining the characteristics of IgG N-glycans. The study found significant associations between IgG N-glycan traits and T2DM. Combining IgG N-glycans with clinical features, a combined model was developed that could better differentiate between T2DM patients and healthy individuals. The findings suggest that early detection of T2DM risk individuals and personalized prevention and treatment can be achieved through the detection of IgG N-glycans.
Letter
Endocrinology & Metabolism
Xiaoni Meng, Youxin Wang
Article
Endocrinology & Metabolism
Haotian Wang, Yuan Li, Weijie Cao, Jie Zhang, Mingyang Cao, Xiaoni Meng, Di Liu, Youxin Wang
Summary: This study examines the relationship between type 2 diabetes (T2D) and hypertension, finding evidence of a bidirectional association. They also explore the role of IgG N-glycosylation in linking these two conditions and identify several genetic variants associated with both T2D and hypertension. The results provide further support for the common soil hypothesis underlying the pathogenesis of T2D and hypertension.
DIABETOLOGY & METABOLIC SYNDROME
(2023)
Article
Medicine, General & Internal
Qiuyue Tian, Shuohua Chen, Jie Zhang, Cancan Li, Shouling Wu, Yanxiu Wang, Youxin Wang
Summary: This study investigates the association between cardiovascular health (CVH) metrics and life expectancy in participants without cardiovascular diseases (CVDs). The findings suggest that a good CVH status is associated with a lower risk of premature mortality and a longer disease-free life expectancy. Therefore, promoting a higher CVH score or ideal CVH status can reduce the burden of CVD events and extend disease-free life expectancy, providing accurate predictions for personalized medicine.
Article
Engineering, Multidisciplinary
Julija Juric, Hongli Peng, Manshu Song, Frano Vuckovic, Jelena Simunovic, Irena Trbojevi-Akmacic, Youxin Wang, Jiaonan Liu, Qing Gao, Hao Wang, Qiaoyun Chu, Marija Pezer, Wei Wang, Gordan Lauc
Summary: The N-glycosylation of IgG exhibits cyclic periodic changes during the menstrual cycle, which are associated with the menstrual cycle phase and sex hormone concentration. While the average effect of the menstrual cycle on IgG N-glycosylation traits is low, there are significant intrapersonal variations. These findings are important for large sample-size studies on IgG N-glycosylation in women.
Editorial Material
Oncology
Jun Wen, Wei Wang
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Medicine, Research & Experimental
Qiuyue Tian, Shuohua Chen, Xiaoni Meng, Haotian Wang, Cancan Li, Deqiang Zheng, Lijuan Wu, Aitian Wang, Shouling Wu, Youxin Wang
Summary: This study found that maintaining a higher cardiovascular health (CVH) score can reduce the risk of cardiovascular diseases and mortality. The study also found that the duration of maintaining a favorable CVH status is associated with the risk of developing cardiovascular diseases and mortality. The results indicate that maintaining a better CVH status for more than 6 years can lower the risk of cardiovascular diseases and mortality.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Qiaoyun Zhang, Guangheng Wu, Xiaoyu Zhang, Sheng Wang, Youxin Wang
Summary: This study used Mendelian randomization to explore the causal effect of atherosclerosis related indicators on dementia risk. The analysis of genome-wide association studies data revealed an inverse association between these indicators and the risk of dementia. Sensitivity analysis also confirmed this association for a specific type of dementia. Further research with larger sample sizes is needed to validate these findings.
Article
Environmental Studies
Danni Zhenga, Jun Wenb, Metin Kozakc, Ian Phaud, Haifeng Houe, Wei Wang
Summary: Research on travel-eligible tourists with medical conditions, such as dementia, is lacking in the tourism literature. This paper highlights the challenges of conducting empirical research on this population and suggests solutions like clear recruitment criteria and randomized controlled trials. The resultant studies can inform tourism management and marketing strategies.
TOURISM MANAGEMENT
(2023)
Letter
Biotechnology & Applied Microbiology
Xuerui Tan, Wei Wang
OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
(2022)
