期刊
EMERGING MICROBES & INFECTIONS
卷 9, 期 1, 页码 508-516出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/22221751.2020.1732231
关键词
Colistin resistance; mcr; mcr-10; plasmid; Enterobacter roggenkampii
资金
- National Natural Science Foundation of China [81772233, 81661130159, 81861138055]
- West China Hospital of Sichuan University [ZYYC08006]
- Newton Advanced Fellowship, Royal Society, UK [NA150363]
Mobile colistin resistance (mcr) genes represent an emerging challenge. Here we describe a novel mcr gene, mcr-10, on an IncFIA plasmid of an Enterobacter roggenkampii clinical strain. mcr-10 has the highest nucleotide identity (79.69%) with mcr-9 and encodes MCR-10 with 82.93% amino acids identical to MCR-9. mcr-10 confers 4-fold increase in colistin MIC (from 1 to 4 mg/L) when cloned into a colistin-susceptible E. roggenkampii strain. By screening GenBank, mcr-10 was found in various Enterobacteriaceae species of countries in four continents, suggesting that this gene has widely spread. MCR-10 shows 79.04% to 83.67% amino acid identity and highly conserved predicted protein structures with chromosomally encoded MCR-like phosphoethanolamine transferases (designated MCR-B here) of various Buttiauxella species. MCR-10, MCR-9 and MCR-B proteins may, therefore, originate from a common ancestor. mcr-10 was adjacent to a site-specific recombinase-encoding gene and was bracketed by IS903 and may be mobilized by site-specific recombination or composite transposon. Our results indicate that mcr-10 is a novel plasmid-borne colistin resistance gene and warrants immediate monitoring and further studies.
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