期刊
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2019.00413
关键词
peripheral blood mononuclear cells; Toxoplasma; ROP16 protein; ROP18 protein; ocular toxoplasmosis; cytokines; polymorphisms
资金
- COLCIENCIAS [1113-744-55483, 1110-569-34589]
- Universidad del Quindio
- Universidad Tecnologica de Pereira [5-14-1, 5-11-13]
Toxoplasma gondii ROP16 and ROP18 proteins have been identified as important virulence factors for this parasite. Here, we describe the effect of ROP16 and ROP18 proteins on peripheral blood mononuclear cells (PBMCs) from individuals with different clinical status of infection. We evaluated IFN-gamma, IL-10, and IL-1 beta levels in supernatants from PBMCs cultures infected with tachyzoites of the T. gondii wild-type RH strain or with knock-out mutants of the rop16 and rop18 encoding genes (RH Delta rop16 and RH Delta rop18). Cytokine secretion was compared between PBMCs obtained from seronegative individuals (n = 10), with those with chronic asymptomatic (n = 8), or ocular infection (n = 12). We also evaluated if polymorphisms in the genes encoding for IFN-gamma, IL-10, IL-1 beta, Toll-like receptor 9 (TLR9), and purinoreceptor P2RX7 influenced the production of the encoded proteins after ex vivo stimulation. In individuals with chronic asymptomatic infection, only a moderate effect on IL-10 levels was observed when PBMCs were infected with RH Delta rop16, whereas a significant difference in the levels of inflammatory cytokines IFN-gamma and IL-1 beta was observed in seronegative individuals, but this was also dependent on the host's cytokine gene polymorphisms. Infection with ROP16-deficient parasites had a significant effect on IFN-gamma production in previously non-infected individuals, suggesting that ROP16 which is considered as a virulence factor plays a role during the primary infection in humans, but not in the secondary immune response.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据