Article
Agricultural Engineering
Baobei Wang, Xueshan Pan, Fang Wang, Lulu Liu, Jing Jia
Summary: This study analyzed the biochemical compositions and proteomics of mixotrophic Haematococcus pluvialis under different light conditions. The findings showed that high light exposure increased astaxanthin, carbohydrate, and fatty acid contents, and regulated multiple metabolic pathways. A regulatory model for mixotrophic Haematococcus pluvialis coping with high light stress was proposed.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Qunju Hu, Danqiong Huang, Anguo Li, Zhangli Hu, Zhengquan Gao, Yongli Yang, Chaogang Wang
Summary: This study analyzed the transcriptome changes in Haematococcus pluvialis under exogenous salicylic acid and high light stresses, identifying genes associated with metabolite biosynthesis, photosynthesis, and immune system responses. The results suggest a coordinated relation between astaxanthin and fatty acid biosynthesis, with salicylic acid potentially playing a role in the self-protection processes of cells in adapting to high light stress.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Agricultural Engineering
Ha Eun Yang, Byung Sun Yu, Sang Jun Sim
Summary: The aim of this study was to increase the productivity of biomass with high astaxanthin content by developing a mutant strain of Haematococcus pluvialis with strong environmental tolerance. The mutant M5 strain, selected through gamma irradiation, showed improved biomass and astaxanthin production under high-salinity and high-light intensity conditions. M5 demonstrated an increase in biomass and astaxanthin productivity by 86.70% and 66.15% respectively, compared to untreated cells.
BIORESOURCE TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Petras Venckus, Ieva Endriukaityte, Kotryna Cekuolyte, Renata Gudiukaite, Andrius Pakalniskis, Egle Lastauskiene
Summary: This study tested the effects of silver nanoparticles on Haematococcus pluvialis and found that high concentrations of AgNPs significantly reduced culture productivity in green-stage cultures, while the impact on red-stage cultures was smaller. The addition of AgNPs also led to changes in cell morphology, including deformations, disrupted cytokinesis, and induced sexual reproduction.
Review
Biotechnology & Applied Microbiology
Adolf Acheampong, Lamei Li, Shereen M. Elsherbiny, Yahui Wu, Mohammed Sharif Swallah, Precious Bondzie-Quaye, Qing Huang
Summary: This review investigates strategies to enhance Astaxanthin production from H. pluvialis, focusing on genetic and environmental factors and transcriptomic dynamics. Genetic engineering and culture optimization are effective approaches to increase Astaxanthin production, while new technologies may become principal strategies in the future.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Oleksandr Virchenko, Tryggvi Stefansson
Summary: This study aimed to assess the effect of light on the spectrometric and high-performance liquid chromatography (HPLC) measurements of astaxanthin. The results showed that after 3 hours of light exposure, astaxanthin absorbance exhibited logarithmic growth of 8-11% (UV/Vis) and 7-17% (HPLC).
Article
Fisheries
Feichao Du, Chaoyang Hu, Xue Sun, Nianjun Xu
Summary: The addition of sucrose in Haematococcus pluvialis significantly increases astaxanthin accumulation, by upregulating genes involved in sucrose and starch metabolism, glycolysis, astaxanthin biosynthesis, and fatty acids metabolism. This study demonstrates the potential of sucrose treatment in promoting astaxanthin biosynthesis and shifting carbon metabolism towards astaxanthin accumulation in H. pluvialis under high light stress conditions.
Article
Biochemistry & Molecular Biology
Jinpeng Sun, Jiawei Zan, Xiaonan Zang
Summary: This study investigates the effect of fluridone on the biomass, chlorophyll, and astaxanthin content of Haematococcus pluvialis, as well as its regulation on gene expression in several metabolic pathways. The results demonstrate that fluridone can affect the accumulation of astaxanthin and growth of Haematococcus pluvialis by modulating the synthesis of carotenoids, chlorophyll, fatty acids, and other compounds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Green & Sustainable Science & Technology
Gannoru Kankanamalage Sanuji Hasara Nishshanka, Vinoj Chamilka Liyanaarachchi, Pemaththu Hewa Viraj Nimarshana, Thilini Udayangani Ariyadasa, Jo-Shu Chang
Summary: The microalga Haematococcus pluvialis has high biotechnological potential for producing various valuable compounds. Biorefining, which integrates multiple production processes in a single facility, can maximize the utilization of feedstock and reduce resource consumption. This review discusses the potential of biorefining H. pluvialis to promote more sustainable and cleaner production technologies.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Agricultural Engineering
Yongteng Zhao, Hui-Ping Wang, Chunli Yu, Wei Ding, Benyong Han, Shuxiang Geng, Delu Ning, Ting Ma, Xuya Yu
Summary: The study revealed that melatonin treatment upregulated the biosynthesis of astaxanthin and lipids in Haematococcus pluvialis, affecting the accumulation process through the regulation of gene expression and metabolic pathways. The treatment may promote coproduction of astaxanthin and lipids by regulating the carbon-nitrogen balance and antioxidant system.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Khadija Samhat, Antoinette Kazbar, Hosni Takache, Ali Ismail, Jeremy Pruvost
Summary: This study elucidates the role of light transfer in astaxanthin production in Haematococcus pluvialis during nitrogen starvation. The results demonstrate the importance of the mean rate of photon absorption (MRPA) in triggering the accumulation of astaxanthin and introduce MRPA as a useful parameter for optimizing light culture conditions in photobioreactors (PBRs).
BIORESOURCES AND BIOPROCESSING
(2023)
Article
Agricultural Engineering
Aye Aye Myint, Patrick Hariyanto, Muhammad Irshad, Cao Ruqian, Sabrinna Wulandari, Min Eui Hong, Sang Jun Sim, Jaehoon Kim
Summary: A novel integrated extraction technique was demonstrated to achieve high recovery of natural astaxanthin from wet encysted Haematococcus pluvialis without the need for a pre-drying step. By researching the most suitable green solvent and optimizing the extraction conditions, a high recovery of astaxanthin and extraction yield were achieved. The biphasic solvent system played a key role in this process.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jae Been Lee, Yun Hwan Park, Min Seo Jeon, Sok Kim, Yoon-E Choi
Summary: In this study, the researchers successfully increased the production of astaxanthin in Haematococcus pluvialis by combining polyethylenimine (PEI) with chitosan. The polyethylenimine concentration had an effect on astaxanthin accumulation, while the molecular weight of chitosan did not. The treatment also increased the reactive oxygen species content in cells, leading to the upregulation of enzymes involved in astaxanthin biosynthesis. The PEI-chitosan fiber treatment showed high recyclability and maintained over 90% astaxanthin production efficiency.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Chaogang Wang, Kunpeng Wang, Jingjing Ning, Qiulan Luo, Yi Yang, Danqiong Huang, Hui Li
Summary: This study identified different transcription factors involved in the regulation of astaxanthin synthesis in Haematococcus pluvialis through transcriptome analysis, providing new insights into the regulatory mechanism of astaxanthin synthesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
Hang Guo, Tao Li, Yongteng Zhao, Xuya Yu
Summary: Copper treatment significantly increased astaxanthin and lipid synthesis in Haematococcus pluvialis, leading to upregulation of biosynthesis genes and antioxidant enzyme-related genes, as well as a decrease in reactive oxygen species levels. Additionally, copper induced the activation of protein kinases, facilitating the production of astaxanthin and lipids in the algae.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
Lamei Li, Zhu Chen, Adolf Acheampong, Qing Huang
Summary: This study investigates the mechanism of low-temperature plasma (LTP) in promoting astaxanthin yield in Haematococcus pluvialis, showing that LTP treatment increases levels of strigolactone and abscisic acid, leading to enhanced expression of astaxanthin synthesis genes, especially CRTISO. The up-regulation of CRTISO is linked to increased H3K4me3 in its promoter region, demonstrating that LTP regulates gene expression and astaxanthin accumulation through phytohormones and epigenetic modifications.
BIORESOURCE TECHNOLOGY
(2022)
Article
Spectroscopy
Guohua Yao, Shuju Guo, Wenjie Yu, Muhammad Muhammad, Jianguo Liu, Qing Huang
Summary: Astaxanthin is a xanthophyll carotenoid widely found in aquatic animals, with various physiological functions. Different optical isomers of astaxanthin have differences in physiological activities. Raman spectroscopy can distinguish the three optical isomers of astaxanthin.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Review
Food Science & Technology
Mohammed Sharif Swallah, Xiaoqing Yang, Jiaxin Li, Joseph Kudadam Korese, Sainan Wang, Hongliang Fan, Hansong Yu, Qing Huang
Summary: Glycine max, an economically important grain crop, has varied potential health benefits. Recent studies have focused on the bioavailability and bioaccessibility of soybean bioactive compounds. This review presents evidence-based information on the therapeutic and nutritional importance of soybean phytochemicals and their implications on general health. The bioavailability, metabolism, and safety of soybean phytochemicals are also discussed, as well as the need for clinical trials to confirm these functional properties in humans.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Spectroscopy
Guohua Yao, Qing Huang
Summary: Acetylation is a common and important protein modification. This study explored the vibrational assignments and geometric structures of acetylated lysines through Raman and infrared spectroscopy, providing guidance for detecting protein acetylation using spectroscopic techniques.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Chao Liu, Shenhao Wang, Xuan Dong, Qing Huang
Summary: This study proposes a simple and rapid method for in situ detection of pesticide residues on fruits and vegetables using surface-enhanced Raman spectroscopy (SERS). The flexible and transparent SERS substrates can be easily pasted onto the surface of agricultural products for residue detection. The experimental results show excellent detection performance and repeatability.
Review
Biochemistry & Molecular Biology
Mohammed Sharif Swallah, Precious Bondzie-Quaye, Yahui Wu, Adolf Acheampong, Frederick Leo Sossah, Shereen M. M. Elsherbiny, Qing Huang
Summary: Ganoderma lucidum (G. lucidum) is a traditional Asian medicine with a long history, known for its health-improving and life-extending properties. It contains bioactive metabolites such as polysaccharides, triterpenoids, and polyphenols, which have antioxidant, anti-inflammatory, anticancer, hepatoprotective, and antidiabetic activities. This mushroom species is widely used in the food industry as a nutrient supplement.
Article
Fisheries
Xinxin Zheng, Changsheng Shao, Jianxia Zhu, Leisheng Zhang, Qing Huang
Summary: Through experiments on normal cells, it was found that astaxanthin can significantly enhance the cell viability under gamma-ray radiation conditions by reducing reactive oxygen species (ROS) in the cells and alleviating radiation-induced DNA damage. Furthermore, astaxanthin was found to activate the Nrf2 signaling pathway and upregulate antioxidant proteins HO-1 and p-Nrf2, which helps alleviate oxidative stress caused by ionizing radiation. This study not only provides evidence of the outstanding antioxidant properties of astaxanthin, but also expands its potential applications in radiation protection.
AQUACULTURE INTERNATIONAL
(2023)
Article
Chemistry, Multidisciplinary
Shereen M. Elsherbiny, Mahmoud A. Khalifa, Adolf Acheampong, Chao Liu, Precious Bondzie-Quaye, Mohammed S. Swallah, Xiuping Lin, Qing Huang
Summary: The potential of Bi2MoO6 nanoparticles as a photocatalyst for decomposing organic pollutants has been widely explored, but their potential for photodynamic therapy (PDT) has not been investigated. In this study, a novel nanocomposite based on Bi2MoO6/ MoS2/AuNRs was designed to overcome the limitation of UV absorption. The nanocomposite exhibited high photodynamic ability and POD-like activity under NIR-II light irradiation.
Review
Mycology
Precious Bondzie-Quaye, Mohammed Sharif Swallah, Adolf Acheampong, Shereen M. Elsherbiny, Edward Opong Acheampong, Qing Huang
Summary: Ganoderic acids (GA), secondary metabolites from Ganoderma lucidum, have diverse pharmacological activities and therapeutic effects. However, they are produced at low concentrations and require tedious cultivation. The elucidation of GA biosynthesis regulatory mechanisms has garnered interest, and strategies to control production and increase yield are explored. This review discusses insights into environmental and genetic regulation of GA biosynthesis and challenges in understanding GA diversification.
MYCOLOGICAL PROGRESS
(2023)
Article
Engineering, Environmental
Cao Fang, Changsheng Shao, Shenhao Wang, Yahui Wu, Chao Liu, Qing Huang
Summary: Antibiotics are widely used and their discharge into the environment poses a constant potential threat to the ecosystem. This study focused on the treatment of wastewater containing levofloxacin and sulfadiazine, two typical antibiotics, using atmospheric-pressure dielectric barrier discharge (DBD). The results showed that the total antibiotics removal efficiency could be improved compared to separate single antibiotic treatment. The role of plasma-induced reactive oxygen/nitrogen species (RONS) was examined, with hydroxyl radicals (OH) playing a major role in levofloxacin degradation and ozone and peroxynitrite also contributing to sulfadiazine degradation. The bio-toxicity evaluation demonstrated the safety of the DBD degradation products for the ecological environment.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2023)
Article
Biochemistry & Molecular Biology
Mengmeng Duan, Yalin Li, Fengqiu Zhang, Qing Huang
Summary: This study induced the transformation of B-DNA to Z-DNA using NaCl, MgCl2, and ethanol, and monitored the transformation process using Fourier transform infrared (FTIR) spectroscopy. The structural changes were examined, and the DNA chirality alterations were validated by circular dichroism (CD) measurements. Z-DNA characteristic signals were observed in the IR spectrum, and the intensity ratios increased with Z-DNA content. Compared to CD spectra, IR spectra showed higher sensitivity to Z-DNA and provided more information about the molecular structure change. In conclusion, this study established a reliable FTIR analytical approach to assess BZ DNA conformational changes, which can help understand the Z-DNA transition mechanism and study Z-DNA functions in biological systems.
Review
Biotechnology & Applied Microbiology
Adolf Acheampong, Lamei Li, Shereen M. Elsherbiny, Yahui Wu, Mohammed Sharif Swallah, Precious Bondzie-Quaye, Qing Huang
Summary: This review investigates strategies to enhance Astaxanthin production from H. pluvialis, focusing on genetic and environmental factors and transcriptomic dynamics. Genetic engineering and culture optimization are effective approaches to increase Astaxanthin production, while new technologies may become principal strategies in the future.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Guohua Yao, Chao Liu, Shereen M. Elsherbiny, Qing Huang
Summary: Ribose, the central unit in RNA, plays a crucial role in studying the origin of life and the chiral homogeneity of biological molecules. However, traditional chiral recognition methods for ribose are time-consuming, expensive, and limited to laboratory settings. In this study, a simple and convenient approach utilizing colorimetry and SERS was developed to quickly detect the chirality of ribose, providing a faster, cost-effective, and portable solution.
Article
Chemistry, Analytical
Fengqiu Zhang, Changsheng Shao, Yahui Wu, Wei Zhao, Xumiao Jing, Cao Fang, Qing Huang
Summary: In this study, a GFP fluorescent probe was utilized to construct a cell model for real-time observation of the dynamic change of glycolysis. It was found that TSA, an HDAC inhibitor, significantly enhanced glycolysis and inhibited cell proliferation. The mechanism of this process was associated with the regulation of HIF-1 alpha and c-Myc.
Article
Materials Science, Biomaterials
Shereen M. Elsherbiny, Changsheng Shao, Adolf Acheampong, Mahmoud A. Khalifa, Chao Liu, Qing Huang
Summary: This study demonstrates the potential of carbon quantum dots derived from broccoli as an effective photodynamic agent in Caenorhabditis elegans models. The carbon quantum dots showed excellent water solubility and optical properties, and exhibited strong photodynamic therapy efficiency by inducing germline apoptosis through the cep-1/p53 pathway.
BIOMATERIALS SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Sung-Yeoul Kim, Vikash Chandra Roy, Jin-Seok Park, Byung-Soo Chun
Summary: This study aims to extract functional substances from Undaria pinnatiida sporophyll using supercritical carbon dioxide (SC-CO2) and subcritical water extraction (SWE). The results showed that the consecutive green extraction method can yield extracts rich in fucoxanthin and beta-carotene, and the sporophyll of Undaria pinnatiida contains health-beneficial fatty acids. Additionally, SWE can extract substances with antioxidant, antibacterial, and antihypertensive activities.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2024)
