期刊
MICROMACHINES
卷 11, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/mi11030246
关键词
micro-stereolithography; cytotoxicity; resin development; microfluidics; cell culture; human umbilical vein endothelial cells (HUVECs); biocompatibility; 3D printing; additive manufacturing
类别
资金
- Federal Ministry of Education and Research (BMBF
- Biotechnology2020+: Leibniz Research Cluster) [031A360C]
- Volkswagen Foundation (Experiment!)
- German Research Foundation (DFG, Research Training Group 1865: Hydrogel-based Microsystems)
Three-dimensional (3D) printing of microfluidic devices continuously replaces conventional fabrication methods. A versatile tool for achieving microscopic feature sizes and short process times is micro-stereolithography (mu SL). However, common resins for mu SL lack biocompatibility and are cytotoxic. This work focuses on developing new photo-curable resins as a basis for mu SL fabrication of polymer materials and surfaces for cell culture. Different acrylate- and methacrylate-based compositions are screened for material characteristics including wettability, surface roughness, and swelling behavior. For further understanding, the impact of photo-absorber and photo-initiator on the cytotoxicity of 3D-printed substrates is studied. Cell culture experiments with human umbilical vein endothelial cells (HUVECs) in standard polystyrene vessels are compared to 3D-printed parts made from our library of homemade resins. Among these, after optimizing material composition and post-processing, we identify selected mixtures of poly(ethylene glycol) diacrylate (PEGDA) and poly(ethylene glycol) methyl ethyl methacrylate (PEGMEMA) as most suitable to allow for fabricating cell culture platforms that retain both the viability and proliferation of HUVECs. Next, our PEGDA/PEGMEMA resins will be further optimized regarding minimal feature size and cell adhesion to fabricate microscopic (microfluidic) cell culture platforms, e.g., for studying vascularization of HUVECs in vitro.
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