期刊
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷 44, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.algal.2019.101685
关键词
Phycobiliprotein purification; Membrane chromatography; Hydrophilic microfiltration polyvinylidene; fluoride (PVDF) membrane; Ammonium sulphate; Arthrospira; Porphyridium
资金
- H2020 project SABANA from the European Union's Horizon 2020 research and innovation program [727874]
Membrane chromatography was exploited to purify allophycocyanin, phycocyanin and B-phycoerythrin from aqueous extracts of fresh or freeze-dried biomass of Arthrospira platensis (Cyanobacteria) and Porphyridium cruentum (Rhodophyta). The method can be tuned to obtain products having various degree of purity and avoids expensive and time consuming column chromatography and ultrafiltration steps. A commercial polyvinylidene fluoride (PVDF) microfiltration membrane (hydrophilic and low protein binding) was used as an ammonium sulphate responsive stationary phase to carry out the purification process. Analytical grade purity was achieved for phycocyanin (purity = 4.2-4.5, yield = 75-82 %) and B-phycoerythrin (purity = 4.5-4.8, yield = 69-71 %). Good purification, even if lower than analytical grade, was achieved for allophycocyanin (purity = 3.3-3.7, yield = 42-47%).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据